Detailed view for Rv0866

Basic information

TDR Targets ID: 6506
Mycobacterium tuberculosis, Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT]
Source Database / ID:  Tuberculist 

pI: 6.1318 | Length (AA): 141 | MW (Da): 15048 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02391   MoaE protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0006777   Mo-molybdopterin cofactor biosynthetic process  

Metabolic Pathways

Folate biosynthesis (KEGG)
Global map (KEGG)
Sulfur relay system (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 139 2q5w (E) 2 136 29.00 0 0.96 1.33064 0.12
6 140 1fm0 (E) 5 139 30.00 0 1 1.22355 0.32
6 130 2wp4 (A) 12 136 40.00 0 1 1.26362 0.31
9 131 5mpo (C) 49 171 31.00 0 1 1.24744 0.2

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2WP4:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_128296)

Species Accession Gene Product
Arabidopsis thaliana AT2G43760   Molybdopterin synthase catalytic subunit
Caenorhabditis elegans CELE_T27A3.6   Protein T27A3.6
Dictyostelium discoideum DDB_G0271864   molybdenum cofactor synthesis protein 2 large subunit
Drosophila melanogaster Dmel_CG10238   Molybdenum cofactor synthesis 2
Escherichia coli b0785   molybdopterin synthase, large subunit
Homo sapiens ENSG00000164172   molybdenum cofactor synthesis 2
Loa Loa (eye worm) LOAG_00832   hypothetical protein
Mus musculus ENSMUSG00000015536   molybdenum cofactor synthesis 2
Mycobacterium tuberculosis Rv3119   Probable molybdenum cofactor biosynthesis protein E MoaE1 (molybdopterin converting factor large subunit) (molybdopterin [MPT] c
Mycobacterium tuberculosis Rv0866   Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT]
Mycobacterium tuberculosis Rv3323c   Probable MoaD-MoaE fusion protein MoaX
Mycobacterium ulcerans MUL_0284   molybdenum cofactor biosynthesis protein E2 MoaE2
Oryza sativa 4328260   Os02g0140300
Onchocerca volvulus OVOC254   Molybdopterin synthase catalytic subunit homolog
Schistosoma japonicum Sjp_0306770   ko:K03635 molybdenum cofactor biosynthesis protein E, putative
Schmidtea mediterranea mk4.019893.00   Molybdopterin synthase catalytic subunit
Schmidtea mediterranea mk4.001953.02   Molybdopterin synthase catalytic subunit
Toxoplasma gondii TGME49_273350   molybdopterin converting factor, subunit 2 protein

Essentiality

Rv0866 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu882 this record Mycobacterium tuberculosis non-essential nmpdr
mtu3383 Mycobacterium tuberculosis non-essential nmpdr
b0785 Escherichia coli non-essential goodall
TGME49_273350 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0866 (Mycobacterium tuberculosis), Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT]
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