Detailed view for TGME49_305940

Basic information

TDR Targets ID: 263102
Toxoplasma gondii, peptidyl-prolyl cis-trans isomerase, cyclophilin-type domain-containing protein
Source Database / ID:  ToxoDB 

pI: 9.303 | Length (AA): 612 | MW (Da): 68859 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00160   Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
PF04641   Rtf2 RING-finger

Gene Ontology

Mouse over links to read term descriptions.
GO:0000413   protein peptidyl-prolyl isomerization  
GO:0000151   ubiquitin ligase complex  
GO:0004842   ubiquitin-protein ligase activity  
GO:0003755   peptidyl-prolyl cis-trans isomerase activity  
GO:0016567   protein ubiquitination  

Metabolic Pathways

Ubiquitin mediated proteolysis (KEGG)

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
42 91 2bay (A) 3 53 22.00 0.00000031 0.3 0.47 -1.72
307 590 1ihg (A) 6 292 25.00 0 0.99 0.58 0.58
315 502 1zkc (A) 276 452 48.00 0 1 0.73 -0.78
321 483 2fu0 (A) 8 158 37.00 0 1 0.74 -0.9
42 90 4wz3 (B) 37 85 35.00 0.08 0.65 0.597865 -1.09
42 82 2ecj (A) 18 58 20.00 0.042 0.06 0.219193 0.24
313 523 5ex1 (A) 30 264 21.00 0 1 0.551171 -0.22
317 488 3bo7 (A) 5 176 99.99 0 1 1.49185 -1.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3BO7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite. Gregory
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129895)

Species Accession Gene Product
Arabidopsis thaliana AT5G67530   peptidyl-prolyl cis-trans isomerase-like 2
Babesia bovis BBOV_II006190   Peptidyl-prolyl cis-trans isomerase 4
Brugia malayi Bm1_21700   cyclophilin-type peptidyl-prolyl cis-trans isomerase-4, Bmcyp-4
Caenorhabditis elegans CELE_F59E10.2   Protein CYN-4
Dictyostelium discoideum DDB_G0284323   Ubox domain-containing protein
Drosophila melanogaster Dmel_CG7747   CG7747 gene product from transcript CG7747-RA
Echinococcus granulosus EgrG_000765500   peptidyl prolyl cis trans isomerase 2
Echinococcus multilocularis EmuJ_000765500   peptidyl prolyl cis trans isomerase 2
Homo sapiens ENSG00000100023   peptidylprolyl isomerase (cyclophilin)-like 2
Loa Loa (eye worm) LOAG_05139   hypothetical protein
Loa Loa (eye worm) LOAG_11100   hypothetical protein
Mus musculus ENSMUSG00000022771   peptidylprolyl isomerase (cyclophilin)-like 2
Neospora caninum NCLIV_070210   peptidyl-prolyl cis-trans isomerase, putative
Oryza sativa 4331972   Os03g0201100
Schistosoma japonicum Sjp_0043890   ko:K10598 peptidyl-prolyl cis-trans isomerase-like 2, putative
Schistosoma mansoni Smp_021160.2   peptidyl-prolyl cis-trans isomerase-like 2 ppil2
Schmidtea mediterranea mk4.001416.05  
Toxoplasma gondii TGME49_305940   peptidyl-prolyl cis-trans isomerase, cyclophilin-type domain-containing protein
Theileria parva TP02_0580   peptidyl-prolyl cis-trans isomerase, putative

Essentiality

TGME49_305940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F59E10.2 Caenorhabditis elegans embryonic lethal wormbase
TGME49_305940 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cyclophilin A 164 aa 33.6% 140 aa Compounds References
Plasmodium falciparum peptidyl-prolyl cis-trans isomerase 171 aa 35.1% 148 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_305940 (Toxoplasma gondii), peptidyl-prolyl cis-trans isomerase, cyclophilin-type domain-containing protein
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