Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | endoplasmic reticulum to nucleus signaling 1 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | serine:threonine protein kinase:endoribonuclease | 0.0121 | 0.0256 | 0.0235 |
Loa Loa (eye worm) | IRE protein kinase | 0.0121 | 0.0256 | 1 |
Echinococcus multilocularis | serine:threonine protein kinase:endoribonuclease | 0.0121 | 0.0256 | 0.0235 |
Echinococcus multilocularis | serine:threonine protein kinase | 0.2422 | 1 | 1 |
Loa Loa (eye worm) | CAMK protein kinase | 0.0068 | 0.0032 | 0.0481 |
Echinococcus granulosus | titin | 0.0068 | 0.0032 | 0.0011 |
Entamoeba histolytica | protein kinase, putative | 0.0121 | 0.0256 | 0.5 |
Schistosoma mansoni | cell adhesion molecule | 0.0065 | 0.0021 | 0.4637 |
Onchocerca volvulus | 0.0068 | 0.0032 | 1 | |
Entamoeba histolytica | protein kinase, putative | 0.0121 | 0.0256 | 0.5 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0068 | 0.0032 | 1 |
Loa Loa (eye worm) | CAMK/MLCK protein kinase | 0.0068 | 0.0032 | 0.0481 |
Schistosoma mansoni | cell adhesion molecule | 0.0065 | 0.0021 | 0.4637 |
Schistosoma mansoni | nephrin | 0.0065 | 0.0021 | 0.4637 |
Echinococcus granulosus | serine:threonine protein kinase | 0.2422 | 1 | 1 |
Schistosoma mansoni | titin | 0.0065 | 0.0021 | 0.4637 |
Schistosoma mansoni | neuroglian | 0.0065 | 0.0021 | 0.4637 |
Schistosoma mansoni | nephrin | 0.0065 | 0.0021 | 0.4637 |
Echinococcus multilocularis | myosin light chain kinase, smooth muscle | 0.2422 | 1 | 1 |
Schistosoma mansoni | hemicentin | 0.0065 | 0.0021 | 0.4637 |
Schistosoma mansoni | titin | 0.0068 | 0.0032 | 1 |
Schistosoma mansoni | cell adhesion molecule | 0.0065 | 0.0021 | 0.4637 |
Schistosoma mansoni | hypothetical protein | 0.0065 | 0.0021 | 0.4637 |
Echinococcus granulosus | titin | 0.0068 | 0.0032 | 0.0011 |
Brugia malayi | Immunoglobulin I-set domain containing protein | 0.0068 | 0.0032 | 0.0481 |
Schistosoma mansoni | receptor tyrosine phosphatase type r2a | 0.0065 | 0.0021 | 0.4637 |
Brugia malayi | Ribonuclease 2-5A family protein | 0.0121 | 0.0256 | 1 |
Schistosoma mansoni | myosin-binding protein-related | 0.0065 | 0.0021 | 0.4637 |
Brugia malayi | protein unc-22 | 0.0068 | 0.0032 | 0.0481 |
Echinococcus multilocularis | titin | 0.0068 | 0.0032 | 0.0011 |
Schistosoma mansoni | cell adhesion molecule | 0.0065 | 0.0021 | 0.4637 |
Loa Loa (eye worm) | CAMK/MLCK protein kinase | 0.0068 | 0.0032 | 0.0481 |
Trichomonas vaginalis | serine threonine-protein kinase, putative | 0.006 | 0 | 0.5 |
Trichomonas vaginalis | conserved hypothetical protein | 0.006 | 0 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | < 100 nM | BindingDB_Patents: Inhibition Assay. A fusion protein comprising glutathione S transferase (GST) and human IRE-1alpha (GST-IRE-1alpha) obtained from a 500 ml baculovirus-infected insect cell culture can be used to measure IRE-1alpha activity in vitro. Five ul of a reaction mixture comprising IX reaction buffer (5x reaction buffer is 100 mM Hepes pH 7.5, 250 mM KOAc, 2.5 mM MgCl2), 3 mM DTT, and 0.4% polyethylene glycol water is added to each well of 384 well plates. Twenty-five nanoliters of a 1 mM test compound solution are added to test wells. Three ul of a 128 ng/ml IRE-1alpha preparation are added to each test well and to positive control wells (final concentration 5.82 ng/well). Negative control wells contain only reaction mixture and test compound.After spinning the plates at 1200 rpm for 30 seconds, 3 ul of an IRE-1alpha human mini-XBP-1 mRNA stem-loop substrate 5'-CAGUCCGCAGCACUG-3' (SEQ ID NO:1), labeled with the fluorescent dye Cy5 at the 5' end and Black Hole Quencher 2. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.