Detailed view for PF3D7_0811300

Basic information

TDR Targets ID: 625
Plasmodium falciparum, CCR4-associated factor 1
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.4073 | Length (AA): 1774 | MW (Da): 200212 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005634   nucleus  
GO:0003676   nucleic acid binding  

Metabolic Pathways

RNA degradation (KEGG)

Structural information

Modbase 3D models:

There are 18 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 258 1uoc (A) 12 286 37.00 0 1 0.56 -2.02
6 268 4gmj (B) 11 280 56.00 0 1 0.852453 -1.5
7 257 2p51 (A) 22 271 49.00 0 1 0.850388 -1.65
9 331 3d45 (A) 2 493 19.00 0.000000000044 1 0.245274 0.01
521 756 4r6f (A) 46 302 30.00 0.22 0.75 0.233933 0.48
525 1019 4mn8 (A) 319 774 24.00 0.029 0.85 0.20093 0.97
911 1146 3n7l (A) 920 1158 29.00 0.88 0.8 0.166933 0.7
1060 1230 4b60 (A) 246 448 32.00 0.018 0.11 0.221292 -0.3
1235 1341 3ult (A) 6 114 27.00 0.00016 0.49 0.618216 -2.65
1361 1771 1s0f (A) 743 1185 25.00 0.97 0.86 0.11858 1.13
6 267 4gmj (B) 11 279 56.00 0 1 0.852239 -1.56
7 257 2p51 (A) 22 271 49.00 0 1 0.843328 -1.56
9 336 3d45 (B) 2 502 18.00 0.0045 1 0.307106 -0.2
1031 1641 3h09 (A) 332 963 23.00 0.00000096 0.69 0.434603 0.65
1061 1231 4b60 (A) 246 448 32.00 0.016 0.03 0.210156 -0.25
1223 1338 3ult (A) 6 114 28.00 0.00019 0.12 0.5341 -2.51
1291 1744 3s5m (A) 137 630 24.00 0.36 0.87 0.313952 0.75
1417 1686 4u0r (A) 161 493 15.00 0.85 0 0.259356 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, early ring, Oocyst, Ring, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile early schizont, early trophozoite, late ring, late schizont, late trophozoite, Female Gametocyte, Male Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile gametocyte. PlasmoDB
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_127146)

