Detailed view for Rv0357c

Basic information

TDR Targets ID: 6955
Mycobacterium tuberculosis, Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase)

Source Database / ID: Tuberculist / Rv0357c 

pI: 6.6927 | Length (AA): 432 | MW (Da): 46823 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Pfam domains

PF00709   Adenylosuccinate synthetase

Gene Ontology

Mouse over links to read term descriptions.
GO:0000287   magnesium ion binding  
GO:0005525   GTP binding  
GO:0004019   adenylosuccinate synthase activity  
GO:0006164   purine nucleotide biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 429 4m0g (B) 1 428 50.00 0 1 1.63416 -0.78
4 423 1iwe (A) 34 455 44.00 0 1 1.52462 -0.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the lower 20-40% group of genes. Dormant phase.
Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the lower 0-20% group of genes. Dormant phase.
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127011)

Species Accession Gene Product
Arabidopsis thaliana AT3G57610   adenylosuccinate synthetase
Babesia bovis BBOV_II004950   adenylosuccinate synthetase, putative
Brugia malayi Bm1_50695   adss-prov protein
Candida albicans CaO19.12290   Adenylosuccinate synthetase
Candida albicans CaO19.4827   Adenylosuccinate synthetase
Caenorhabditis elegans CELE_C37H5.6   Protein C37H5.6, isoform B
Dictyostelium discoideum DDB_G0285429   adenylosuccinate synthetase
Drosophila melanogaster Dmel_CG17273   Adenylosuccinate Synthetase
Escherichia coli b4177   adenylosuccinate synthetase
Echinococcus granulosus EgrG_001056900   adenylosuccinate synthetase
Echinococcus multilocularis EmuJ_001056900   adenylosuccinate synthetase
Homo sapiens ENSG00000035687   adenylosuccinate synthase
Homo sapiens ENSG00000185100   adenylosuccinate synthase like 1
Leishmania braziliensis LbrM.13.0970   adenylosuccinate synthetase, putative
Leishmania donovani LdBPK_131090.1   adenylosuccinate synthetase, putative
Leishmania infantum LinJ.13.1090   adenylosuccinate synthetase, putative
Leishmania major LmjF.13.1190   adenylosuccinate synthetase, putative
Leishmania mexicana LmxM.13.1190   adenylosuccinate synthetase, putative
Loa Loa (eye worm) LOAG_01402   adss-prov protein
Mycobacterium leprae ML0280c   Probable adenylosuccinate synthase PurA (IMP--aspartate ligase) (AdSS) (AMPSase)
Mus musculus ENSMUSG00000011148   adenylosuccinate synthetase like 1
Mus musculus ENSMUSG00000015961   adenylosuccinate synthetase, non muscle
Mycobacterium tuberculosis Rv0357c this record   Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase)
Mycobacterium ulcerans MUL_2834   adenylosuccinate synthetase
Neospora caninum NCLIV_038330   adenylosuccinate synthetase, putative
Oryza sativa 4331792   Os03g0174500
Oryza sativa 4333815   Os03g0699300
Onchocerca volvulus OVOC7126   Adenylosuccinate synthetase homolog
Plasmodium berghei PBANKA_1131100   adenylosuccinate synthetase, putative
Plasmodium falciparum PF3D7_1354500   adenylosuccinate synthetase
Plasmodium knowlesi PKNH_1117300   adenylosuccinate synthetase, putative
Plasmodium vivax PVX_114710   adenylosuccinate synthetase, putative
Plasmodium yoelii PY03301   adenylosuccinate synthetase
Saccharomyces cerevisiae YNL220W   adenylosuccinate synthase
Schistosoma japonicum Sjp_0015930   ko:K01939 adenylosuccinate synthase [EC6.3.4.4], putative
Schistosoma mansoni Smp_055990   adenylosuccinate synthetase
Schmidtea mediterranea mk4.028749.00   Adenylosuccinate synthetase
Schmidtea mediterranea mk4.033749.02   Adenylosuccinate synthetase
Schmidtea mediterranea mk4.014784.00  
Schmidtea mediterranea mk4.031257.00   Adenylosuccinate synthetase
Trypanosoma brucei gambiense Tbg972.11.4160   adenylosuccinate synthetase, putative
Trypanosoma brucei Tb11.v5.0435   adenylosuccinate synthetase, putative
Trypanosoma congolense TcIL3000.11.3610   adenylosuccinate synthetase, putative
Trypanosoma cruzi TcCLB.509683.80   adenylosuccinate synthetase, putative
Trypanosoma cruzi TcCLB.508731.60   adenylosuccinate synthetase, putative
Toxoplasma gondii TGME49_279450   adenylosuccinate synthetase, putative
Theileria parva TP04_0033   adenylosuccinate synthase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0273   adenylosuccinate synthetase

Essentiality

Rv0357c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu360 this record Mycobacterium tuberculosis essential nmpdr
Tb11.02.1120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.1120 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.1120 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b4177 Escherichia coli non-essential goodall
CELE_C37H5.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_C37H5.6 Caenorhabditis elegans slow growth wormbase
TGME49_279450 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens adenylosuccinate synthase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv0357c (Mycobacterium tuberculosis), Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase)
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