Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | lipase, hepatic | Starlite/ChEMBL | No references |
Homo sapiens | lipase, endothelial | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Onchocerca volvulus | 0.0048 | 0.183 | 0.4455 | |
Brugia malayi | hypothetical protein | 0.0048 | 0.183 | 0.4811 |
Echinococcus granulosus | conserved hypothetical protein | 0.0013 | 0.0244 | 0.0196 |
Echinococcus multilocularis | Polycystic kidney disease protein | 0.0048 | 0.183 | 0.1892 |
Mycobacterium ulcerans | transmembrane ABC transporter ATP-binding protein | 0.0009 | 0.0062 | 0.5 |
Trypanosoma brucei | ABC transporter, putative | 0.0009 | 0.0062 | 0.5 |
Echinococcus multilocularis | lipoxygenase domain containing protein | 0.0048 | 0.183 | 0.1892 |
Echinococcus multilocularis | conserved hypothetical protein | 0.0013 | 0.0244 | 0.0196 |
Echinococcus granulosus | Polycystic kidney disease protein | 0.0048 | 0.183 | 0.1892 |
Plasmodium vivax | hypothetical protein, conserved | 0.0013 | 0.0244 | 0.1034 |
Plasmodium falciparum | LCCL domain-containing protein | 0.0048 | 0.183 | 1 |
Brugia malayi | Uncharacterized hematopoietic stem/progenitor cells protein MDS029 | 0.0091 | 0.3804 | 1 |
Echinococcus granulosus | RUN | 0.0048 | 0.183 | 0.1892 |
Plasmodium falciparum | CDGSH iron-sulfur domain-containing protein, putative | 0.0013 | 0.0244 | 0.1034 |
Onchocerca volvulus | 0.0048 | 0.183 | 0.4455 | |
Echinococcus multilocularis | cdgsh iron sulfur domain containing protein | 0.0214 | 0.9412 | 1 |
Schistosoma mansoni | rab6-interacting | 0.0048 | 0.183 | 0.183 |
Brugia malayi | hypothetical protein | 0.0048 | 0.183 | 0.4811 |
Loa Loa (eye worm) | hypothetical protein | 0.0048 | 0.183 | 0.4811 |
Schistosoma mansoni | rab6-interacting | 0.0048 | 0.183 | 0.183 |
Schistosoma mansoni | loxhd1 | 0.0048 | 0.183 | 0.183 |
Echinococcus granulosus | cdgsh iron sulfur domain containing protein | 0.0214 | 0.9412 | 1 |
Trypanosoma brucei | ATP-binding cassette protein, putative | 0.0009 | 0.0062 | 0.5 |
Onchocerca volvulus | 0.0091 | 0.3804 | 1 | |
Plasmodium falciparum | CDGSH iron-sulfur domain-containing protein, putative | 0.0013 | 0.0244 | 0.1034 |
Loa Loa (eye worm) | hypothetical protein | 0.0013 | 0.0244 | 0.0643 |
Schistosoma mansoni | hypothetical protein | 0.0013 | 0.0244 | 0.0244 |
Echinococcus granulosus | lipoxygenase domain containing protein | 0.0048 | 0.183 | 0.1892 |
Entamoeba histolytica | ABC transporter, putative | 0.0009 | 0.0062 | 0.5 |
Toxoplasma gondii | zinc finger cdgsh type protein | 0.0013 | 0.0244 | 1 |
Schistosoma mansoni | hypothetical protein | 0.0048 | 0.183 | 0.183 |
Brugia malayi | RE40412p | 0.0013 | 0.0244 | 0.0643 |
Brugia malayi | RE40412p | 0.0013 | 0.0244 | 0.0643 |
Leishmania major | hypothetical protein, unknown function | 0.0013 | 0.0244 | 1 |
Brugia malayi | Doublecortin family protein | 0.0048 | 0.183 | 0.4811 |
Schistosoma mansoni | polycystin 1-related | 0.0048 | 0.183 | 0.183 |
Plasmodium vivax | hypothetical protein, conserved | 0.0013 | 0.0244 | 0.1034 |
Echinococcus multilocularis | RUN | 0.0048 | 0.183 | 0.1892 |
Plasmodium vivax | multidomain scavenger receptor, putative | 0.0048 | 0.183 | 1 |
Toxoplasma gondii | PfMNL-2 CISD1 family iron-sulfur protein, putative | 0.0013 | 0.0244 | 1 |
Mycobacterium ulcerans | transmembrane ABC transporter ATP-binding protein | 0.0009 | 0.0062 | 0.5 |
Toxoplasma gondii | zinc finger CDGSH-type domain-containing protein | 0.0013 | 0.0244 | 1 |
