pI: 5.1347 |
Length (AA): 602 |
MW (Da): 67711 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Modbase 3D models:
There are 9 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
---|---|---|---|---|---|---|---|---|---|
16 | 276 | 2ivx (A) | 7 | 261 | 11.00 | 0 | 0.97 | 0.32 | -0.94 |
28 | 264 | 1g3n (C) | 16 | 252 | 10.00 | 0 | 0.93 | 0.44 | -1 |
40 | 293 | 1jkw () | 13 | 281 | 14.00 | 0 | 1 | 0.46 | -0.44 |
74 | 264 | 1ais (B) | 1108 | 1298 | 23.00 | 0 | 1 | 0.72 | -1.92 |
78 | 378 | 4roc (A) | 72 | 406 | 17.00 | 0 | 1 | 0.5859 | 0.24 |
82 | 254 | 1ais (B) | 1116 | 1288 | 22.00 | 0 | 1 | 0.653275 | -0.78 |
85 | 254 | 1ais (B) | 1119 | 1288 | 28.00 | 0.0000029 | 1 | 0.655792 | -0.59 |
368 | 596 | 4tql (A) | 10 | 235 | 11.00 | 0.0000067 | 0.03 | 0.465299 | -0.47 |
387 | 571 | 4uos (A) | 1 | 185 | 7.00 | 0.012 | 0.01 | 0.452209 | -1.09 |
Help me make sense of these data.
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Upregulation Percent | Ranking | Stage | Dataset |
---|---|---|---|
Upper 80-100% percentile | metacyclic. | Smircich P |
Upregulation Percent | Ranking | Stage | Dataset |
---|---|---|---|
Upper 60-80% percentile | epimastigote. | Smircich P |
Smircich P | Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi. |
Ortholog group members (OG5_127701)
Species | Accession | Gene Product |
---|---|---|
Arabidopsis thaliana | AT2G45100 | Cyclin/Brf1-like TBP-binding protein |
Arabidopsis thaliana | AT2G01280 | Cyclin/Brf1-like TBP-binding protein |
Arabidopsis thaliana | AT3G09360 | Cyclin/Brf1-like TBP-binding protein |
Babesia bovis | BBOV_IV002720 | transcription factor TFIIB subunit, putative |
Brugia malayi | Bm1_25435 | Transcription factor TFIIB repeat family protein |
Candida albicans | CaO19.13970 | similar to S. cerevisiae BRF1 (YGR246C) component of RNA Pol III transcription factor TFIIIB complex |
Candida albicans | CaO19.6649 | similar to S. cerevisiae BRF1 (YGR246C) component of RNA Pol III transcription factor TFIIIB complex |
Caenorhabditis elegans | CELE_F45E12.2 | Protein BRF-1 |
Cryptosporidium hominis | Chro.50018 | transcription factor IIIb subunit |
Cryptosporidium parvum | cgd5_3540 | cyclin domain protein |
Dictyostelium discoideum | DDB_G0281215 | TATA box-binding protein-associated factor, RNA polymerase III, subunit 2 |
Drosophila melanogaster | Dmel_CG31256 | CG31256 gene product from transcript CG31256-RA |
Echinococcus granulosus | EgrG_000229600 | transcription factor iiib 90 kda subunit |
Entamoeba histolytica | EHI_158020 | transcription initiation factor IIIB chain BRF, putative |
Echinococcus multilocularis | EmuJ_000229600 | transcription factor iiib 90 kda subunit |
Giardia lamblia | GL50803_4125 | Transcription factor IIIB 70 kDa subunit BRF |
Homo sapiens | ENSG00000185024 | BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit |
Leishmania braziliensis | LbrM.25.0390 | transcription factor, putative |
Leishmania donovani | LdBPK_250450.1 | transcription factor IIIb, putative |
Leishmania infantum | LinJ.25.0450 | transcription factor, putative |
Leishmania major | LmjF.25.0440 | transcription factor, putative |
Leishmania mexicana | LmxM.25.0440 | transcription factor, putative |
Loa Loa (eye worm) | LOAG_07165 | BRF1 protein |
Mus musculus | ENSMUSG00000011158 | BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) |
Neospora caninum | NCLIV_002820 | hypothetical protein |
Oryza sativa | 4338362 | Os05g0305100 |
Plasmodium berghei | PBANKA_1313000 | transcription factor IIIb subunit, putative |
Plasmodium falciparum | PF3D7_1449300 | transcription factor IIIb subunit, putative |
Plasmodium knowlesi | PKNH_1232800 | transcription factor IIIb subunit, putative |
Plasmodium vivax | PVX_118025 | transcription factor IIIb subunit, putative |
Plasmodium yoelii | PY07313 | transcription factor iiib 70 kDa subunit |
Saccharomyces cerevisiae | YGR246C | Brf1p |
Schistosoma japonicum | Sjp_0094040 | similar to Transcription factor IIIB 90 kDa subunit, putative |
Schistosoma japonicum | Sjp_0094050 | expressed protein |
Schistosoma mansoni | Smp_071570 | transcription initiation factor brf1 |
Schmidtea mediterranea | mk4.000180.12 | Transcription factor IIIB 90 kDa subunit |
Schmidtea mediterranea | mk4.005392.00 | Transcription factor IIIB 90 kDa subunit |
Trypanosoma brucei gambiense | Tbg972.11.380 | transcription factor, putative,transcription factor IIIb, putative |
Trypanosoma brucei | Tb927.11.470 | TFIIB-related factor BRF1 |
Trypanosoma congolense | TcIL3000.11.410 | transcription factor IIIb, putative |
Trypanosoma cruzi | TcCLB.507093.180 | transcription factor IIIb, putative |
Toxoplasma gondii | TGME49_207900 | transcription initiation factor TFIIIB |
Theileria parva | TP03_0376 | transcription factor IIIb subunit, putative |
Trichomonas vaginalis | TVAG_139840 | transcription initiation factor brf1, putative |
Gene/Ortholog | Organism | Phenotype | Source Study |
---|---|---|---|
Tb11.03.0670 | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (3 days) | alsford |
Tb11.03.0670 | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (6 days) | alsford |
Tb11.03.0670 | Trypanosoma brucei | no significant loss or gain of fitness in procyclic forms | alsford |
Tb11.03.0670 | Trypanosoma brucei | significant loss of fitness in differentiation of procyclic to bloodstream forms | alsford |
CELE_F45E12.2 | Caenorhabditis elegans | larval arrest | wormbase |
YGR246C | Saccharomyces cerevisiae | inviable | yeastgenome |
TGME49_207900 | Toxoplasma gondii | Probably essential | sidik |
alsford | High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome | Genome Res 2011, 21:915-924 |
blattner | Systematic mutagenesis of the E. coli (MG1655) genome | J Bacteriol 2004, 186:4921-4930 |
yeastgenome | Systematic deletion of yeast genes | Saccharomyces Genome Database |
nmpdr | Genome-scale essentiality datasets from published studies (M. tuberculosis) | National Microbial Pathogen Data Resource |
neb | C. elegans RNAi phenotypes | Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs |
gerdes | Experimental determination and system-level analysis of essential genes in E. coli MG1655 | Gerdes et al., J Bacteriol. 2003 185:5673-84 |
shigen | Profiling of E. coli Chromosome (PEC) | National Institute of Genetics, Japan |
wormbase | C. elegans RNAi experiments | WormBase web site, http://www.wormbase.org, release WS170 |
keio | Systematic single-gene knock-out mutants of E. coli K12 | The Keio Collection |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.