Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | transmembrane protease, serine 15 | Starlite/ChEMBL | No references |
Homo sapiens | protease, serine, 1 (trypsin 1) | Starlite/ChEMBL | No references |
Species | Potential target | Known druggable target | Length | Alignment span | Identity |
---|---|---|---|---|---|
Brugia malayi | Trypsin family protein | protease, serine, 1 (trypsin 1) | 247 aa | 287 aa | 21.6 % |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Loa Loa (eye worm) | dUTP diphosphatase | 0.0331 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0116 | 0.3126 | 0.3126 |
Onchocerca volvulus | 0.0019 | 0.0044 | 0.014 | |
Mycobacterium ulcerans | deoxyuridine 5'-triphosphate nucleotidohydrolase | 0.0115 | 0.3095 | 0.5 |
Treponema pallidum | deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) | 0.0331 | 1 | 0.5 |
Onchocerca volvulus | 0.0019 | 0.0044 | 0.014 | |
Brugia malayi | Protein kinase domain containing protein | 0.0019 | 0.0044 | 0.0044 |
Mycobacterium tuberculosis | Probable deoxycytidine triphosphate deaminase Dcd (dCTP deaminase) | 0.0115 | 0.3095 | 0.5 |
Schistosoma mansoni | hypothetical protein | 0.0019 | 0.0044 | 0.0044 |
Schistosoma mansoni | subfamily S1A unassigned peptidase (S01 family) | 0.0123 | 0.3339 | 0.3339 |
Plasmodium falciparum | deoxyuridine 5'-triphosphate nucleotidohydrolase | 0.0331 | 1 | 0.5 |
Entamoeba histolytica | hypothetical protein | 0.0331 | 1 | 1 |
Mycobacterium leprae | PROBABLE DEOXYCYTIDINE TRIPHOSPHATE DEAMINASE DCD (DCTP DEAMINASE) | 0.0115 | 0.3095 | 0.5 |
Trichomonas vaginalis | deoxyuridine 5'-triphosphate nucleotidohydrolase, putative | 0.0331 | 1 | 0.5 |
Echinococcus granulosus | dUTP pyrophosphatase | 0.0331 | 1 | 1 |
Plasmodium vivax | deoxyuridine 5'-triphosphate nucleotidohydrolase, putative | 0.0331 | 1 | 0.5 |
Entamoeba histolytica | hypothetical protein | 0.0216 | 0.6328 | 0.4682 |
Loa Loa (eye worm) | hypothetical protein | 0.0019 | 0.0044 | 0.0044 |
Brugia malayi | Trypsin family protein | 0.0116 | 0.3126 | 0.3126 |
Schistosoma mansoni | deoxyuridine 5'-triphosphate nucleotidohydrolase | 0.0331 | 1 | 1 |
Entamoeba histolytica | hypothetical protein | 0.0216 | 0.6328 | 0.4682 |
Entamoeba histolytica | deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial precursor, putative | 0.0331 | 1 | 1 |
Entamoeba histolytica | deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial precursor, putative | 0.0331 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0019 | 0.0044 | 0.0044 |
Entamoeba histolytica | deoxyuridine 5-triphosphate nucleotidohydrolase domain containing protein | 0.0331 | 1 | 1 |
Loa Loa (eye worm) | TK/ALK protein kinase | 0.0019 | 0.0044 | 0.0044 |
Loa Loa (eye worm) | hypothetical protein | 0.0116 | 0.3126 | 0.3126 |
Entamoeba histolytica | hypothetical protein | 0.0331 | 1 | 1 |
Schistosoma mansoni | hypothetical protein | 0.0019 | 0.0044 | 0.0044 |
Onchocerca volvulus | 0.0106 | 0.2821 | 0.9025 | |
Entamoeba histolytica | deoxyuridine 5-triphosphate nucleotidohydrolase domain containing protein | 0.0331 | 1 | 1 |
Onchocerca volvulus | 0.0116 | 0.3126 | 1 | |
Mycobacterium ulcerans | deoxycytidine triphosphate deaminase | 0.0115 | 0.3095 | 0.5 |
Schistosoma mansoni | subfamily S1A unassigned peptidase (S01 family) | 0.0116 | 0.3126 | 0.3126 |
Toxoplasma gondii | deoxyuridine 5'-triphosphate nucleotidohydrolase, putative | 0.0331 | 1 | 0.5 |
Chlamydia trachomatis | deoxyuridine 5'-triphosphate nucleotidohydrolase | 0.0331 | 1 | 1 |
Entamoeba histolytica | deoxyuridine 5-triphosphate nucleotidohydrolase domain containing protein | 0.0331 | 1 | 1 |
Echinococcus multilocularis | dUTP pyrophosphatase | 0.0331 | 1 | 1 |
Brugia malayi | hypothetical protein | 0.0019 | 0.0044 | 0.0044 |
Wolbachia endosymbiont of Brugia malayi | dUTPase | 0.0331 | 1 | 1 |
Entamoeba histolytica | deoxyuridine 5-triphosphate nucleotidohydrolase domain containing protein | 0.0331 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0019 | 0.0044 | 0.0044 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
Ki (binding) | = 1.53 nM | BindingDB_Patents: Inhibition Assay. Using a 96 well plate (#3915, Costar), a test compound (25 µL) was mixed with 20 µM fluorescence enzyme substrate (Boc-Phe-Ser-Arg-AMC, 50 µL) mixed with 200 mM Tris-HCl buffer (pH 8.0), and human trypsin (Sigma, 25 µL) was added. Using a fluorescence plate reader fmax (Molecular Devices, Inc.), the reaction rate was measured from the time-course changes at excitation wavelength 355 nm and fluorescence wavelength 460 nm. | ChEMBL. | No reference |
Ki (binding) | = 1.76 nM | BindingDB_Patents: Inhibition Assay. Using a 96 well plate (#3915, Costar), a test compound (25 µL), 400 mM Tris-HCl buffer (pH 8.0, 25 µL) and 0.5 mg/mL fluorescence enzyme substrate (Gly-Asp-Asp-Asp-Asp-Lys-ß-Naphtylamide, 25 µL) were mixed, and recombinant human enteropeptidase (R&D Systems, Inc., 25 µL) was added. Using a fluorescence plate reader fmax (Molecular Devices, Inc.), the reaction rate was measured from the time-course changes at excitation wavelength 320 nm and fluorescence wavelength 405 nm. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.