Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | B-Raf proto-oncogene, serine/threonine kinase | Starlite/ChEMBL | No references |
Species | Potential target | Known druggable target/s | Ortholog Group |
---|---|---|---|
Echinococcus multilocularis | raf serine:threonine protein kinase | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Brugia malayi | Raf kinase | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Echinococcus granulosus | raf serine:threonine protein kinase | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Schistosoma japonicum | ko:K04365 B-Raf proto-oncogene serine/threonine-protein kinase, putative | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Loa Loa (eye worm) | raf kinase | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Schistosoma mansoni | serine/threonine protein kinase | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Loa Loa (eye worm) | TKL/RAF/RAF protein kinase | Get druggable targets OG5_130459 | All targets in OG5_130459 |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Schistosoma mansoni | retinoic acid receptor RXR | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor family member nhr-41 | 0.0149 | 0.3851 | 0.4053 |
Brugia malayi | nuclear hormone receptor | 0.0149 | 0.3851 | 0.4053 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Schistosoma mansoni | RAR-like nuclear receptor | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | TKL/RAF/RAF protein kinase | 0.0148 | 0.3802 | 0.3829 |
Echinococcus granulosus | FTZ F1 nuclear receptor protein | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor family member nhr-25 | 0.0149 | 0.3851 | 0.4053 |
Onchocerca volvulus | Protein ultraspiracle homolog | 0.0149 | 0.3851 | 0.5 |
Echinococcus multilocularis | raf serine:threonine protein kinase | 0.0262 | 1 | 1 |
Brugia malayi | Ligand-binding domain of nuclear hormone receptor family protein | 0.0149 | 0.3851 | 0.4053 |
Loa Loa (eye worm) | nuclear hormone receptor family member nhr-14 | 0.0149 | 0.3851 | 0.3878 |
Schistosoma mansoni | coup transcription factor | 0.0149 | 0.3851 | 0.3851 |
Echinococcus multilocularis | hepatocyte nuclear factor 4 alpha | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Schistosoma mansoni | thyroid hormone receptor | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | photoreceptor-specific nuclear receptor | 0.0149 | 0.3851 | 0.4053 |
Schistosoma mansoni | FTZ-F1 nuclear receptor-like protein | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor-like 1 | 0.0149 | 0.3851 | 0.4053 |
Loa Loa (eye worm) | nuclear hormone receptor family member nhr-41 | 0.0149 | 0.3851 | 0.3878 |
Echinococcus multilocularis | nuclear receptor 2DBD gamma | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Ligand-binding domain of nuclear hormone receptor family protein | 0.0149 | 0.3851 | 0.4053 |
Schistosoma mansoni | steroid hormone receptor ad4bp | 0.0149 | 0.3851 | 0.3851 |
Echinococcus granulosus | nuclear receptor 2DBD gamma | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor family member nhr-25 | 0.0149 | 0.3851 | 0.4053 |
Brugia malayi | Nuclear hormone receptor family member nhr-19 | 0.0149 | 0.3851 | 0.4053 |
Loa Loa (eye worm) | nuclear hormone receptor family member nhr-1 | 0.0149 | 0.3851 | 0.3878 |
Brugia malayi | nuclear receptor NHR-88 | 0.0149 | 0.3851 | 0.4053 |
Schistosoma mansoni | photoreceptor-specific nuclear receptor related | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor family member nhr-31 | 0.0149 | 0.3851 | 0.4053 |
Echinococcus multilocularis | Nuclear hormone receptor family member nhr 41 | 0.0149 | 0.3851 | 0.3851 |
Onchocerca volvulus | Steroid hormone receptor family member cnr14 homolog | 0.0149 | 0.3851 | 0.5 |
Loa Loa (eye worm) | raf kinase | 0.026 | 0.993 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Echinococcus granulosus | ecdysone induced protein 78C | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | nuclear Hormone Receptor family member | 0.0149 | 0.3851 | 0.3878 |
Brugia malayi | Nuclear hormone receptor family member nhr-49 | 0.0149 | 0.3851 | 0.4053 |
