Detailed view for LmxM.27.0500

Basic information

TDR Targets ID: 824470
Leishmania mexicana, calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Source Database / ID: 

pI: 4.9404 | Length (AA): 5753 | MW (Da): 654113 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease
PF01067   Calpain large subunit, domain III

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 22 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
188 737 1df0 (A) 22 598 19.00 0 1 0.0453023 1.04
213 501 2r9f (A) 51 349 21.00 0 1 0.201935 -0.61
262 471 2nqa (A) 99 305 33.00 0.00000035 1 0.340403 0.48
795 929 5szh (A) 532 671 11.00 0.64 0.01 0.150166 -0.42
1496 2474 5hdt (A) 39 1070 14.00 0 1 0.189872 0.51
1789 2664 4knh (B) 18 956 14.00 0 0.93 0.185968 0.49
1933 2079 5nnv (D) 267 854 29.00 0.47 0.31 0.183452 0.35
2116 3231 3s4w (B) 114 1358 12.00 0 0.8 0.158686 0.66
2298 3162 1jqn (A) 29 862 17.00 0 0.98 0.0870563 0.82
2303 3205 1jqo (A) 45 955 16.00 0 1 0.141662 0.66
2915 3271 5h7c (A) 31 347 22.00 0.44 1 0.181955 0.02
2918 3151 5cwp (A) 1 229 17.00 0 0.17 0.233374 -0.87
2918 3292 5h7c (A) 16 394 16.00 0 0.6 0.214883 -0.32
3074 3287 4tql (A) 22 238 28.00 0.042 1 0.214098 -0.45
3930 4170 5j1g (A) 1004 1228 13.00 0 0.17 0.133591 -0.94
4144 5136 1st6 (A) 7 965 13.00 0 0.89 0.151306 0.61
4666 4902 3s84 (A) 99 328 27.00 0.29 0.74 0.243096 -0.06
4873 5046 4uos (A) 1 176 14.00 0.14 0.08 0.294945 -1.53
5080 5552 1qxp (A) 7 516 21.00 0 1 0.156918 0.26
5090 5629 3bow (A) 23 589 19.00 0 0.96 0.120564 0.4
5092 5403 1zcm (A) 35 353 21.00 0 1 0.201933 -0.57
5412 5550 2qfe (A) 693 812 31.00 0.033 0.25 0.134861 -0.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130635)

Species Accession Gene Product
Leishmania braziliensis LbrM.27.0610   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.27.0620   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.27.2140   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.27.0600   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania donovani LdBPK_270510.1   calpain-like cysteine peptidase, putative
Leishmania infantum LinJ.27.0500   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.27.2520   cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.27.2490   calpain-like cysteine peptidase
Leishmania infantum LinJ.27.0510   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.27.0490   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.27.0500   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.27.0510   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.27.0500   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.27.0490   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.27.0510   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.11.1180   calpain-like protein, putative
Trypanosoma brucei gambiense Tbg972.11.1190   calpain-like cysteine peptidase, putative,antigen, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.11.1170  
Trypanosoma brucei Tb11.v5.0432   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb927.11.1130   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb427tmp.11.0001   hypothetical protein
Trypanosoma brucei Tb11.v5.1010   variant surface glycoprotein (VSG), putative
Trypanosoma brucei Tb927.11.1090   calpain-like protein, putative
Trypanosoma brucei Tb11.v5.1011   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb11.v5.1038   calpain-like cysteine peptidase, putative
Trypanosoma brucei Tb927.11.1100   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.11.1110   calpain, putative
Trypanosoma brucei Tb11.v5.0702   calpain-like cysteine peptidase, putative
Trypanosoma congolense TcIL3000.11.1030   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma congolense TcIL3000_0_11260   calpain, putative
Trypanosoma cruzi TcCLB.511445.10   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.511441.10   calpain cysteine peptidase, putative
Trypanosoma cruzi TcCLB.509013.10   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.445281.9   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.506721.30   cysteine peptidase, Clan CA, family C2, putative

Essentiality

LmxM.27.0500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.v4.0001 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.v4.0001 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.v4.0001 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.v4.0001 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.47.0036 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.47.0035 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.47.0035 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.47.0035 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.47.0035 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.57.0008 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.57.0008 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.57.0008 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.57.0008 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)(HIV-1) Protein Rev Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
Human immunodeficiency virus 1 Human immunodeficiency virus type 1 REV Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmxM.27.0500 (Leishmania mexicana), calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
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