Detailed view for LmjF.35.3210

Basic information

TDR Targets ID: 27472
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8617 | Length (AA): 664 | MW (Da): 73904 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:0005509   calcium ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
452 588 2f3y (A) 3 148 20.00 0 0.98 0.45 -0.39
531 587 1qx2 (A) 3 72 16.00 0.000027 0.48 0.22 -1.72
274 629 4mew (A) 138 481 31.00 0 1 0.925545 -0.62
364 626 4i5j (A) 183 441 38.00 0 1 0.889484 -0.99
426 559 1gjy (A) 67 193 16.00 0 0.14 0.172207 0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127605)

Species Accession Gene Product
Arabidopsis thaliana AT5G28900   probable serine/threonine protein phosphatase 2A regulatory subunit B''gamma
Arabidopsis thaliana AT1G03960   calcium-binding EF-hand-containing protein
Arabidopsis thaliana AT5G28850   serine/threonine protein phosphatase 2A regulatory subunit B''beta
Arabidopsis thaliana AT5G44090   serine/threonine protein phosphatase 2A regulatory subunit B''alpha
Arabidopsis thaliana AT1G54450   calcium-binding EF-hand-containing protein
Brugia malayi Bm1_36600   EF hand family protein
Brugia malayi Bm1_11800   protein phosphatase 2A
Caenorhabditis elegans CELE_C06G1.5   Protein C06G1.5, isoform A
Cryptosporidium hominis Chro.70554   CG4733-PA
Cryptosporidium parvum cgd7_4970   protein phosphatase 2A regulatory subunit (contains a conserved version of EF hands)
Dictyostelium discoideum DDB_G0279813   hypothetical protein
Drosophila melanogaster Dmel_CG4733   CG4733 gene product from transcript CG4733-RD
Echinococcus granulosus EgrG_000875600   serine:threonine protein phosphatase 2A
Echinococcus multilocularis EmuJ_000875600   serine:threonine protein phosphatase 2A
Giardia lamblia GL50803_17538   Phosphoprotein phosphatase 2A regulatory subunit
Giardia lamblia GL50803_9894   Protein phosphatase 2A regulatory subunit, putative
Homo sapiens ENSG00000073711   protein phosphatase 2, regulatory subunit B'', alpha
Homo sapiens 102725016   serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta-like
Homo sapiens 102724991   serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta-like
Homo sapiens ENSG00000167393   protein phosphatase 2, regulatory subunit B'', beta
Leishmania braziliensis LbrM.34.3120   hypothetical protein, conserved
Leishmania braziliensis LbrM.17.0650   hypothetical protein, conserved
Leishmania donovani LdBPK_170730.1   EF-hand domain pair, putative
Leishmania donovani LdBPK_353260.1   EF-hand domain pair, putative
Leishmania infantum LinJ.35.3260   hypothetical protein, conserved
Leishmania infantum LinJ.17.0730   hypothetical protein, conserved
Leishmania major LmjF.35.3210   hypothetical protein, conserved
Leishmania major LmjF.17.0750   hypothetical protein, conserved
Leishmania mexicana LmxM.17.0750   hypothetical protein, conserved
Leishmania mexicana LmxM.34.3210   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05562   protein phosphatase 2A
Loa Loa (eye worm) LOAG_10207   hypothetical protein
Loa Loa (eye worm) LOAG_10206   hypothetical protein
Loa Loa (eye worm) LOAG_08349   hypothetical protein
Mus musculus ENSMUSG00000043154   protein phosphatase 2, regulatory subunit B'', alpha
Mus musculus ENSMUSG00000093803   protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta
Neospora caninum NCLIV_038240   hypothetical protein
Oryza sativa 4328580   Os02g0191700
Oryza sativa 4348893   Os10g0476600
Plasmodium berghei PBANKA_1132700   phosphatase 2A regulatory subunit-related protein, putative
Plasmodium falciparum PF3D7_1356400   phosphatase 2A regulatory subunit-related protein, putative
Plasmodium knowlesi PKNH_1115100   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_114800   hypothetical protein, conserved
Plasmodium yoelii PY01608   Arabidopsis thaliana At5g28850/F7P1_30
Schistosoma japonicum Sjp_0312740   Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta, putative
Schistosoma japonicum Sjp_0133710   Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha, putative
Schistosoma japonicum Sjp_0089540   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma mansoni Smp_089930   phosphatase 2a regulatory subunit-related
Schmidtea mediterranea mk4.000908.01   Phosphatase 2a regulatory subunit-related
Schmidtea mediterranea mk4.012041.00  
Schmidtea mediterranea mk4.012041.04  
Schmidtea mediterranea mk4.017609.00  
Trypanosoma brucei gambiense Tbg972.7.7950   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.9.7530   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0895   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.6810   EF-hand domain pair, putative
Trypanosoma brucei Tb927.9.12340   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_9_5120   EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.510741.30   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510661.240   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510057.4   EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.503967.4   EF-hand domain pair, putative
Trypanosoma cruzi TcCLB.508909.170   EF-hand domain pair, putative
Toxoplasma gondii TGME49_200400   hypothetical protein
Trichomonas vaginalis TVAG_070200   serine/threonine-protein phosphatase 2A 48 kDa regulatory subunit B, putative

Essentiality

LmjF.35.3210 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.3350 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.3350 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.3350 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3350 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.6810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.6810 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.6810 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.6810 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1132700 Plasmodium berghei Essential plasmo
TGME49_200400 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.35.3210 (Leishmania major), hypothetical protein, conserved
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