Detailed view for Rv3116

Basic information

TDR Targets ID: 6515
Mycobacterium tuberculosis, Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase)
Source Database / ID:  Tuberculist 

pI: 5.084 | Length (AA): 389 | MW (Da): 41668 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00581   Rhodanese-like domain
PF00899   ThiF family

Gene Ontology

Mouse over links to read term descriptions.
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

Sulfur relay system (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 259 1jw9 (B) 4 248 41.00 0 1 1.19509 -1.02
14 259 1zfn (B) 1 244 38.00 0 1 1.10649 -0.83
17 323 1tt5 (B) 21 1007 29.00 0 1 0.751303 0.28
182 385 4f67 (A) 9 215 20.00 0 1 0.624522 0.6
286 380 3nhv (A) 33 128 27.00 0 1 0.652316 -1.12
303 378 1uar (A) 167 272 47.00 0.084 1 0.484073 -0.28
303 378 5ve3 (B) 270 345 45.00 0.000001 1 0.775073 -0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_126966)

Species Accession Gene Product
Arabidopsis thaliana AT5G55130   adenylyltransferase and sulfurtransferase MOCS3
Babesia bovis BBOV_IV003870   molybdenum cofactor synthesis protein 3 / molybdopterin synthase sulphurylase, putative
Brugia malayi Bm1_39490   MoeZ/MoeB domain containing protein
Candida albicans CaO19.9860   ubiquitin-activating enzyme
Candida albicans CaO19.2324   ubiquitin-activating enzyme
Caenorhabditis elegans CELE_F42G8.6   Protein MOC-3
Cryptosporidium hominis Chro.20124   ENSANGP00000008492
Cryptosporidium parvum cgd2_1120   putative molybdopterin synthase sulphurylase
Dictyostelium discoideum DDB_G0267980   molybdenum cofactor synthesis 3
Drosophila melanogaster Dmel_CG13090   CG13090 gene product from transcript CG13090-RC
Escherichia coli b0826   molybdopterin synthase sulfurylase
Escherichia coli b3992   adenylyltransferase, modifies ThiS C-terminus
Echinococcus granulosus EgrG_000253400   adenylyltransferase and sulfurtransferase
Entamoeba histolytica EHI_118040   molybdenum cofactor synthesis protein3, putative
Echinococcus multilocularis EmuJ_000253400   adenylyltransferase and sulfurtransferase
Giardia lamblia GL50803_12853   Molybdopterin biosynthesis MoeB protein
Homo sapiens 27304   molybdenum cofactor synthesis 3
Leishmania braziliensis LbrM.27.1800   molybdopterin synthase sulphurylase-like protein, putative
Leishmania donovani LdBPK_271570.1   molybdopterin synthase sulphurylase-like protein, putative
Leishmania infantum LinJ.27.1570   molybdopterin synthase sulphurylase-like protein, putative
Leishmania mexicana LmxM.27.1670   molybdopterin synthase sulphurylase-like protein, putative
Loa Loa (eye worm) LOAG_00263   hypothetical protein
Mycobacterium leprae ML0817   PROBABLE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOEB1 (MPT-SYNTHASE SULFURYLASE) (MOLYBDOPTERIN SYNTHASE SULPHURYLASE)
Mus musculus ENSMUSG00000074576   molybdenum cofactor synthesis 3
Mycobacterium tuberculosis Rv3116   Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase)
Mycobacterium tuberculosis Rv3206c   Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase)
Mycobacterium ulcerans MUL_2527   molybdopterin biosynthesis-like protein MoeZ
Neospora caninum NCLIV_049260   hypothetical protein
Oryza sativa 9268394   Os02g0804600
Oryza sativa 4329515   Os02g0525400
Plasmodium berghei PBANKA_1141200   ubiquitin-activating enzyme, putative
Plasmodium falciparum PF3D7_1365400   ubiquitin-activating enzyme
Plasmodium knowlesi PKNH_1105900   ubiquitin-activating enzyme, putative
Plasmodium vivax PVX_115230   ubiquitin-activating enzyme, putative
Plasmodium yoelii PY02846   molybdopterin biosynthesis protein MoeB
Saccharomyces cerevisiae YHR111W   Uba4p
Schistosoma japonicum Sjp_0041970   ko:K03751 molybdopterin biosynthesis protein MoeB, putative
Schistosoma mansoni Smp_035280   ubiquitin-activating enzyme E1
Schmidtea mediterranea mk4.004929.00   Adenylyltransferase and sulfurtransferase MOCS3
Schmidtea mediterranea mk4.085557.01  
Schmidtea mediterranea mk4.000957.11   Adenylyltransferase and sulfurtransferase MOCS3
Trypanosoma brucei gambiense Tbg972.11.2580   molybdopterin synthase sulphurylase protein, putative
Trypanosoma brucei Tb927.11.2330   molybdopterin synthase sulphurylase protein, putative
Trypanosoma congolense TcIL3000.11.2090   molybdopterin synthase sulphurylase protein, putative
Trypanosoma cruzi TcCLB.506127.80   molybdopterin synthase sulphurylase-like protein, putative
Toxoplasma gondii TGME49_212100   ThiF family protein
Theileria parva TP01_1017   UBA/THIF-type NAD/FAD binding protein, putative
Trichomonas vaginalis TVAG_318990   ubiquitin-activating enzyme E1c, putative
Trichomonas vaginalis TVAG_301440   ubiquitin-activating enzyme E1, putative

Essentiality

Rv3116 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3169 this record Mycobacterium tuberculosis non-essential nmpdr
Tb11.22.0008 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.22.0008 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.22.0008 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.22.0008 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0826 Escherichia coli non-essential goodall
b3992 Escherichia coli non-essential goodall
PBANKA_1141200 Plasmodium berghei Essential plasmo
TGME49_212100 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0005 0.3625 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Rv3116 (Mycobacterium tuberculosis), Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase)
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