Detailed view for PF3D7_0306300

Basic information

TDR Targets ID: 3564
Plasmodium falciparum, glutaredoxin 1
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.7445 | Length (AA): 111 | MW (Da): 12418 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00462   Glutaredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0015038   glutathione disulfide oxidoreductase activity  
GO:0015035   protein disulfide oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0045454   cell redox homeostasis  
GO:0006979   response to oxidative stress  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 109 1z7p (A) 8 109 27.00 0 1 1.48 -1.98
11 110 1kte () 4 104 38.00 0 1 1.58 -2.19
12 107 2fls (A) 20 113 31.00 0 1 1.47 -2.28
6 111 4mzc (A) 6 111 99.99 0 1 2.32746 -2.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 4HJM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4MZB:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4MZC:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4N0Z:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4N10:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4N11:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, gametocyte, sporozoite, early ring, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Oocyst, Ring, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Sporozoite. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_126864)

Species Accession Gene Product
Arabidopsis thaliana AT5G63030   glutaredoxin-C1
Arabidopsis thaliana AT5G20500   glutaredoxin-C4
Arabidopsis thaliana AT1G77370   glutaredoxin-C3
Arabidopsis thaliana AT5G40370   glutaredoxin-C2
Babesia bovis BBOV_IV004320   glutaredoxin-like protein, putative
Brugia malayi Bm1_48585   Glutaredoxin family protein
Brugia malayi Bm1_08085   Glutaredoxin family protein
Candida albicans CaO19.6510   potential glutaredoxin similar to S. cerevisiae GRX1 (YCL035C)
Candida albicans CaO19.6059   one of two potential glutaredoxin genes similar to S. cerevisiae TTR1 (YDR513W) mitochondrial glutaredoxin
Candida albicans CaO19.6509   potential glutaredoxin similar to plant glutaredoxins
Candida albicans CaO19.13863   potential glutaredoxin similar to S. cerevisiae GRX1 (YCL035C)
Candida albicans CaO19_6510   hypothetical protein
Candida albicans CaO19_6509   hypothetical protein
Candida albicans CaO19.13480   one of two potential glutaredoxin genes similar to S. cerevisiae TTR1 (YDR513W) mitochondrial glutaredoxin
Candida albicans CaO19.13862   potential glutaredoxin similar to plant glutaredoxins
Caenorhabditis elegans CELE_ZK121.1   Protein GLRX-21, isoform B
Caenorhabditis elegans CELE_Y34D9A.6   Protein GLRX-10
Cryptosporidium hominis Chro.20270   glutaredoxin
Cryptosporidium parvum cgd2_2540   glutaredoxin related protein
Dictyostelium discoideum DDB_G0290015   hypothetical protein
Dictyostelium discoideum DDB_G0271084   hypothetical protein
Drosophila melanogaster Dmel_CG7975   CG7975 gene product from transcript CG7975-RA
Drosophila melanogaster Dmel_CG6852   CG6852 gene product from transcript CG6852-RC
Escherichia coli b3610   glutaredoxin 3
Echinococcus granulosus EgrG_000124800   glutaredoxin 1
Echinococcus granulosus EgrG_000226900   glutaredoxin
Echinococcus multilocularis EmuJ_000124800   glutaredoxin 1
Echinococcus multilocularis EmuJ_000226900   glutaredoxin
Homo sapiens ENSG00000173221   glutaredoxin (thioltransferase)
Homo sapiens ENSG00000023572   glutaredoxin 2
Leishmania braziliensis LbrM.27.0881   glutaredoxin-like protein, putative
Leishmania donovani LdBPK_270670.1   glutaredoxin-like protein
Leishmania infantum LinJ.27.0670   glutaredoxin-like protein
Leishmania major LmjF.27.0810   glutaredoxin-like protein
Leishmania mexicana LmxM.27.0810   glutaredoxin-like protein
Loa Loa (eye worm) LOAG_05670   hypothetical protein
Loa Loa (eye worm) LOAG_11828   hypothetical protein
Loa Loa (eye worm) LOAG_13565   hypothetical protein
Mus musculus ENSMUSG00000018196   glutaredoxin 2 (thioltransferase)
Mus musculus ENSMUSG00000021591   glutaredoxin
Neospora caninum NCLIV_045900   hypothetical protein
Neospora caninum NCLIV_038390   hypothetical protein
Oryza sativa 4330145   Os02g0646400
Oryza sativa 4329999   Os02g0618100
Oryza sativa 4336354   Os04g0508300
Plasmodium berghei PBANKA_0404700   glutaredoxin 1, putative
Plasmodium falciparum PF3D7_0306300   glutaredoxin 1
Plasmodium knowlesi PKNH_0836400   glutaredoxin 1, putative
Plasmodium vivax PVX_119440   glutaredoxin 1, putative
Plasmodium yoelii PY05305   thioltransferase
Saccharomyces cerevisiae YCL035C   dithiol glutaredoxin GRX1
Saccharomyces cerevisiae YDR513W   dithiol glutaredoxin GRX2
Schmidtea mediterranea mk4.000383.06  
Schmidtea mediterranea mk4.000054.17  
Trypanosoma brucei gambiense Tbg972.1.990   glutaredoxin, putative
Trypanosoma brucei gambiense Tbg972.11.1450   glutaredoxin, putative
Trypanosoma brucei Tb927.1.1770   Dithiol glutaredoxin 2
Trypanosoma brucei Tb927.11.1370   Dithiol glutaredoxin 1
Trypanosoma cruzi TcCLB.506475.116   glutaredoxin, putative
Trypanosoma cruzi TcCLB.511431.40   glutaredoxin, putative
Toxoplasma gondii TGME49_227150   glutaredoxin, putative
Toxoplasma gondii TGME49_279400   glutaredoxin, putative
Theileria parva TP01_0320   glutaredoxin-like protein
Wolbachia endosymbiont of Brugia malayi Wbm0415   glutaredoxin

Essentiality

PF3D7_0306300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.47.0012 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.47.0012 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.47.0012 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.47.0012 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3610 Escherichia coli non-essential goodall
PBANKA_0404700 Plasmodium berghei Dispensable plasmo
TGME49_227150 Toxoplasma gondii Probably non-essential sidik
TGME49_279400 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_0306300 (Plasmodium falciparum), glutaredoxin 1
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