Detailed view for Rv0512

Basic information

TDR Targets ID: 980840
Mycobacterium tuberculosis, Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH)
Source Database / ID:  Tuberculist 

pI: 4.5194 | Length (AA): 329 | MW (Da): 34871 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Porphyrin and chlorophyll metabolism (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 329 1aw5 (A) 1 339 36.00 0 1 1.3516 0.02
1 328 5lzl (A) 1 332 50.00 0 1 1.57556 -0.34
5 328 1w5q (A) 9 333 48.00 0 1 1.5614 -0.56
8 328 3obk (A) 20 341 44.00 0 1 1.53328 -0.64
9 326 1l6s (A) 5 318 48.00 0 1 1.58047 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127486)

Species Accession Gene Product
Arabidopsis thaliana AT1G69740   putative delta-aminolevulinic acid dehydratase
Babesia bovis BBOV_II001120   delta-aminolevulinic acid dehydratase, putative
Candida albicans CaO19.8517   porphobilinogen synthase
Candida albicans CaO19.898   porphobilinogen synthase
Chlamydia trachomatis CT_633   delta-aminolevulinic acid dehydratase
Dictyostelium discoideum DDB_G0269444   delta-aminolevulinate dehydratase
Drosophila melanogaster Dmel_CG10335   Porphobilinogen synthase
Escherichia coli b0369   5-aminolevulinate dehydratase (porphobilinogen synthase)
Echinococcus granulosus EgrG_000471300   delta aminolevulinic acid dehydratase
Echinococcus multilocularis EmuJ_000471300   delta aminolevulinic acid dehydratase
Homo sapiens ENSG00000148218   aminolevulinate dehydratase
Mycobacterium leprae ML2419c   PROBABLE DELTA-AMINOLEVULINIC ACID DEHYDRATASE HEMB (PORPHOBILINOGEN SYNTHASE) (ALAD) (ALADH)
Mus musculus ENSMUSG00000028393   aminolevulinate, delta-, dehydratase
Mycobacterium tuberculosis Rv0512   Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH)
Mycobacterium ulcerans MUL_4601   delta-aminolevulinic acid dehydratase
Neospora caninum NCLIV_008230   Delta-aminolevulinic acid dehydratase (EC 4.2.1.24), related
Oryza sativa 4341997   Os06g0704600
Plasmodium berghei PBANKA_1304100   porphobilinogen synthase, putative
Plasmodium falciparum PF3D7_1440300   porphobilinogen synthase
Plasmodium knowlesi PKNH_1242100   porphobilinogen synthase, putative
Plasmodium vivax PVX_118480   delta-aminolevulinic acid dehydratase, putative
Plasmodium yoelii PY04302   porphobilinogen synthase, putative
Saccharomyces cerevisiae YGL040C   porphobilinogen synthase HEM2
Schistosoma japonicum Sjp_0075570   ko:K01698 porphobilinogen synthase [EC4.2.1.24], putative
Schistosoma mansoni Smp_045810.1   porphobilinogen synthase
Schistosoma mansoni Smp_045810.2   porphobilinogen synthase
Schmidtea mediterranea mk4.029516.02  
Schmidtea mediterranea mk4.000123.05   Delta-aminolevulinic acid dehydratase
Toxoplasma gondii TGME49_253900   parasite porphobilinogen synthase PBGS
Wolbachia endosymbiont of Brugia malayi Wbm0373   delta-aminolevulinic acid dehydratase

Essentiality

Rv0512 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0369 Escherichia coli essential goodall
YGL040C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1304100 Plasmodium berghei Essential plasmo
TGME49_253900 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228) Delta-aminolevulinic acid dehydratase Compounds References
Wolbachia sp. subsp. Brugia malayi (strain TRS) Delta-aminolevulinic acid dehydratase Compounds References
Escherichia coli 5-aminolevulinate dehydratase (porphobilinogen synthase) Compounds References
Pisum sativum Delta-aminolevulinic acid dehydratase, chloroplastic Compounds References
Plasmodium falciparum porphobilinogen synthase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.023 0.5006 0.5
0.0121 0.3591 0
0.0116 0.3387 0.638
0.0244 0.4945 0.5
0.0246 0.3222 0.5
0.0211 0.4903 0.5
0.0227 0.2629 1
0.0235 0.2559 1
0.0227 0.2629 1
0.0246 0.3222 0.5
0.0503 0.3415 1
0.0227 0.2629 1
0.0116 0.3621 0.638
0.0132 0.4059 1
0.0503 0.3415 1
0.0227 0.2629 1
0.0115 0.2924 0
0.0227 0.2629 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv0512 (Mycobacterium tuberculosis), Probable delta-aminolevulinic acid dehydratase HemB (porphobilinogen synthase) (ALAD) (ALADH)
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