Detailed view for TcCLB.511127.360

Basic information

TDR Targets ID: 46979
Trypanosoma cruzi, tetratricopeptide repeat protein 8
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3153 | Length (AA): 661 | MW (Da): 74145 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13181   Tetratricopeptide repeat
PF14559   Tetratricopeptide repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0034464   GO:BBSome  

GO:1905515   GO:non-motile cilium assembly  

GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
303 661 1w3b (A) 14 367 17.00 0 1 0.86 -0.69
580 659 1na3 (A) 7 86 24.00 0.000000000086 1 0.7 -2.56
97 302 5cwi (A) 14 227 10.00 0.29 0.06 0.411649 -0.59
139 411 3cvp (A) 338 622 16.00 0.00044 0.62 0.486011 0.34
361 656 1w3b (A) 70 328 28.00 0.00000000072 1 0.587306 0.73
479 538 2c2l (A) 68 127 35.00 0.17 0.99 0.572272 -0.4
574 645 4gyw (A) 314 385 29.00 0 1 0.620926 -1.37
588 643 1elw (A) 15 70 29.00 0.22 1 0.55722 -0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_130245)

Species Accession Gene Product
Caenorhabditis elegans CELE_T25F10.5   Protein BBS-8
Drosophila melanogaster Dmel_CG13691   Bardet-Biedl syndrome 8 ortholog (H. sapiens)
Giardia lamblia GL50803_8508   Basal body protein
Homo sapiens ENSG00000165533   tetratricopeptide repeat domain 8
Leishmania braziliensis LbrM.09.0450   hypothetical tetratricopeptide repeat protein
Leishmania donovani LdBPK_090490.1   Bardet-Biedl syndrome 8 protein
Leishmania infantum LinJ.09.0490   hypothetical tetratricopeptide repeat protein
Leishmania major LmjF.09.0440   hypothetical tetratricopeptide repeat protein
Leishmania mexicana LmxM.09.0440   hypothetical tetratricopeptide repeat protein
Loa Loa (eye worm) LOAG_08914   hypothetical protein
Loa Loa (eye worm) LOAG_01237   hypothetical protein
Mus musculus ENSMUSG00000021013   tetratricopeptide repeat domain 8
Schistosoma japonicum Sjp_0113970   Tetratricopeptide repeat protein 8, putative
Schistosoma mansoni Smp_160860   tetratricopeptide repeat protein 8 tpr8
Schmidtea mediterranea mk4.006700.02  
Schmidtea mediterranea mk4.002757.00   Tetratricopeptide repeat protein 8
Trypanosoma brucei gambiense Tbg972.11.13920   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.12450   Bardet-Biedl syndrome 8 protein
Trypanosoma congolense TcIL3000_0_24890   Bardet-Biedl syndrome 8 protein
Trypanosoma congolense TcIL3000_0_20320   Tetratricopeptide repeat/TPR repeat, putative
Trypanosoma cruzi TcCLB.503657.20   Bardet-Biedl syndrome 8 protein
Trypanosoma cruzi TcCLB.511127.360   tetratricopeptide repeat protein 8
Trichomonas vaginalis TVAG_337680   tetratricopeptide repeat protein 8, tpr8, putative

Essentiality

TcCLB.511127.360 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.4290 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.4290 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.4290 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.4290 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511127.360 (Trypanosoma cruzi), tetratricopeptide repeat protein 8
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