Detailed view for PF3D7_1419300

Basic information

TDR Targets ID: 2519
Plasmodium falciparum, glutathione S-transferase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.2554 | Length (AA): 211 | MW (Da): 24789 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02798   Glutathione S-transferase, N-terminal domain
PF14497   Glutathione S-transferase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005515   protein binding  
GO:0004364   glutathione transferase activity  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 211 1okt (A) 1 211 99.99 0 1 2.32 -2.52
3 207 4zxg (A) 3 207 99.99 0 1 2.25566 -2.01

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1OKT:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1PA3:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1Q4J:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2AAW:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3FR3:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3FR6:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3FR9:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3FRC:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4ZXG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, gametocyte, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_128539)

Species Accession Gene Product
Caenorhabditis elegans CELE_T28A11.11   Protein GST-23
Caenorhabditis elegans CELE_F37B1.5   Protein GST-16
Dictyostelium discoideum DDB_G0280317   hypothetical protein
Dictyostelium discoideum DDB_G0283575   hypothetical protein
Dictyostelium discoideum DDB_G0274081   hypothetical protein
Dictyostelium discoideum DDB_G0268138   hypothetical protein
Dictyostelium discoideum DDB_G0272632   hypothetical protein
Echinococcus granulosus EgrG_000535300   glutathione s transferase mu
Echinococcus granulosus EgrG_000535200   glutathione S transferase
Echinococcus granulosus EgrG_000538900   glutathione S transferase
Echinococcus granulosus EgrG_000538700   glutathione S transferase
Echinococcus multilocularis EmuJ_000537600   glutathione S transferase
Echinococcus multilocularis EmuJ_000538300   glutathione S-transferase
Echinococcus multilocularis EmuJ_000535200   glutathione S transferase
Echinococcus multilocularis EmuJ_000535300   glutathione s transferase mu
Echinococcus multilocularis EmuJ_000538700   glutathione S transferase
Echinococcus multilocularis EmuJ_000538000   glutathione S transferase
Echinococcus multilocularis EmuJ_000538900   glutathione S transferase
Echinococcus multilocularis EmuJ_000005400   glutathione S transferase
Homo sapiens ENSG00000134202   glutathione S-transferase mu 3 (brain)
Homo sapiens ENSG00000213366   glutathione S-transferase mu 2 (muscle)
Homo sapiens ENSG00000168765   glutathione S-transferase mu 4
Mus musculus ENSMUSG00000027890   glutathione S-transferase, mu 4
Mus musculus ENSMUSG00000004035   glutathione S-transferase, mu 7
Mus musculus ENSMUSG00000004038   glutathione S-transferase, mu 3
Mus musculus ENSMUSG00000004032   glutathione S-transferase, mu 5
Plasmodium falciparum PF3D7_1419300   glutathione S-transferase
Schistosoma japonicum Sjp_0038300   ko:K00799 glutathione S-transferase [EC2.5.1.18], putative
Schistosoma mansoni Smp_102070   GST class-mu
Schistosoma mansoni Smp_163610   glutathione-S-transferase
Schmidtea mediterranea mk4.001107.01  
Schmidtea mediterranea mk4.000889.01  
Schmidtea mediterranea mk4.007945.00  

Essentiality

No essentiality data collected for this gene and/or its orthologs.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Plasmodium falciparum 87593   261984   536707  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 12108547