Plasmodium falciparum | CDGSH iron-sulfur domain-containing protein, putative | 0.0013 | 0.0244 | 0.1034 |
Schistosoma mansoni | hypothetical protein | 0.0227 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0091 | 0.3804 | 1 |
Mycobacterium ulcerans | transmembrane ABC transporter ATP-binding protein | 0.0009 | 0.0062 | 0.5 |
Trypanosoma cruzi | ABC transporter, putative | 0.0009 | 0.0062 | 0.5 |
Loa Loa (eye worm) | doublecortin family protein | 0.0048 | 0.183 | 0.4811 |
Plasmodium vivax | hypothetical protein, conserved | 0.0013 | 0.0244 | 0.1034 |
Echinococcus multilocularis | lipoxygenase domain containing protein | 0.0048 | 0.183 | 0.1892 |
Trypanosoma cruzi | ATP-binding cassette protein, putative | 0.0009 | 0.0062 | 0.5 |
Echinococcus granulosus | lipoxygenase domain containing protein | 0.0048 | 0.183 | 0.1892 |
Loa Loa (eye worm) | hypothetical protein | 0.0048 | 0.183 | 0.4811 |
Loa Loa (eye worm) | hypothetical protein | 0.0013 | 0.0244 | 0.0643 |
Schistosoma mansoni | ATP-binding cassette sub-family g2 (white protein) (abcg2) | 0.0009 | 0.0062 | 0.0062 |
Schistosoma mansoni | lipoxygenase | 0.0048 | 0.183 | 0.183 |
Giardia lamblia | ABC transporter | 0.0009 | 0.0057 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 1.8 nM | Biological Assay | BINDINGDB. | No reference |
IC50 (binding) | = 4 nM | Biological Assay | BINDINGDB. | No reference |
IC50 (binding) | = 261.6 nM | BindingDB_Patents: Biological Assay. Endothelial lipase (EL) and hepatic lipase (HL) activities were measured using a fluorescent substrate, A10070, (Invitrogen, CA) doped into an artificial vesicle containing DMPG (Avanti Polar Lipids) as the excipient. Vesicles were prepared by combining 571 uL of 29 mM DMPG in a 1:1 mixture of MeOH and CHCl3 with 2000 uL of 1 mM A10070 in a 1:1 mixture of MeOH and CHCl3. The mixture was dried under nitrogen in multiple vials then resuspended in 20 mL total volume of 50 mM HEPES pH 8.0 buffer containing 50 mM NaCl and 0.2 mM EDTA. The sample was allowed to sit at room temperature for 15 min and then was sonicated 3x4 mins on ice with a Branson Sonicator using duty cycle 1. This preparation provides vesicles with a mole fraction of 0.11 for the FRET substrate.The enzymatic assay was measured using 384-well white Optiplates. Each well contained 20 uL of assay buffer (50 mM HEPES pH 8.0, 50 mM NaCl and 1 mM CaCl2) and 0.25 uL of a DMSO solution containing a compound. | ChEMBL. | No reference |
IC50 (binding) | = 2624 nM | BindingDB_Patents: Biological Assay. Endothelial lipase (EL) and hepatic lipase (HL) activities were measured using a fluorescent substrate, A10070, (Invitrogen, CA) doped into an artificial vesicle containing DMPG (Avanti Polar Lipids) as the excipient. Vesicles were prepared by combining 571 uL of 29 mM DMPG in a 1:1 mixture of MeOH and CHCl3 with 2000 uL of 1 mM A10070 in a 1:1 mixture of MeOH and CHCl3. The mixture was dried under nitrogen in multiple vials then resuspended in 20 mL total volume of 50 mM HEPES pH 8.0 buffer containing 50 mM NaCl and 0.2 mM EDTA. The sample was allowed to sit at room temperature for 15 min and then was sonicated 3x4 mins on ice with a Branson Sonicator using duty cycle 1. This preparation provides vesicles with a mole fraction of 0.11 for the FRET substrate.The enzymatic assay was measured using 384-well white Optiplates. Each well contained 20 uL of assay buffer (50 mM HEPES pH 8.0, 50 mM NaCl and 1 mM CaCl2) and 0.25 uL of a DMSO solution containing a compound. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.