Detailed view for PF3D7_0528700

Basic information

TDR Targets ID: 4317
Plasmodium falciparum, peptidyl-prolyl cis-trans isomerase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.3064 | Length (AA): 204 | MW (Da): 23199 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00160   Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Gene Ontology

Mouse over links to read term descriptions.
GO:0016018   cyclosporin A binding  
GO:0000413   protein peptidyl-prolyl isomerization  
GO:0003755   peptidyl-prolyl cis-trans isomerase activity  

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 199 2b71 (A) 24 191 90.00 0 1 2.03 -2.14
31 199 2b71 (A) 23 191 89.00 0 1 2.00423 -1.94

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2B71:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, Oocyst, Ring, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 48 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile gametocyte, late trophozoite. PlasmoDB
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127991)

Species Accession Gene Product
Arabidopsis thaliana AT2G36130   peptidyl-prolyl cis-trans isomerase-like 1
Brugia malayi Bm1_08840   cyclophilin-type peptidyl-prolyl cis-trans isomerase-12, Bmcyp-12
Candida albicans CaO19.1552   likely Cyclophilin type peptidyl-prolyl cis-trans isomerase similar to C. albicans CYP1 (CaP19.6472) and to S. cerevisiae CPR3 (
Candida albicans CaO19.9126   likely Cyclophilin type peptidyl-prolyl cis-trans isomerase similar to C. albicans CYP1 (CaP19.6472) and to S. cerevisiae CPR3 (
Caenorhabditis elegans CELE_C34D4.12   Protein CYN-12
Dictyostelium discoideum DDB_G0269216   cyclophilin-type peptidylprolyl cis-trans isomerase
Drosophila melanogaster Dmel_CG13892   Cyclophilin-like
Escherichia coli b3363   peptidyl-prolyl cis-trans isomerase A (rotamase A)
Echinococcus granulosus EgrG_000992200   peptidyl prolyl cis trans isomerase 1
Echinococcus multilocularis EmuJ_000992200   peptidyl prolyl cis trans isomerase 1
Giardia lamblia GL50803_17000   Peptidyl-prolyl cis-trans isomerase B precursor
Homo sapiens ENSG00000137168   peptidylprolyl isomerase (cyclophilin)-like 1
Loa Loa (eye worm) LOAG_10735   hypothetical protein
Mus musculus ENSMUSG00000024007   peptidylprolyl isomerase (cyclophilin)-like 1
Neospora caninum NCLIV_036200   cyclophilin, putative
Oryza sativa 4346316   Os08g0559400
Plasmodium berghei PBANKA_1243300   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium falciparum PF3D7_0528700   peptidyl-prolyl cis-trans isomerase
Plasmodium knowlesi PKNH_1004000   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium vivax PVX_079815   cyclophilin, putative
Plasmodium yoelii PY00693   cyclophilin-like protein
Saccharomyces cerevisiae YML078W   peptidylprolyl isomerase CPR3
Schistosoma japonicum Sjp_0017950   ko:K01802 peptidylprolyl isomerase [EC5.2.1.8], putative
Schistosoma mansoni Smp_029900.1   peptidyl-prolyl cis-trans isomerase-like 1 ppil1
Schistosoma mansoni Smp_029900.2   peptidyl-prolyl cis-trans isomerase-like 1 ppil1
Schmidtea mediterranea mk4.007070.00   Peptidyl-prolyl cis-trans isomerase-like 1
Toxoplasma gondii TGME49_270560   peptidyl-prolyl cis-trans isomerase family 1

Essentiality

PF3D7_0528700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3363 Escherichia coli non-essential goodall
CELE_C34D4.12 Caenorhabditis elegans embryonic lethal wormbase
CELE_C34D4.12 Caenorhabditis elegans larval arrest wormbase
CELE_C34D4.12 Caenorhabditis elegans sterile wormbase
TGME49_270560 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for FK-Binding Protein Peptidyl-Prolyl Isomerase Activity (5.2.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Plasmodium falciparum ( 1 )

Reagent availability

  • Pfal008572AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Pfal008572; Recombinant protein: full-length; Source: P falciparum; cyclophilin, putative ;
  • Reagent:
  • Target Type Source Notes
    PF3D7_0528700 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Plasmodium falciparum ( 2 )

Bibliographic References

13 literature references were collected for this gene.

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Gene identifier PF3D7_0528700 (Plasmodium falciparum), peptidyl-prolyl cis-trans isomerase
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