Species Accession Gene Product
Arabidopsis thaliana AT2G32070   polynucleotidyl transferase, ribonuclease H-like superfamily protein
Arabidopsis thaliana AT1G80780   putative CCR4-associated factor 1-like 6
Arabidopsis thaliana AT5G10960   polynucleotidyl transferase, ribonuclease H-like superfamily protein
Babesia bovis BBOV_III006830   CAF1 family ribonuclease containing protein
Brugia malayi Bm1_07025   CCR4-NOT transcription complex subunit 7
Candida albicans CaO19.5734   potential mRNA deadenylase similar to S. cerevisiae POP2/CAF1 (YNR052C) ribonuclease-like component of the cytoplasmic mRNA dead
Candida albicans CaO19.13156   potential mRNA deadenylase similar to S. cerevisiae POP2/CAF1 (YNR052C) ribonuclease-like component of the cytoplasmic mRNA dead
Caenorhabditis elegans CELE_Y56A3A.20   Protein CCF-1
Cryptosporidium hominis Chro.30052   hypothetical protein
Cryptosporidium parvum cgd3_350   Pop2p-like 35 exonuclease, CCR4-NOT transcription complex
Dictyostelium discoideum DDB_G0284217   CAF1 family protein
Dictyostelium discoideum DDB_G0285269   CAF1 family protein
Drosophila melanogaster Dmel_CG5684   CG5684 gene product from transcript CG5684-RA
Echinococcus granulosus EgrG_001187400   CCR4 NOT transcription complex subunit 7
Entamoeba histolytica EHI_039000   CAF1 family ribonuclease, putative
Entamoeba histolytica EHI_048150   CAF1 family ribonuclease, putative
Echinococcus multilocularis EmuJ_001187400   CCR4 NOT transcription complex subunit 7
Giardia lamblia GL50803_10606   CCR4-NOT transcription complex, subunit 7
Giardia lamblia GL50803_8209   CCR4-NOT transcription complex, subunit 7
Homo sapiens ENSG00000198791   CCR4-NOT transcription complex, subunit 7
Homo sapiens ENSG00000155508   CCR4-NOT transcription complex, subunit 8
Leishmania braziliensis LbrM.22.1540   CCR4 associated factor, putative
Leishmania donovani LdBPK_221480.1   ccr4-not transcription complex subunit
Leishmania infantum LinJ.22.1480   CCR4 associated factor, putative
Leishmania major LmjF.22.1630   CCR4 associated factor, putative
Leishmania mexicana LmxM.22.1630   CCR4 associated factor, putative
Loa Loa (eye worm) LOAG_04270   CCR4-NOT transcription complex subunit 7
Mus musculus ENSMUSG00000020515   CCR4-NOT transcription complex, subunit 8
Mus musculus ENSMUSG00000031601   CCR4-NOT transcription complex, subunit 7
Neospora caninum NCLIV_026760   CCR4-NOT transcription complex,subunit 7-like, related
Oryza sativa 4347066   Os09g0416800
Oryza sativa 4331006   Os02g0796300
Plasmodium berghei PBANKA_1426200   CCR4-associated factor 1, putative
Plasmodium falciparum PF3D7_0811300   CCR4-associated factor 1
Plasmodium knowlesi PKNH_1429400   CCR4-associated factor 1, putative
Plasmodium vivax PVX_123205   CCR4-associated factor 1, putative
Plasmodium yoelii PY04679   hypothetical protein
Plasmodium yoelii PY01168   ccr4-not transcription complex, subunit 7
Saccharomyces cerevisiae YNR052C   Pop2p
Schistosoma japonicum Sjp_0103700   CCR4-NOT transcription complex subunit 7, putative
Schistosoma mansoni Smp_021870   ccr4-associated factor
Schmidtea mediterranea mk4.003641.00   CCR4-NOT transcription complex subunit 7
Schmidtea mediterranea mk4.001247.20   CCR4-NOT transcription complex subunit 7
Schmidtea mediterranea mk4.002031.07   CCR4-NOT transcription complex subunit 7
Schmidtea mediterranea mk4.000784.10   CCR4-NOT transcription complex subunit 7
Trypanosoma brucei gambiense Tbg972.6.270   CCR4 associated factor, putative
Trypanosoma brucei Tb927.6.600   ccr4-not transcription complex subunit
Trypanosoma congolense TcIL3000_6_120   ccr4-not transcription complex subunit
Trypanosoma cruzi TcCLB.510535.60   CCR4-associated factor 1
Trypanosoma cruzi TcCLB.511827.60   ccr4-not transcription complex subunit
Toxoplasma gondii TGME49_260240   ccr4-associated factor family protein
Theileria parva TP04_0580   hypothetical protein
Trichomonas vaginalis TVAG_149020   CCR4-associated factor, putative
Trichomonas vaginalis TVAG_007620   CCR4-associated factor, putative

Essentiality

PF3D7_0811300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.600 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.600 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.600 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.600 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y56A3A.20 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y56A3A.20 Caenorhabditis elegans larval arrest wormbase
CELE_Y56A3A.20 Caenorhabditis elegans larval lethal wormbase
CELE_Y56A3A.20 Caenorhabditis elegans slow growth wormbase
CELE_Y56A3A.20 Caenorhabditis elegans sterile wormbase
YNR052C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1426200 Plasmodium berghei Essential plasmo
TGME49_260240 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens CCR4-NOT transcription complex, subunit 7 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0137 0.8593 1
0.0157 0.487 0.5
0.0149 0.6272 1
0.018 0.8472 1
0.0164 0.8472 1
0.0125 0.8472 1
0.0163 0.3943 1
0.0157 0.487 0.5
0.0162 0.8472 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0811300 (Plasmodium falciparum), CCR4-associated factor 1
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