Detailed view for Tb927.8.6390

Basic information

TDR Targets ID: 19233
Trypanosoma brucei, lysophospholipase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4924 | Length (AA): 280 | MW (Da): 30092 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02230   Phospholipase/Carboxylesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016788   hydrolase activity, acting on ester bonds  
GO:0005575   cellular_component  
GO:0016787   hydrolase activity  
GO:0008152   metabolic process  

Metabolic Pathways

Glycerophospholipid metabolism (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127329)

Species Accession Gene Product
Arabidopsis thaliana AT4G22300   carboxylesterase
Arabidopsis thaliana AT4G22305   carboxylesterase
Arabidopsis thaliana AT5G20060   phospholipase/carboxylesterase family protein
Brugia malayi Bm1_22915   Phospholipase/Carboxylesterase family protein
Candida albicans CaO19.4248   likely Phospholipase/Carboxylesterase similar to S. cerevisiae YLR118C
Candida albicans CaO19.11723   likely Phospholipase/Carboxylesterase similar to S. cerevisiae YLR118C
Caenorhabditis elegans CELE_K04G2.5   Protein ATH-1
Cryptosporidium hominis Chro.10371   carboxylesterase
Cryptosporidium hominis Chro.10422   hypothetical protein
Cryptosporidium parvum cgd1_3750   conserved hypothetical protein
Cryptosporidium parvum cgd1_3290   carboxylesterase, putative
Chlamydia trachomatis CT_136   lysophospholipase esterase
Dictyostelium discoideum DDB_G0268064   esterase/lipase/thioesterase domain-containing protein
Dictyostelium discoideum DDB_G0282005   phospholipase/carboxylesterase family protein
Drosophila melanogaster Dmel_CG18815   CG18815 gene product from transcript CG18815-RD
Echinococcus granulosus EgrG_000053900   acyl protein thioesterase 12
Echinococcus granulosus EgrG_000465300   acyl protein thioesterase 1
Echinococcus multilocularis EmuJ_000053900   acyl protein thioesterase 12
Echinococcus multilocularis EmuJ_000465300   acyl protein thioesterase 1
Homo sapiens ENSG00000011009   lysophospholipase II
Leishmania braziliensis LbrM.24.1910   lysophospholipase, putative
Leishmania donovani LdBPK_241910.1   lysophospholipase, putative
Leishmania infantum LinJ.24.1910   lysophospholipase, putative
Leishmania major LmjF.24.1840   lysophospholipase, putative
Leishmania mexicana LmxM.24.1840   lysophospholipase, putative
Loa Loa (eye worm) LOAG_03421   phospholipase/Carboxylesterase
Mus musculus ENSMUSG00000028670   lysophospholipase 2
Mus musculus ENSMUSG00000025903   lysophospholipase 1
Neospora caninum NCLIV_045170   hypothetical protein
Oryza sativa 4337344   Os04g0669500
Oryza sativa 4327131   Os01g0175000
Oryza sativa 4337346   Os04g0669700
Saccharomyces cerevisiae YLR118C   palmitoyl-(protein) hydrolase
Schistosoma japonicum Sjp_0304910   ko:K06130 lysophospholipase II, putative
Schistosoma mansoni Smp_025160.2   acyl-protein thioesterase 12 (lysophospholipase III)
Schistosoma mansoni Smp_025160.3   acyl-protein thioesterase 12 (lysophospholipase III)
Schistosoma mansoni Smp_025160.1   acyl-protein thioesterase 12 (lysophospholipase III)
Schmidtea mediterranea mk4.000432.04   Putative acyl-protein thioesterase 1,2
Trypanosoma brucei gambiense Tbg972.8.6450   lysophospholipase, putative,alpha/beta hydrolase, putative
Trypanosoma brucei Tb927.8.6390   lysophospholipase, putative
Trypanosoma congolense TcIL3000_8_6240   lysophospholipase, putative
Trypanosoma cruzi TcCLB.511907.50   lysophospholipase, putative
Trypanosoma cruzi TcCLB.506797.70   lysophospholipase, putative
Toxoplasma gondii TGME49_228290   phospholipase/carboxylesterase
Wolbachia endosymbiont of Brugia malayi Wbm0546   esterase

Essentiality

Tb927.8.6390 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6390 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.6390 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.6390 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6390 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_K04G2.5 Caenorhabditis elegans embryonic lethal wormbase
TGME49_228290 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens lysophospholipase II Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0062 0.5 0.5
0.0072 0.5 0.5
0.0073 0.5 0.5
0.0100633 0.5 0.5
0.0073 0.5 0.5
0.0067 0.5 0.5
0.0068 0.5 0.5
0.0073 0.5 0.5
0.0073 0.5 0.5
0.0073 0.5 0.5
0.0073 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru017501AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru017501; Recombinant protein: full-length; Source: T brucei; alpha/beta hydrolase, putative ;

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.8.6390 (Trypanosoma brucei), lysophospholipase, putative
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