Detailed view for PF3D7_0510200

Basic information

TDR Targets ID: 4133
Plasmodium falciparum, peptidyl-prolyl cis-trans isomerase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.2934 | Length (AA): 747 | MW (Da): 87040 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00160   Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Gene Ontology

Mouse over links to read term descriptions.
GO:0000413   protein peptidyl-prolyl isomerization  
GO:0016018   cyclosporin A binding  
GO:0005515   protein binding  
GO:0003755   peptidyl-prolyl cis-trans isomerase activity  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 398 1nex (B) 309 738 13.00 0 0.73 0.55 0.68
142 441 2h14 (A) 33 330 17.00 0 1 0.67 -0.67
592 746 2fu0 (A) 5 159 99.99 0 1 1.48 -1.88
5 138 1c1g (A) 23 154 14.00 0.41 0 0.335584 -0.47
592 746 2fu0 (A) 5 159 99.99 0 1 1.5027 -2
600 725 3k2c (A) 19 147 48.00 0 1 0.793875 -0.9
602 726 4hy7 (A) 18 152 55.00 0 1 0.830536 -1.08
593 747 2fu0 (A) 5 159 99.99 0 1 1.50212 -2
601 726 3k2c (A) 19 147 48.00 0 1 0.793349 -0.9
603 727 4hy7 (A) 18 152 55.00 0 1 0.830012 -1.08

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2FU0:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile early ring. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, merozoite, sporozoite, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_128211)

Species Accession Gene Product
Arabidopsis thaliana AT3G44600   cyclophilin71
Babesia bovis BBOV_IV009550   peptidyl-prolyl cis-trans isomerase, putative
Brugia malayi Bm1_28740   cyclophilin-type peptidyl-prolyl cis-trans isomerase-15, Bmcyp-15
Caenorhabditis elegans CELE_Y87G2A.6   Protein CYN-15
Cryptosporidium hominis Chro.70067   protein-related
Cryptosporidium parvum cgd7_520   cyclincyclophilin like peptidyl-prolyl cis-trans isomerase fused to WD40 repeats at the N-terminus
Dictyostelium discoideum DDB_G0269054   WD40 repeat-containing protein
Drosophila melanogaster Dmel_CG3511   CG3511 gene product from transcript CG3511-RA
Escherichia coli b0525   peptidyl-prolyl cis-trans isomerase B (rotamase B)
Echinococcus granulosus EgrG_000596800   peptidylprolyl isomerase domain and WD40 repeat
Echinococcus multilocularis EmuJ_000596800   peptidylprolyl isomerase domain and WD40 repeat
Homo sapiens ENSG00000113593   peptidylprolyl isomerase domain and WD repeat containing 1
Loa Loa (eye worm) LOAG_13806   cyclophilin-type peptidyl-prolyl cis-trans isomerase-15
Loa Loa (eye worm) LOAG_09305   hypothetical protein
Mycobacterium leprae ML0011   Probable peptidyl-prolyl cis-trans isomerase PpiA (PPIase A) (Rotamase A)
Mus musculus ENSMUSG00000021713   peptidylprolyl isomerase domain and WD repeat containing 1
Mycobacterium tuberculosis Rv0009   Probable iron-regulated peptidyl-prolyl cis-trans isomerase A PpiA (PPIase A) (rotamase A)
Mycobacterium ulcerans MUL_0013   peptidyl-prolyl cis-trans isomerase A, PpiA
Neospora caninum NCLIV_009630   cyclophilin, putative
Oryza sativa 4346298   Os08g0557500
Plasmodium berghei PBANKA_1109800   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium falciparum PF3D7_0510200   peptidyl-prolyl cis-trans isomerase
Plasmodium knowlesi PKNH_1023400   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium vivax PVX_098070   cyclophilin, putative
Plasmodium yoelii PY03899   putative protein-related
Schistosoma japonicum Sjp_0088530   Peptidylprolyl isomerase domain and WD repeat-containing protein 1, putative
Schistosoma japonicum Sjp_0200300   ko:K01802 peptidylprolyl isomerase [EC5.2.1.8], putative
Schistosoma mansoni Smp_133820   peptidyl-prolyl cis-trans isomerase-like
Schmidtea mediterranea mk4.007066.04  
Schmidtea mediterranea mk4.007066.03  
Schmidtea mediterranea mk4.042814.00  
Schmidtea mediterranea mk4.007376.01  
Schmidtea mediterranea mk4.007376.00  
Toxoplasma gondii TGME49_320640   peptidylprolyl isomerase domain-containing protein
Treponema pallidum TP0947   peptidyl-prolyl cis-trans isomerase, cyclophilin-binding (cyp)
Theileria parva TP01_0815   cyclophilin, putative
Trichomonas vaginalis TVAG_172150   peptidyl-prolyl cis-trans isomerase, putative

Essentiality

PF3D7_0510200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu9 Mycobacterium tuberculosis non-essential nmpdr
CELE_Y87G2A.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y87G2A.6 Caenorhabditis elegans sterile wormbase
PBANKA_1109800 Plasmodium berghei Slow plasmo
TGME49_320640 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum peptidyl-prolyl cis-trans isomerase 171 aa 48.9% 137 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for FK-Binding Protein Peptidyl-Prolyl Isomerase Activity (5.2.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_0510200 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Plasmodium falciparum ( 3 )

Bibliographic References

13 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0510200 (Plasmodium falciparum), peptidyl-prolyl cis-trans isomerase
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