Detailed view for EHI_023110

Basic information

TDR Targets ID: 800056
Entamoeba histolytica, NADP-dependent alcohol dehydrogenase
Source Database / ID:  AmoebaDB  

pI: 6.5544 | Length (AA): 366 | MW (Da): 39215 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00107   Zinc-binding dehydrogenase
PF08240   Alcohol dehydrogenase GroES-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0008270   zinc ion binding  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 366 2oui (A) 1 360 99.99 0 1 2.19881 -1.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1Y9A:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2OUI:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3FPC:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_126928)

Species Accession Gene Product
Arabidopsis thaliana AT5G51970   putative sorbitol dehydrogenase
Candida albicans CaO19.769   likely Zinc-binding dehydrogenase, one of two genes similar to S. cerevisiae YAL061W and BDH1 (YAL060W) butanediol dehydrogenase
Candida albicans CaO19.7676   similar to P.stipitis xylitol dehydrogenase (XYL2), and to S. cerevisiae SOR1 (YJR159W) sorbitol dehydrogenase
Caenorhabditis elegans CELE_R04B5.6   Protein R04B5.6
Caenorhabditis elegans CELE_R04B5.5   Protein R04B5.5
Drosophila melanogaster Dmel_CG1982   Sorbitol dehydrogenase 1
Drosophila melanogaster Dmel_CG4649   Sorbitol dehydrogenase-2
Escherichia coli b1774   putative Zn-dependent NAD(P)-binding oxidoreductase
Escherichia coli b2091   galactitol-1-phosphate dehydrogenase, Zn-dependent and NAD(P)-binding
Escherichia coli b3616   L-threonine 3-dehydrogenase, NAD(P)-binding
Escherichia coli b4267   L-idonate 5-dehydrogenase, NAD-binding
Escherichia coli b1776   putative Zn-dependent NAD(P)-binding oxidoreductase
Entamoeba histolytica EHI_023110   NADP-dependent alcohol dehydrogenase
Homo sapiens 6652   sorbitol dehydrogenase
Leishmania braziliensis LbrM.33.0520   d-xylulose reductase, putative
Leishmania donovani LdBPK_330530.1   d-xylulose reductase, putative
Leishmania infantum LinJ.33.0530   d-xylulose reductase, putative
Leishmania major LmjF.33.0520   d-xylulose reductase, putative
Leishmania mexicana LmxM.32.0520   d-xylulose reductase, putative
Loa Loa (eye worm) LOAG_12517   hypothetical protein
Mus musculus ENSMUSG00000027227   sorbitol dehydrogenase
Mycobacterium ulcerans MUL_0819   short-chain dehydrogenase
Oryza sativa 4346217   Os08g0545200
Saccharomyces cerevisiae YAL061W   putative dehydrogenase BDH2
Saccharomyces cerevisiae YJR159W   L-iditol 2-dehydrogenase SOR1
Saccharomyces cerevisiae YAL060W   (R,R)-butanediol dehydrogenase
Saccharomyces cerevisiae YDL246C   L-iditol 2-dehydrogenase SOR2
Saccharomyces cerevisiae YLR070C   D-xylulose reductase XYL2
Schmidtea mediterranea mk4.002176.02  
Schmidtea mediterranea mk4.002176.01  

Essentiality

EHI_023110 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1774 Escherichia coli non-essential goodall
b1776 Escherichia coli non-essential goodall
b2091 Escherichia coli non-essential goodall
b3616 Escherichia coli non-essential goodall
b4267 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Sorbitol dehydrogenase Compounds References
Homo sapiens sorbitol dehydrogenase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0067 0.4566 1
0.0613 0.4221 1
0.0126 0.4454 0.4288
0.0067 0.6675 1
0.0232 0.8761 1
0.0613 0.4221 1
0.0664 0.8299 0.813
0.0067 0.3009 0.8768
0.0664 0.8299 0.813
0.0067 0.2709 0.2709
0.0242 0.3416 1
0.0221 0.2559 0.2483
0.0664 0.8299 0.813
0.0244 0.5462 1
0.0384289 0.366107 1
0.0664 0.8299 0.813
0.0505 0.4106 0.7742
0.0242 0.3416 1
0.0186 0.6864 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_023110 (Entamoeba histolytica), NADP-dependent alcohol dehydrogenase
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