Detailed view for NCLIV_047220

Basic information

TDR Targets ID: 475951
Neospora caninum, Calcium-dependent protein kinase, related
Source Database / ID: 

pI: 6.9891 | Length (AA): 716 | MW (Da): 79214 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00686   Starch binding domain
PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:2001070   GO:starch binding  

GO:0030246   carbohydrate binding  
GO:0005524   ATP binding  
GO:0005509   calcium ion binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

Plant-pathogen interaction (KEGG)
Toxoplasmosis (KEGG)

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 103 1pam (A) 585 684 21.00 0 0.79 0.353255 -0.71
2 103 1cgt (A) 584 683 20.00 0 1 0.339858 -0.59
13 105 2vn4 (A) 506 599 32.00 0 0.99 0.385288 -0.16
16 101 2z0b (A) 21 115 24.00 0.004 0.56 0.269712 0.24
116 493 3c4w (B) 64 441 26.00 0 1 0.788533 -0.03
225 684 4rgj (A) 45 527 36.00 0 1 1.06506 -0.45
233 684 3lij (A) 50 514 38.00 0 1 1.13128 -0.73
233 681 3hx4 (A) 39 503 37.00 0 1 1.11169 -0.67
240 511 3f3z (A) 23 294 67.00 0 1 1.26249 -1.35
243 658 5iso (C) 14 542 26.00 0 1 0.793606 0.65
543 607 5d67 (A) 1024 1087 22.00 0.67 0.91 0.534382 -1.65
617 683 2m97 (A) 1 69 30.00 0.000000000084 1 0.568175 -1.17
619 679 5i0i (I) 83 145 45.00 0.0000059 1 0.543196 0.19
622 716 5vln (A) 21 164 34.00 0.046 0.97 0.399682 0.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_170347)

Species Accession Gene Product
Cryptosporidium hominis Chro.70214   calmodulin-domain protein kinase 2
Cryptosporidium parvum cgd7_1840   calcium/calmodulin-dependent protein kinase with a kinase domain and 4 calmodulin like EF hands
Neospora caninum NCLIV_047220   Calcium-dependent protein kinase, related
Toxoplasma gondii TGME49_225490   calcium-dependent protein kinase CDPK2

Essentiality

NCLIV_047220 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_225490 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus MAP kinase p38 alpha 360 aa 29.0% 300 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 32.0% 291 aa Compounds References
Zea mays CaM kinase I alpha 492 aa 40.3% 457 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 28.9% 298 aa Compounds References
Plasmodium falciparum (isolate 3D7) Calcium-dependent protein kinase 4 528 aa 35.5% 516 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 22.8% 312 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 29.9% 291 aa Compounds References
Triticum aestivum Calcium dependent protein kinase 548 aa 38.6% 451 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 32.7% 294 aa Compounds References
Bos taurus Calmodulin 149 aa 36.7% 147 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 28.9% 308 aa Compounds References
Patiria pectinifera Cdc2 300 aa 30.0% 287 aa Compounds References
Rattus norvegicus Protein S100-B 92 aa 30.7% 75 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier NCLIV_047220 (Neospora caninum), Calcium-dependent protein kinase, related
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