Detailed view for TcCLB.506407.10

Basic information

TDR Targets ID: 31824
Trypanosoma cruzi, lysosomal alpha-mannosidase precursor, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8837 | Length (AA): 977 | MW (Da): 111196 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01074   Glycosyl hydrolases family 38 N-terminal domain
PF07748   Glycosyl hydrolases family 38 C-terminal domain
PF09261   Alpha mannosidase middle domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008270   zinc ion binding  
GO:0004559   alpha-mannosidase activity  
GO:0003824   catalytic activity  
GO:0030246   carbohydrate binding  
GO:0015923   mannosidase activity  
GO:0006013   mannose metabolic process  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

Other glycan degradation (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 971 1qwn (A) 65 1040 24.00 0 1 1.23 -0.63
27 310 1o7d (A) 65 341 48.00 0 1 0.92 -1.07
17 971 3bvx (A) 73 1040 23.00 0 1 1.18138 0
22 311 1o7d (A) 60 342 46.00 0 1 0.830727 -0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_129192)

Species Accession Gene Product
Arabidopsis thaliana AT5G13980   Glycosyl hydrolase family 38 protein
Arabidopsis thaliana AT3G26720   glycosyl hydrolase family 38 protein
Arabidopsis thaliana AT5G66150   Glycosyl hydrolase family protein
Caenorhabditis elegans CELE_F55D10.1   Protein AMAN-1
Dictyostelium discoideum DDB_G0292206   hypothetical protein
Drosophila melanogaster Dmel_CG5322   CG5322 gene product from transcript CG5322-RB
Drosophila melanogaster Dmel_CG9466   CG9466 gene product from transcript CG9466-RB
Drosophila melanogaster Dmel_CG9465   CG9465 gene product from transcript CG9465-RB
Drosophila melanogaster Dmel_CG6206   Lysosomal alpha-mannosidase
Drosophila melanogaster Dmel_CG9463   CG9463 gene product from transcript CG9463-RB
Drosophila melanogaster Dmel_CG9468   CG9468 gene product from transcript CG9468-RA
Echinococcus granulosus EgrG_000704400   lysosomal alpha mannosidase
Echinococcus multilocularis EmuJ_000704400   lysosomal alpha mannosidase
Homo sapiens ENSG00000104774   mannosidase, alpha, class 2B, member 1
Loa Loa (eye worm) LOAG_00101   hypothetical protein
Mus musculus ENSMUSG00000005142   mannosidase 2, alpha B1
Oryza sativa 4350617   Os11g0525600
Oryza sativa 4348080   Os10g0140200
Schistosoma japonicum Sjp_0032110   Lysosomal alpha-mannosidase precursor, putative
Schistosoma japonicum Sjp_0032090   ko:K01191 alpha-mannosidase [EC3.2.1.24], putative
Schistosoma mansoni Smp_131580   lysosomal alpha-mannosidase (mannosidase alpha class 2b member 1)
Schmidtea mediterranea mk4.002033.03  
Schmidtea mediterranea mk4.020205.00  
Trypanosoma brucei gambiense Tbg972.10.3200   lysosomal alpha-mannosidase precursor, putative
Trypanosoma brucei Tb11.v5.0498   lysosomal alpha-mannosidase precursor, putative
Trypanosoma brucei Tb927.10.2570   lysosomal alpha-mannosidase precursor, putative
Trypanosoma cruzi TcCLB.506195.120   lysosomal alpha-mannosidase precursor, putative
Trypanosoma cruzi TcCLB.506407.10   lysosomal alpha-mannosidase precursor, putative

Essentiality

TcCLB.506407.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2570 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.2570 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.2570 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.2570 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens mannosidase, alpha, class 2B, member 1 Compounds References
Canavalia ensiformis Alpha-mannosidase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0054 0.3793 0.5
0.0098 0.4701 0.5
0.0086 0.5426 1
0.0098 0.4701 0.5
0.0382 0.4473 0.5
0.0054 0.3793 0.5
0.0054 0.3793 0.5
0.0123 0.4169 1
0.0144 0.4234 0.5
0.0084 0.5231 1
0.0054 0.3793 0.5
0.0098 0.4701 0.5

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma cruzi ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    TcCLB.506407.10 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma cruzi ( 1 )

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.506407.10 (Trypanosoma cruzi), lysosomal alpha-mannosidase precursor, putative
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