Detailed view for PF3D7_0934500

Basic information

TDR Targets ID: 5015
Plasmodium falciparum, V-type proton ATPase subunit E, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.5577 | Length (AA): 235 | MW (Da): 27191 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01991   ATP synthase (E/31 kDa) subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0046961   hydrogen ion transporting ATPase activity, rotational mechanism  
GO:0033178   proton-transporting two-sector ATPase complex, catalytic domain  
GO:0015986   ATP synthesis coupled proton transport  
GO:0015991   ATP hydrolysis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 235 4efa (E) 7 226 31.00 0 1 1.31664 0.09
4 175 4uos (A) 1 183 18.00 0.00021 0.12 0.944615 -1.21

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Oocyst, Ring, Sporozoite, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile gametocyte, Female Gametocyte. PlasmoDB Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127015)

Species Accession Gene Product
Arabidopsis thaliana AT4G11150   V-type proton ATPase subunit E1
Arabidopsis thaliana AT1G64200   V-type proton ATPase subunit E3
Arabidopsis thaliana AT3G08560   V-type proton ATPase subunit E2
Babesia bovis BBOV_I000090   ATP synthase subunit E containing protein
Brugia malayi Bm1_53000   Vacuolar h atpase protein 8
Candida albicans CaO19.10129   vacuolar ATPase V1 domain subunit E similar to S. cerevisiae VMA4 (YOR332W)
Candida albicans CaO19.2598   vacuolar ATPase V1 domain subunit E similar to S. cerevisiae VMA4 (YOR332W)
Caenorhabditis elegans CELE_C17H12.14   Protein VHA-8
Cryptosporidium hominis Chro.80048   vacuolar ATP synthase subunit E
Cryptosporidium parvum cgd8_360   putative vacuolar ATP synthase subunit E
Dictyostelium discoideum DDB_G0275701   vacuolar H+-ATPase E subunit
Drosophila melanogaster Dmel_CG1088   Vacuolar H[+]-ATPase 26kD subunit
Echinococcus granulosus EgrG_001118800   Vacuolar proton pump subunit E
Entamoeba histolytica EHI_182560   Vacuolar ATP synthase subunit E, putative
Entamoeba histolytica EHI_182550   Vacuolar ATP synthase subunit E, putative
Echinococcus multilocularis EmuJ_001118800   Vacuolar proton pump subunit E
Giardia lamblia GL50803_13603   Hypothetical protein
Homo sapiens ENSG00000131100   ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Homo sapiens ENSG00000250565   ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
Leishmania braziliensis LbrM.35.3320   ATP synthase, putative
Leishmania donovani LdBPK_363250.1   ATP synthase, putative
Leishmania infantum LinJ.36.3250   ATP synthase, putative
Leishmania major LmjF.36.3100   ATP synthase, putative
Leishmania mexicana LmxM.36.3100   ATP synthase, putative
Loa Loa (eye worm) LOAG_00780   vacuolar h ATPase 8
Mus musculus ENSMUSG00000019210   ATPase, H+ transporting, lysosomal V1 subunit E1
Mus musculus ENSMUSG00000053375   ATPase, H+ transporting, lysosomal V1 subunit E2
Neospora caninum NCLIV_043880   hypothetical protein
Oryza sativa 4326699   Os01g0222500
Oryza sativa 4339122   Os05g0480700
Oryza sativa 4327166   Os01g0659200
Plasmodium berghei PBANKA_0835300   V-type proton ATPase subunit E, putative
Plasmodium falciparum PF3D7_0934500   V-type proton ATPase subunit E, putative
Plasmodium knowlesi PKNH_0733200   V-type proton ATPase subunit E, putative
Plasmodium vivax PVX_086990   vacuolar ATP synthase subunit e, putative
Plasmodium yoelii PY06101   ATP synthase subunit
Saccharomyces cerevisiae YOR332W   H(+)-transporting V1 sector ATPase subunit E
Schistosoma japonicum Sjp_0209790   ko:K02150 V-type H+-transporting ATPase subunit E, putative
Schistosoma mansoni Smp_085520   vacuolar ATP synthase subunit E
Schmidtea mediterranea mk4.002005.01  
Schmidtea mediterranea mk4.001357.03   V-type proton ATPase subunit E
Trypanosoma brucei gambiense Tbg972.11.10570   ATP synthase, putative
Trypanosoma brucei Tb927.11.9420   ATP synthase, putative
Trypanosoma congolense TcIL3000.11.9840   ATP synthase, putative
Trypanosoma cruzi TcCLB.511589.10   ATP synthase, putative
Trypanosoma cruzi TcCLB.508851.59   ATP synthase, putative
Toxoplasma gondii TGME49_305290   vacuolar atp synthase subunit e, putative
Theileria parva TP03_0853   vacuolar ATP synthase subunit E, putative
Theileria parva TP03_0247   hypothetical protein
Trichomonas vaginalis TVAG_360810   vacuolar ATP synthase subunit E, putative
Trichomonas vaginalis TVAG_448420   vacuolar ATP synthase subunit E, putative
Trichomonas vaginalis TVAG_021890   vacuolar ATP synthase subunit E, putative
Trichomonas vaginalis TVAG_099990   vacuolar ATP synthase subunit E, putative

Essentiality

PF3D7_0934500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1190 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1190 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.1190 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.1190 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C17H12.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_C17H12.14 Caenorhabditis elegans larval arrest wormbase
CELE_C17H12.14 Caenorhabditis elegans larval lethal wormbase
CELE_C17H12.14 Caenorhabditis elegans slow growth wormbase
CELE_C17H12.14 Caenorhabditis elegans sterile wormbase
PBANKA_0835300 Plasmodium berghei Essential plasmo
TGME49_305290 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

11 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0934500 (Plasmodium falciparum), V-type proton ATPase subunit E, putative
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