Detailed view for TGME49_319920

Basic information

TDR Targets ID: 261662
Toxoplasma gondii, 2-oxo acid dehydrogenases acyltransferase (catalytic domain) domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.762 | Length (AA): 669 | MW (Da): 70290 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00198   2-oxoacid dehydrogenases acyltransferase (catalytic domain)
PF00364   Biotin-requiring enzyme
PF02817   e3 binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016746   transferase activity, transferring acyl groups  
GO:0008415   acyltransferase activity  
GO:0005515   protein binding  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
70 154 1k8m (A) 3 87 48.00 0 1 0.62 -0.04
190 226 1w85 (I) 131 167 51.00 0.0000026 1 0.92 -2.43
256 509 1scz (A) 172 404 29.00 0 1 0.87 -0.79
282 507 1scz (A) 191 402 31.00 0 1 0.88 -1
71 152 1k8m (A) 4 85 50.00 0 1 0.702471 0.12
73 148 1lab (A) 3 78 46.00 0 0.87 0.544802 0.86
173 245 2coo (A) 2 70 39.00 0 1 0.503318 0.08
184 242 1w4i (A) 126 180 51.00 0.00000059 1 0.694091 -0.74
189 225 1w85 (I) 131 167 51.00 0.000065 1 0.822206 -1.65
189 224 2eq7 (C) 131 166 50.00 0 1 0.750012 -1.26
411 665 4n72 (A) 383 628 31.00 0 1 0.735366 -0.27
415 666 1dpb (A) 396 637 30.00 0 1 0.664482 -0.24
428 669 2ii3 (A) 189 421 43.00 0 1 0.887934 -0.65
430 662 3mae (A) 187 409 29.00 0 1 0.725481 -0.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128117)

Species Accession Gene Product
Arabidopsis thaliana AT3G06850   dihydrolipoamide branched chain acyltransferase
Babesia bovis BBOV_II001300   lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial precursor, putative
Brugia malayi Bm1_43910   Lipoamide acyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, mitochondrial precursor
Caenorhabditis elegans CELE_ZK669.4   Protein ZK669.4
Chlamydia trachomatis CT_400   dihydrolipoamide succinyltransferase
Dictyostelium discoideum DDB_G0281797   branched-chain alpha-keto acid dehydrogenase E2 component
Drosophila melanogaster Dmel_CG5599   CG5599 gene product from transcript CG5599-RA
Escherichia coli b0115   pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Homo sapiens ENSG00000137992   dihydrolipoamide branched chain transacylase E2
Leishmania braziliensis LbrM.05.0180   dihydrolipoamide branched chain transacylase, putative
Leishmania donovani LdBPK_050180.1   dihydrolipoamide branched chain transacylase, putative
Leishmania infantum LinJ.05.0180   dihydrolipoamide branched chain transacylase, putative
Leishmania major LmjF.05.0180   dihydrolipoamide branched chain transacylase, putative
Leishmania mexicana LmxM.05.0180   dihydrolipoamide branched chain transacylase, putative
Loa Loa (eye worm) LOAG_15407   hypothetical protein
Loa Loa (eye worm) LOAG_11004   hypothetical protein
Loa Loa (eye worm) LOAG_05887   hypothetical protein
Mus musculus ENSMUSG00000000340   dihydrolipoamide branched chain transacylase E2
Neospora caninum NCLIV_010320   dihydrolipoamide branched chain transacylase, E2 subunit, putative
Oryza sativa 4327289   Os01g0314100
Plasmodium berghei PBANKA_0402300   lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, putative
Plasmodium falciparum PF3D7_0303700   lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
Plasmodium knowlesi PKNH_0839400   lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, putative
Plasmodium vivax PVX_119310   lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, putative
Plasmodium yoelii PY00503   Plasmodium vivax PV1H14105_P
Schmidtea mediterranea mk4.029456.00  
Schmidtea mediterranea mk4.021231.00  
Trypanosoma brucei gambiense Tbg972.5.5850   dihydrolipoamide branched chain transacylase, putative
Trypanosoma brucei Tb927.5.4330   dihydrolipoamide branched chain transacylase, putative
Trypanosoma congolense TcIL3000_5_4970   dihydrolipoamide branched chain transacylase, putative
Trypanosoma cruzi TcCLB.507601.70   dihydrolipoamide branched chain transacylase, putative
Trypanosoma cruzi TcCLB.510351.90   dihydrolipoamide branched chain transacylase, putative
Toxoplasma gondii TGME49_319920   2-oxo acid dehydrogenases acyltransferase (catalytic domain) domain-containing protein
Theileria parva TP04_0457   lipoamide transferase, putative

Essentiality

TGME49_319920 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.4330 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.4330 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.4330 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.4330 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0115 Escherichia coli essential goodall
CELE_ZK669.4 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0402300 Plasmodium berghei Slow plasmo
TGME49_319920 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_319920 (Toxoplasma gondii), 2-oxo acid dehydrogenases acyltransferase (catalytic domain) domain-containing protein
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