Schistosoma mansoni | retinoid-x-receptor (RXR) | 0.0149 | 0.3851 | 0.3851 |
Echinococcus granulosus | nuclear receptor 2DBD gamma | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | nuclear hormone receptor family member nhr-31 | 0.0149 | 0.3851 | 0.3878 |
Schistosoma mansoni | thyroid hormone receptor | 0.0149 | 0.3851 | 0.3851 |
Schistosoma mansoni | nuclear hormone receptor | 0.0149 | 0.3851 | 0.3851 |
Onchocerca volvulus | Bile acid receptor homolog | 0.0149 | 0.3851 | 0.5 |
Echinococcus multilocularis | ecdysone induced protein 78C | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Steroid receptor seven-up type 2 | 0.0149 | 0.3851 | 0.4053 |
Onchocerca volvulus | 0.0149 | 0.3851 | 0.5 | |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Brugia malayi | Nuclear hormone receptor family member nhr-40 | 0.0149 | 0.3851 | 0.4053 |
Echinococcus multilocularis | FTZ F1 nuclear receptor protein | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Echinococcus granulosus | Nuclear hormone receptor family member nhr 41 | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Schistosoma mansoni | Tr4/Tr2 (homologue) | 0.0149 | 0.3851 | 0.3851 |
Schistosoma mansoni | nuclear receptor 2DBD-gamma | 0.0149 | 0.3851 | 0.3851 |
Echinococcus multilocularis | COUP TF:Svp nuclear hormone receptor | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | nuclear hormone receptor family member nhr-49 | 0.0149 | 0.3851 | 0.3878 |
Echinococcus granulosus | COUP TF:Svp nuclear hormone receptor | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | steroid hormone receptor | 0.0149 | 0.3851 | 0.4053 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Brugia malayi | Nuclear hormone receptor family member nhr-14 | 0.0149 | 0.3851 | 0.4053 |
Echinococcus granulosus | FTZ F1 alpha | 0.0149 | 0.3851 | 0.3851 |
Echinococcus granulosus | hepatocyte nuclear factor 4 alpha | 0.0149 | 0.3851 | 0.3851 |
Loa Loa (eye worm) | steroid hormone receptor | 0.0149 | 0.3851 | 0.3878 |
Echinococcus multilocularis | nuclear receptor 2DBD gamma | 0.0149 | 0.3851 | 0.3851 |
Echinococcus multilocularis | thyroid hormone receptor alpha | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | ecdysteroid receptor | 0.0149 | 0.3851 | 0.4053 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0262 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0149 | 0.3851 | 0.3878 |
Loa Loa (eye worm) | nuclear hormone receptor family member nhr-40 | 0.0149 | 0.3851 | 0.3878 |
Brugia malayi | Raf kinase | 0.0253 | 0.9501 | 1 |
Echinococcus multilocularis | FTZ F1 alpha | 0.0149 | 0.3851 | 0.3851 |
Schistosoma mansoni | nuclear hormone receptor nor-1/nor-2 | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor family member nhr-3 | 0.0149 | 0.3851 | 0.4053 |
Brugia malayi | Nuclear hormone receptor family member nhr-19 | 0.0149 | 0.3851 | 0.4053 |
Echinococcus granulosus | retinoic acid receptor rxr beta a | 0.0149 | 0.3851 | 0.3851 |
Brugia malayi | Nuclear hormone receptor family member nhr-1 | 0.0149 | 0.3851 | 0.4053 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 5 nM | BindingDB_Patents: kinase Assay. The final concentration of DMSO is 5%. 10 µL of the B-Raf (V600E)-kinase solution are pipetted in (containing 0.5 ng B-Raf (V600E)-kinase in 20 mM Tris-HCl pH 7.5, 0.1 mM EDTA, 0.1 mM EGTA, 0.286 mM sodium orthovanadate, 10% glycerol, 1 mg/mL bovine serum albumin, 1 mM dithiothreitol) and the mixture is incubated for 24 h at RT under with shaking. The kinase reaction is started by the addition of 20 µL ATP solution [final concentration: 250 µM ATP, 30 mM Tris-HCl pH 7.5, 0.02% Brij, 0.2 mM sodium orthovanadate, 10 mM magnesium acetate, 0.1 mM EGTA, phosphatase cocktail (Sigma, # P2850, dilution recommended by the manufacturer), 0.1 mM EGTA] and 10 µL MEK1 solution [containing 50 ng biotinylated MEK1 (prepared from purified MEK1 according to standard procedure, e.g. with EZ-Link Sulpho-NHS-LC-Biotin reagent, Pierce, #21335) and carried out for 60 min at RT with constant shaking. | ChEMBL. | No reference |
IC50 (binding) | = 5 nM | kinase Assay | BINDINGDB. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.