Detailed view for TGME49_273740

Basic information

TDR Targets ID: 263257
Toxoplasma gondii, acetyl-CoA acyltransferase B, putative
Source Database / ID:  ToxoDB 

pI: 8.4855 | Length (AA): 418 | MW (Da): 44402 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00108   Thiolase, N-terminal domain
PF02803   Thiolase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016747   transferase activity, transferring groups other than amino-acyl groups  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 416 1w0i (A) 85 461 12.00 0 1 0.47 0.66
8 417 2c7y (A) 39 440 55.00 0 1 1.63 -1.26
30 416 1m3k (A) 5 392 38.00 0 1 1.46 -1.39
14 415 2iik (B) 22 422 57.00 0 1 1.67062 -0.87
16 415 2c7y (A) 38 438 55.00 0 1 1.63554 -0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128333)

Species Accession Gene Product
Arabidopsis thaliana AT5G48880   3-keto-acyl-CoA thiolase 2
Arabidopsis thaliana AT2G33150   3-ketoacyl-CoA thiolase 2
Arabidopsis thaliana AT1G04710   3-ketoacyl-CoA thiolase 1
Candida albicans CaO19.2046   similar to rat 3-ketoacyl-CoA thiolase A
Candida albicans CaO19.9271   likely thiolase similar to C.tropicalis 3-ketoacyl-CoA thiolase isoenzymes A and B involved in peroxisomal fatty acid beta-oxida
Candida albicans CaO19.9594   similar to rat 3-ketoacyl-CoA thiolase A
Candida albicans CaO19.1704   likely thiolase similar to C.tropicalis 3-ketoacyl-CoA thiolase isoenzymes A and B involved in peroxisomal fatty acid beta-oxida
Candida albicans CaO19.7520   similar to C.tropicalis 3-ketoacyl-CoA thiolase A
Dictyostelium discoideum DDB_G0274339   3-ketoacyl-CoA thiolase
Escherichia coli b3845   3-ketoacyl-CoA thiolase (thiolase I)
Homo sapiens ENSG00000060971   acetyl-CoA acyltransferase 1
Mus musculus ENSMUSG00000010651   acetyl-Coenzyme A acyltransferase 1B
Mus musculus ENSMUSG00000036138   acetyl-Coenzyme A acyltransferase 1A
Mycobacterium tuberculosis Rv1074c   Probable beta-ketoacyl CoA thiolase FadA3
Mycobacterium ulcerans MUL_0207   acetyl-CoA acetyltransferase
Neospora caninum NCLIV_033970   Acetyl-CoA acetyltransferase (EC 2.3.1.9), related
Oryza sativa 4331150   Os02g0817700
Oryza sativa 4348804   Os10g0457600
Saccharomyces cerevisiae YIL160C   acetyl-CoA C-acyltransferase
Toxoplasma gondii TGME49_273740   acetyl-CoA acyltransferase B, putative

Essentiality

TGME49_273740 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1091 Mycobacterium tuberculosis non-essential nmpdr
b3845 Escherichia coli non-essential goodall
TGME49_273740 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_273740 (Toxoplasma gondii), acetyl-CoA acyltransferase B, putative
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