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

Compound Source Reference
ChEMBL23 References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens glutathione S-transferase mu 2 (muscle) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0154 0.3179 0.5
0.0238 0.348 1
0.0329 0.2873 1
0.0158 0.3179 0.5
0.0544 0.3725 1
0.0544 0.3729 1
0.0284 0.3524 1
0.0234 0.3275 1
0.0325 0.2856 1
0.0149 0.3179 0.5
0.0316 0.3179 0.5
0.0259 0.3525 1
0.0259 0.3525 1
0.0093 0.3179 0.5
0.0093 0.3179 0.5
0.0542 0.3791 1
0.0854 0.2742 0.5
0.0183 0.2771 1
0.0158 0.3491 1
0.0655 0.3179 0.5
0.0541 0.369 1
0.0408 0.3179 0.5
0.026 0.353 1
0.0386 0.3179 0.5
0.0093 0.3179 0.5
0.0068 0.2684 0.5
0.0281 0.3524 1
0.0752977 0.280277 0.823925
0.0542 0.3791 1
0.0721 0.3179 0.5
0.0298 0.2504 1
0.0093 0.3372 1
0.0328 0.2897 1
0.0541 0.369 1
0.0628 0.3179 0.5
0.0168 0.3179 0.5
0.0157 0.2684 0.5
0.0157 0.3179 0.5
0.0093 0.3179 0.5
0.0655 0.3179 0.5
0.0262 0.3525 1
0.0778 0.3179 0.5
0.0749206 0.356619 1
0.0286 0.3277 1
0.0623 0.3738 1
0.0553 0.2503 0.5
0.0542 0.3791 1
0.0314 0.3527 1
0.0701 0.2653 0.5
0.0259 0.3525 1
0.0229 0.3275 1
0.0093 0.2778 1
0.0393 0.3179 0.5
0.0168 0.3179 0.5
0.0162 0.2684 0.5
0.039 0.3179 0.5
0.0704 0.3 1
0.0055 0.3696 1
0.0889 0.3179 0.5
0.0542 0.3791 1
0.0555 0.3592 1
0.0395 0.3179 0.5
0.0093 0.3179 0.5
0.0239 0.3313 1
0.0086 0.3179 0.5
0.0239 0.3275 0.5575
0.0055 0.3696 1
0.0168 0.3179 0.5
0.0154 0.3179 0.5
0.0752977 0.280277 0.823925
0.0328 0.2897 1
0.0093 0.3179 0.5
0.1099 0.3179 0.5
0.0117 0.5128 1
0.0093 0.3179 0.5
0.0329 0.2759 1
0.0077 0.3179 0.5
0.0752977 0.280277 0.823925
0.0083 0.3179 0.5
0.0287 0.2573 1
0.0093 0.3311 0.5
0.0093 0.3179 0.5
0.0752977 0.280277 0.823925
0.0105289 0.295588 0.144861
0.0238 0.348 1
0.0243 0.3313 1
0.088 0.3179 0.5
0.0117 0.5128 1
0.0484 0.3179 0.5
0.0229 0.3179 0.5
0.0383 0.3179 0.5
0.1025 0.3786 1
0.0093 0.3034 0.5

Assayability

Assay information

  • Assay for GST 1-Chloro-2,4-Dinitrobenzene as Substrate (2.5.1.18 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • Effect of the compound on glutathione S-transferase (GST) activity of chloroquine resistant P. falciparum strain K1 in culture after 24 hr treatment at 150 nM conc ChEMBL
  • Effect on glutathione S-transferase (GST) activity of chloroquine-resistant Plasmodium falciparum K1 in culture after 24 hrs treatment at 150 nM conc
  • Effect of the compound on glutathione S-transferase (GST) activity of chloroquine resistant P. falciparum strain K1 in culture after 24 hr treatment at 30 nM conc ChEMBL
  • Effect on glutathione S-transferase (GST) activity of chloroquine-resistant Plasmodium falciparum K1 in culture after 24 hrs treatment at 30 nM conc
  • Effect of the compound on glutathione S-transferase (GST) activity of chloroquine resistant P. falciparum strain K1 in culture after 24 hr treatment at 500 nM conc ChEMBL
  • Effect on glutathione S-transferase (GST) activity of chloroquine-resistant Plasmodium falciparum K1 in culture after 24 hrs treatment at 500 nM conc
  • ChEMBL
  • Inhibition of Plasmodium falciparum glutathione S-transferase

Reagent availability

No reagent availability information for this target.

Bibliographic References

81 literature references were collected for this gene.

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Gene identifier PF3D7_1419300 (Plasmodium falciparum), glutathione S-transferase
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