Detailed view for TGME49_209620

Basic information

TDR Targets ID: 260973
Toxoplasma gondii, eukaryotic aspartyl protease superfamily protein
Source Database / ID:  ToxoDB 

pI: 6.906 | Length (AA): 393 | MW (Da): 43489 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00026   Eukaryotic aspartyl protease

Gene Ontology

Mouse over links to read term descriptions.
GO:0004190   aspartic-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
35 392 3qvc (A) 94 310 20.00 0.89 1 0.977441 0.82
37 388 3psg (A) 26 300 28.00 0 1 1.06937 0.6
58 322 1qdm (A) 7 246 36.00 0 0.81 0.8614 0.78
58 321 1b5f (A) 3 237 33.00 0 1 0.899856 0.46
69 150 3q6y (A) 17 110 31.00 0.13 0.4 0.427751 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_150586)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG10104   CG10104 gene product from transcript CG10104-RA
Neospora caninum NCLIV_003910   Cathepsin D enzyme (EC 3.4.23.5), related
Plasmodium berghei PBANKA_1329100   plasmepsin VIII
Plasmodium falciparum PF3D7_1465700   plasmepsin VIII, putative
Plasmodium knowlesi PKNH_1215800   plasmepsin VIII, putative
Plasmodium vivax PVX_117180   aspartyl proteinase, putative
Plasmodium yoelii PY02004   Eukaryotic aspartyl protease
Toxoplasma gondii TGME49_209620   eukaryotic aspartyl protease superfamily protein

Essentiality

TGME49_209620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1329100 Plasmodium berghei Dispensable plasmo
TGME49_209620 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Cathepsin D 390 aa 25.1% 383 aa Compounds References
Oryctolagus cuniculus Renin 280 aa 27.0% 300 aa Compounds References
Sus scrofa Pepsin A 385 aa 25.3% 363 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0021 0.4249 0.5
0.0068 1 0.5
0.0021 1 0.5
0.0021 1 0.5
0.0021 0.443 0.5
0.0021 1 0.5
0.0021 0.7491 0.5
0.0021 1 0.5
0.0021 1 0.5
0.0106 0.5 0.5
0.0021 1 0.5
0.0106 0.5 0.5
0.002 0.5 0.5
0.0021 1 0.5
0.0021 1 0.5
0.0021 1 0.5

Assayability

Assay information

  • Assay for Pepsinogen (3.4.23.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

13 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_209620 (Toxoplasma gondii), eukaryotic aspartyl protease superfamily protein
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