Detailed view for CaO19.5142

Basic information

TDR Targets ID: 656807
Candida albicans, dihydrofolate reductase
Source Database / ID:  KEGG  

pI: 7.3165 | Length (AA): 192 | MW (Da): 22139 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00186   Dihydrofolate reductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004146   dihydrofolate reductase activity  
GO:0055114   oxidation reduction  
GO:0046654   tetrahydrofolate biosynthetic process  

Metabolic Pathways

One carbon pool by folate (KEGG)
Folate biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128410)

Species Accession Gene Product
Arabidopsis thaliana AT2G21550   bifunctional dihydrofolate reductase/thymidylate synthase
Brugia malayi Bm1_48330   Dihydrofolate reductase
Brugia malayi Bm1_54930   dihydrofolate reductase family protein
Candida albicans CaO19.12607   dihydrofolate reductase
Candida albicans CaO19_5142   hypothetical protein
Candida albicans CaO19.5142   dihydrofolate reductase
Caenorhabditis elegans CELE_C36B1.7   Protein DHFR-1
Chlamydia trachomatis CT_612   dihydrofolate reductase
Dictyostelium discoideum DDB_G0286755   dihydrofolate reductase
Drosophila melanogaster Dmel_CG14887   Dihydrofolate reductase
Escherichia coli b0048   dihydrofolate reductase
Echinococcus granulosus EgrG_000572400   dihydrofolate reductase
Echinococcus multilocularis EmuJ_000572400   dihydrofolate reductase
Homo sapiens ENSG00000228716   dihydrofolate reductase
Loa Loa (eye worm) LOAG_03468   dihydrofolate reductase
Mycobacterium leprae ML1518c   DIHYDROFOLATE REDUCTASE DFRA (DHFR) (TETRAHYDROFOLATE DEHYDROGENASE)
Mus musculus ENSMUSG00000021707   dihydrofolate reductase
Mycobacterium tuberculosis Rv2763c   Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase)
Mycobacterium ulcerans MUL_2179   dihydrofolate reductase DfrA
Saccharomyces cerevisiae YOR236W   dihydrofolate reductase
Schistosoma japonicum Sjp_0071810   ko:K00287 dihydrofolate reductase [EC1.5.1.3], putative
Schistosoma mansoni Smp_175230   dihydrofolate reductase
Schmidtea mediterranea mk4.001198.04   Dihydrofolate reductase

Essentiality

CaO19.5142 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0048 Escherichia coli essential goodall
CELE_C36B1.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_C36B1.7 Caenorhabditis elegans sterile wormbase
YOR236W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

202 chemical compounds are associated with this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Dihydrofolate reductase Compounds References
Mycobacterium avium Dihydrofolate reductase Compounds References
Homo sapiens dihydrofolate reductase Compounds References
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcuslactis) Dihydrofolate reductase Compounds References
Bos taurus Dihydrofolate reductase Compounds References
Gallus gallus Dihydrofolate reductase Compounds References
Staphylococcus aureus Dihydrofolate reductase Compounds References
Lactobacillus casei Dihydrofolate reductase Compounds References
Staphylococcus aureus subsp. aureus MW2 Dihydrofolate reductase Compounds References
Bacillus anthracis Dihydrofolate reductase Compounds References
Neisseria gonorrhoeae Dihydrofolate reductase Compounds References
Mycobacterium leprae DIHYDROFOLATE REDUCTASE DFRA (DHFR) (TETRAHYDROFOLATE DEHYDROGENASE) Compounds References
Saccharomyces cerevisiae dihydrofolate reductase Compounds References
Salmonella enterica subsp. enterica serovar Typhi Dihydrofolate reductase Compounds References
Pneumocystis carinii Dihydrofolate reductase Compounds References
Enterococcus faecium Dihydrofolate reductase Compounds References
Mycobacterium tuberculosis Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase) Compounds References
Escherichia coli dihydrofolate reductase Compounds References
Mus musculus dihydrofolate reductase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Escherichia coli Dihydrofolate reductase type 1 157 aa 28.2% 131 aa Compounds References
Escherichia coli Dihydrofolate reductase 157 aa 29.5% 149 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Inhibition of dihydrofolate reductase in C. albicans (in vitro). ChEMBL
  • Inhibition of dihydrofolate reductase in Candida albicans (in vitro).
  • Ratio of I50 evaluated for inhibition of DHFR in humans to C. albicans ChEMBL
  • Ratio of I50 evaluated for inhibition of DHFR in humans to Candida albicans
  • Inhibitory activity of the compound against cell free dihydrofolate redutase (DHFR) from Escherichia coli ChEMBL
  • Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Escherichia coli
  • Inhibitory activity of the compound against cell free dihydrofolate redutase (DHFR) from Mycobacterium lufu ChEMBL
  • Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Mycobacterium lufu
  • Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Candida albicans ChEMBL
  • Inhibitory activity against cell free dihydrofolate reductase (DHFR) from Candida albicans
  • Inhibition of Dihydrofolate reductase enzyme from Candida albicans ChEMBL
  • Inhibition of Dihydrofolate reductase enzyme from Candida albicans
  • Inhibitory activity against C. albicans Dihydrofolate Reductase (DHFR) ChEMBL
  • Inhibitory activity against Candida albicans Dihydrofolate Reductase (DHFR)
  • ChEMBL
  • Inhibition of Candida albicans DHFR
  • ChEMBL
  • Inhibition of Candida albicans DHFR expressed in Escherichia coli BL21 (DE3) assessed as rate of NADPH consumption using dihydrofolate as substrate
  • ChEMBL
  • Binding affinity to Candida albicans DHFR at 1 mM incubated for 2 hrs by crystallographic analysis in presence of 1 mM NADPH
  • ChEMBL
  • Inhibition of Candida albicans DHFR
  • ChEMBL
  • Inhibition of Candida albicans DHFR using dihydrofolate as substrate preincubated for 5 mins followed by substrate addition in presence of NADPH
  • ChEMBL
  • Competitive inhibition of Candida albicans DHFR preincubated for 2 mins followed by substrate addition in presence of dihydrofolate

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier CaO19.5142 (Candida albicans), dihydrofolate reductase
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