Detailed view for LmjF.36.0600

Basic information

TDR Targets ID: 28809
Leishmania major, ubiquitin/ribosomal protein S27a, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1557 | Length (AA): 152 | MW (Da): 16997 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00240   Ubiquitin family
PF01599   Ribosomal protein S27a

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0005515   protein binding  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 75 1v2y (A) 8 104 25.00 0 1 0.6 -0.87
1 67 1z2m (A) 82 154 34.00 0 1 0.93 -1.78
1 69 1c3t (A) 1 75 42.00 0 1 1.01 -1.28
1 70 2jvc (A) 6 81 40.00 0.000000026 1 0.841426 0.33
1 69 5jg6 (B) 1 75 35.00 0.00034 1 0.870847 -0.53
1 84 3q3f (A) 105 188 36.00 0 1 0.825732 0.24
77 151 4kzy (f) 82 152 54.00 0.000000000072 0.88 0.955521 1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127221)

Species Accession Gene Product
Arabidopsis thaliana AT1G23410   40S ribosomal protein S27a-1
Arabidopsis thaliana AT2G47110   ubiquitin-40S ribosomal protein S27a-2
Arabidopsis thaliana AT3G62250   ubiquitin-40S ribosomal protein S27a-3
Babesia bovis BBOV_III008500   ribosomal protein S27a family protein
Brugia malayi Bm1_09050   ubiquitin
Candida albicans CaO19.10599   ubiquitin-ribosomal protein fusion that gives rise to cytoplasmic ribosomal small subunit protein S27a
Candida albicans CaO19.3087   ubiquitin-ribosomal protein fusion that gives rise to cytoplasmic ribosomal small subunit protein S27a
Caenorhabditis elegans CELE_H06I04.4   Protein UBL-1, isoform A
Cryptosporidium hominis Chro.40350   ubiquitin
Cryptosporidium parvum cgd4_3080   ribosomal protein S27a, ubiquitin plus zincribbon, UB13p
Dictyostelium discoideum DDB_G0276765   ubiquitin/ribosomal protein S27a fusion protein
Drosophila melanogaster Dmel_CG5271   Ribosomal protein S27A
Echinococcus granulosus EgrG_000785600   ubiquitin 40S ribosomal protein S27a
Entamoeba histolytica EHI_036530   40S ribosomal protein S27a, putative
Echinococcus multilocularis EmuJ_000785600   ubiquitin 40S ribosomal protein S27a
Giardia lamblia GL50803_16298   Hypothetical protein
Homo sapiens ENSG00000143947   ribosomal protein S27a
Leishmania braziliensis LbrM.35.0720   ubiquitin/ribosomal protein S27a, putative
Leishmania braziliensis LbrM.14.1440   ubiquitin/ribosomal protein S27a, putative
Leishmania donovani LdBPK_360660.1   ubiquitin/ribosomal protein S27a, putative
Leishmania infantum LinJ.36.0660   ubiquitin/ribosomal protein S27a, putative
Leishmania infantum LinJ.14.1350   ubiquitin/ribosomal protein S27a, putative
Leishmania major LmjF.14.1270   ubiquitin/ribosomal protein S27a, putative
Leishmania major LmjF.36.0600   ubiquitin/ribosomal protein S27a, putative
Leishmania mexicana LmxM.36.0600   ubiquitin/ribosomal protein S27a, putative
Leishmania mexicana LmxM.14.1270   ubiquitin/ribosomal protein S27a, putative
Loa Loa (eye worm) LOAG_04017   ubiquitin
Mus musculus ENSMUSG00000020460   ribosomal protein S27A
Neospora caninum NCLIV_062890   hypothetical protein
Oryza sativa 4326977   Os01g0328400
Oryza sativa 4337883   Os05g0160200
Plasmodium berghei PBANKA_1039400   ubiquitin-40S ribosomal protein S27a, putative
Plasmodium falciparum PF3D7_1402500   ubiquitin-40S ribosomal protein S27a, putative
Plasmodium knowlesi PKNH_1355500   ubiquitin-40S ribosomal protein S27a, putative
Plasmodium vivax PVX_086305   ubiquitin-40S ribosomal protein S27a, putative
Plasmodium yoelii PY03337   ubiquitin
Plasmodium yoelii PY00122   ubiquitin c-terminal extension protein UBIcep86
Saccharomyces cerevisiae YLR167W   ubiquitin-ribosomal 40S subunit protein S31 fusion protein
Schistosoma japonicum Sjp_0209330   40S ribosomal protein S27a, putative
Schistosoma mansoni Smp_017280   ubiquitin
Schmidtea mediterranea mk4.000756.01   Putative ubiquitin
Schmidtea mediterranea mk4.005119.00   Ubiquitin-40S ribosomal protein S27a
Trypanosoma brucei gambiense Tbg972.7.4100   ubiquitin/ribosomal protein S27a, putative
Trypanosoma brucei gambiense Tbg972.10.6090   ubiquitin/ribosomal protein S27a, putative
Trypanosoma brucei Tb927.7.3680   ubiquitin/ribosomal protein S27a, putative
Trypanosoma brucei Tb927.10.5030   ubiquitin/ribosomal protein S27a, putative
Trypanosoma congolense TcIL3000_7_2940   ubiquitin/ribosomal protein S27a, putative
Trypanosoma congolense TcIL3000_10_4210   ubiquitin/ribosomal protein S27a, putative
Trypanosoma cruzi TcCLB.510409.39   ubiquitin/ribosomal protein S27a, putative
Trypanosoma cruzi TcCLB.509937.100   ubiquitin/ribosomal protein S27a, putative
Trypanosoma cruzi TcCLB.508661.20   ubiquitin/ribosomal protein S27a, putative
Trypanosoma cruzi TcCLB.510293.40   ubiquitin/ribosomal protein S27a, putative
Toxoplasma gondii TGME49_245620   ribosomal-ubiquitin protein RPS27A
Theileria parva TP04_0752   hypothetical protein
Trichomonas vaginalis TVAG_133150   ubiquitin, putative
Trichomonas vaginalis TVAG_055640   ubiquitin, putative
Trichomonas vaginalis TVAG_452780   ubiquitin, putative
Trichomonas vaginalis TVAG_478140   ubiquitin, putative
Trichomonas vaginalis TVAG_031870   ubiquitin, putative

Essentiality

LmjF.36.0600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3680 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.3680 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.3680 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.3680 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.5030 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.5030 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.5030 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5030 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_H06I04.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_H06I04.4 Caenorhabditis elegans larval arrest wormbase
CELE_H06I04.4 Caenorhabditis elegans slow growth wormbase
CELE_H06I04.4 Caenorhabditis elegans sterile wormbase
YLR167W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1039400 Plasmodium berghei Essential plasmo
TGME49_245620 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.36.0600 (Leishmania major), ubiquitin/ribosomal protein S27a, putative
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