Detailed view for Tbg972.8.1930

Basic information

TDR Targets ID: 543999
Trypanosoma brucei gambiense, 3-oxo-5-alpha-steroid 4-dehydrogenase-like, putative
Source Database / ID: 

pI: 8.9097 | Length (AA): 335 | MW (Da): 36939 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 6

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02544   3-oxo-5-alpha-steroid 4-dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016627   oxidoreductase activity, acting on the CH-CH group of donors  
GO:0016021   integral to membrane  
GO:0005737   cytoplasm  
GO:0006629   lipid metabolic process  

Metabolic Pathways

Steroid hormone biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
34 170 1pjc (A) 204 327 32.00 0.33 0.74 0.444555 2.22
215 301 2ns1 (A) 152 237 37.00 0.67 0.25 0.168301 2.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128852)

Species Accession Gene Product
Arabidopsis thaliana AT2G38050   probable steroid reductase DET2
Brugia malayi Bm1_06570   3-oxo-5-alpha-steroid 4-dehydrogenase 1
Caenorhabditis elegans CELE_F42F12.3   Protein F42F12.3
Caenorhabditis elegans CELE_F19H6.4   Protein F19H6.4
Entamoeba histolytica EHI_197070   steroid 5-alpha reductase, putative
Homo sapiens ENSG00000145545   steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Homo sapiens 6716   steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
Leishmania braziliensis LbrM.17.1210   3-oxo-5-alpha-steroid 4-dehydrogenase-like protein
Leishmania donovani LdBPK_171200.1   3-oxo-5-alpha-steroid 4-dehydrogenase-like protein
Leishmania infantum LinJ.17.1200   3-oxo-5-alpha-steroid 4-dehydrogenase-like protein
Leishmania major LmjF.17.1100   3-oxo-5-alpha-steroid 4-dehydrogenase-like protein
Leishmania mexicana LmxM.17.1100   3-oxo-5-alpha-steroid 4-dehydrogenase-like protein
Loa Loa (eye worm) LOAG_07203   hypothetical protein
Mus musculus ENSMUSG00000021594   steroid 5 alpha-reductase 1
Mus musculus ENSMUSG00000038541   steroid 5 alpha-reductase 2
Mycobacterium ulcerans MUL_3208   hypothetical protein
Oryza sativa 9272051   Os11g0184100
Oryza sativa 4324809   Os01g0851600
Schmidtea mediterranea mk4.000630.02   3-oxo-5-alpha-steroid 4-dehydrogenase 1
Schmidtea mediterranea mk4.000630.01  
Trypanosoma brucei gambiense Tbg972.8.1930   3-oxo-5-alpha-steroid 4-dehydrogenase-like, putative
Trypanosoma brucei Tb927.8.2480   3-oxo-5-alpha-steroid 4-dehydrogenase-like, putative
Trypanosoma cruzi TcCLB.509331.70   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trypanosoma cruzi TcCLB.504427.70   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Toxoplasma gondii TGME49_304480   3-oxo-5-alpha-steroid 4-dehydrogenase
Trichomonas vaginalis TVAG_146690   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trichomonas vaginalis TVAG_391520   3-oxo-5-alpha-steroid 4-dehydrogenase, putative
Trichomonas vaginalis TVAG_096490   3-oxo-5-alpha-steroid 4-dehydrogenase, putative

Essentiality

Tbg972.8.1930 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2480 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2480 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.2480 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F19H6.4 Caenorhabditis elegans embryonic lethal wormbase
TGME49_304480 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Steroid 5-alpha-reductase 1 Compounds References
Homo sapiens steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) Compounds References
Rattus norvegicus Steroid 5-alpha-reductase 2 Compounds References
Homo sapiens steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Steroid 5-alpha-reductase 254 aa 29.7% 283 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tbg972.8.1930 (Trypanosoma brucei gambiense), 3-oxo-5-alpha-steroid 4-dehydrogenase-like, putative
Title for this comment
Comment