Detailed view for Rv1074c

Basic information

TDR Targets ID: 5977
Mycobacterium tuberculosis, Probable beta-ketoacyl CoA thiolase FadA3

Source Database / ID:  Tuberculist 

pI: 4.667 | Length (AA): 405 | MW (Da): 42655 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00108   Thiolase, N-terminal domain
PF02803   Thiolase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016747   transferase activity, transferring groups other than amino-acyl groups  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 403 4dd5 (A) 6 396 45.00 0 1 1.55436 -0.68
1 404 6arf (A) 4 398 39.00 0 1 1.48483 -0.56
1 404 3goa (A) 1 387 45.00 0 1 1.47683 -0.48
3 403 1wl4 (A) 7 396 38.00 0 1 1.44932 -0.62

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_128333)

Species Accession Gene Product
Arabidopsis thaliana AT5G48880   3-keto-acyl-CoA thiolase 2
Arabidopsis thaliana AT2G33150   3-ketoacyl-CoA thiolase 2
Arabidopsis thaliana AT1G04710   3-ketoacyl-CoA thiolase 1
Candida albicans CaO19.2046   similar to rat 3-ketoacyl-CoA thiolase A
Candida albicans CaO19.9271   likely thiolase similar to C.tropicalis 3-ketoacyl-CoA thiolase isoenzymes A and B involved in peroxisomal fatty acid beta-oxida
Candida albicans CaO19.9594   similar to rat 3-ketoacyl-CoA thiolase A
Candida albicans CaO19.1704   likely thiolase similar to C.tropicalis 3-ketoacyl-CoA thiolase isoenzymes A and B involved in peroxisomal fatty acid beta-oxida
Candida albicans CaO19.7520   similar to C.tropicalis 3-ketoacyl-CoA thiolase A
Dictyostelium discoideum DDB_G0274339   3-ketoacyl-CoA thiolase
Escherichia coli b3845   3-ketoacyl-CoA thiolase (thiolase I)
Homo sapiens ENSG00000060971   acetyl-CoA acyltransferase 1
Mus musculus ENSMUSG00000010651   acetyl-Coenzyme A acyltransferase 1B
Mus musculus ENSMUSG00000036138   acetyl-Coenzyme A acyltransferase 1A
Mycobacterium tuberculosis Rv1074c   Probable beta-ketoacyl CoA thiolase FadA3
Mycobacterium ulcerans MUL_0207   acetyl-CoA acetyltransferase
Neospora caninum NCLIV_033970   Acetyl-CoA acetyltransferase (EC 2.3.1.9), related
Oryza sativa 4331150   Os02g0817700
Oryza sativa 4348804   Os10g0457600
Saccharomyces cerevisiae YIL160C   acetyl-CoA C-acyltransferase
Toxoplasma gondii TGME49_273740   acetyl-CoA acyltransferase B, putative

Essentiality

Rv1074c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1091 this record Mycobacterium tuberculosis non-essential nmpdr
b3845 Escherichia coli non-essential goodall
TGME49_273740 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

228 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1074c (Mycobacterium tuberculosis), Probable beta-ketoacyl CoA thiolase FadA3
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