Detailed view for TGME49_214150

Basic information

TDR Targets ID: 264386
Toxoplasma gondii, mitochondrial inner membrane translocase subunit TIM17, putative
Source Database / ID:  ToxoDB 

pI: 8.907 | Length (AA): 188 | MW (Da): 19551 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02466   Tim17/Tim22/Tim23/Pmp24 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005743   mitochondrial inner membrane  
GO:0008565   protein transporter activity  
GO:0015031   protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 141 1yq5 (A) 197 314 36.00 0.29 0.1 0.73357 1.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128152)

Species Accession Gene Product
Arabidopsis thaliana AT1G72750   translocase inner membrane subunit 23-2
Arabidopsis thaliana AT3G04800   translocase inner membrane subunit 23-3
Arabidopsis thaliana AT1G17530   translocase of inner mitochondrial membrane 23
Babesia bovis BBOV_IV010710   mitochondrial import inner membrane protein, putative
Brugia malayi Bm1_49155   hypothetical protein
Brugia malayi Bm1_48415   hypothetical protein
Brugia malayi Bm1_56190   Mitochondrial import inner membrane translocase subunit Tim17 family protein
Brugia malayi Bm1_47700   Mitochondrial import inner membrane translocase subunit Tim17 family protein
Brugia malayi Bm1_41590   Mitochondrial import inner membrane translocase subunit Tim17 family protein
Candida albicans CaO19.8941   import of proteins into mitochondrial matrix
Candida albicans CaO19.1361   import of proteins into mitochondrial matrix
Caenorhabditis elegans CELE_F15D3.7   Protein F15D3.7
Dictyostelium discoideum DDB_G0286541   hypothetical protein
Drosophila melanogaster Dmel_CG40042   CG40042 gene product from transcript CG40042-RA
Echinococcus multilocularis EmuJ_000445850   mitochondrial import inner membrane translocase subunit TIM23, putative
Homo sapiens 653252   uncharacterized protein LOC653252
Homo sapiens ENSG00000204152   translocase of inner mitochondrial membrane 23 homolog B (yeast)
Homo sapiens ENSG00000265354   translocase of inner mitochondrial membrane 23 homolog (yeast)
Loa Loa (eye worm) LOAG_06567   hypothetical protein
Mus musculus ENSMUSG00000013701   translocase of inner mitochondrial membrane 23
Neospora caninum NCLIV_051400   hypothetical protein
Oryza sativa 4349135   Os10g0519700
Oryza sativa 4331390   Os03g0114900
Plasmodium berghei PBANKA_1132500   mitochondrial import inner membrane translocase subunit TIM23, putative
Plasmodium falciparum PF3D7_1356200   mitochondrial import inner membrane translocase subunit TIM23, putative
Plasmodium knowlesi PKNH_1115300   mitochondrial import inner membrane translocase subunit TIM23, putative
Plasmodium vivax PVX_114790   mitochondrial import inner membrane translocase subunit TIM23, putative
Plasmodium yoelii PY04920   hypothetical protein
Saccharomyces cerevisiae YNR017W   protein transporter TIM23
Schistosoma japonicum Sjp_0204380   Mitochondrial import inner membrane translocase subunit Tim23, putative
Schistosoma mansoni Smp_080980.2   hypothetical protein
Schmidtea mediterranea mk4.000890.04   Mitochondrial import inner membrane translocase subunit Tim23
Toxoplasma gondii TGME49_214150   mitochondrial inner membrane translocase subunit TIM17, putative
Theileria parva TP01_0733   mitochondrial import inner membrane translocase, putative

Essentiality

TGME49_214150 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F15D3.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_F15D3.7 Caenorhabditis elegans slow growth wormbase
CELE_F15D3.7 Caenorhabditis elegans sterile wormbase
YNR017W Saccharomyces cerevisiae inviable yeastgenome
TGME49_214150 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Saccharomyces cerevisiae protein transporter TIM23 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0049 1 0.5
0.0043 0.4798 1
0.0049 0.4301 0.5
0.0049 1 1
0.0047 0.4112 0.4112
0.0049 1 0.5
0.0049 0.3528 0
0.0083 0.2678 0
0.0049 0.3528 0
0.013 0.6126 1
0.0131 0.3606 1
0.0049 1 0.5
0.0049 1 0.5
0.0127 0.842 1
0.0049 0.7671 0.8097
0.0049 0.3086 0
0.0073 0.2544 0
0.0043 0.3528 0
0.0049 0.368 0.843
0.0131 0.5472 1
0.0049 0.3137 0
0.0049 1 0.5
0.004 0.516 0
0.0049 0.3137 0
0.0049 1 0.5
0.0127 0.842 1
0.0049 0.2784 0.7905
0.0167 0.2609 0.8931
0.0042 0.2938 0.2636
0.0049 0.5205 1
0.0049 0.3695 0.8094
0.0073 0.2544 0
0.0049 0.3528 0
0.0167 0.2609 0.8931
0.0049 0.3241 0.6862
0.0167 0.2609 0.8931
0.004 0.2516 0.8354
0.0031 0.3528 0
0.0042 1 0.5
0.0042 0.2923 0.3143
0.0049 0.3695 0.8094

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_214150 (Toxoplasma gondii), mitochondrial inner membrane translocase subunit TIM17, putative
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