Detailed view for Rv1695

Basic information

TDR Targets ID: 981257
Mycobacterium tuberculosis, Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase)
Source Database / ID:  Tuberculist 

pI: 5.1004 | Length (AA): 307 | MW (Da): 32872 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Nicotinate and nicotinamide metabolism (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 305 1u0r (D) 2 305 99.99 0 1 2.15573 -0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127041)

Species Accession Gene Product
Arabidopsis thaliana AT3G21070   NAD(H) kinase 1
Arabidopsis thaliana AT1G21640   NAD kinase 2
Babesia bovis BBOV_IV002800   ATP-NAD-dependent kinase, putative
Candida albicans CaO19.7393   NAD kinase associated with ferric reductase
Cryptosporidium hominis Chro.80231   inorganic polyphosphate/ATP-NAD kinase
Cryptosporidium parvum cgd8_1970   NAD kinase involved in polyphosphate metabolism
Dictyostelium discoideum DDB_G0272224   NAD+ kinase family protein
Dictyostelium discoideum DDB_G0292442   NAD+ kinase family protein
Drosophila melanogaster Dmel_CG33156   CG33156 gene product from transcript CG33156-RF
Drosophila melanogaster Dmel_CG6145   CG6145 gene product from transcript CG6145-RE
Escherichia coli b2615   NAD kinase
Echinococcus granulosus EgrG_000193300   NAD kinase
Echinococcus multilocularis EmuJ_000193300   NAD kinase
Giardia lamblia GL50803_17316   Inorganic polyphosphate/ATP-NAD kinase, putative
Homo sapiens ENSG00000008130   NAD kinase
Mycobacterium leprae ML1359   Inorganic polyphosphate/ATP-NAD kinase PpnK (Poly(P)/ATP NAD kinase)
Mus musculus ENSMUSG00000029063   NAD kinase
Mycobacterium tuberculosis Rv1695   Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase)
Mycobacterium ulcerans MUL_1684   inorganic polyphosphate/ATP-NAD kinase
Neospora caninum NCLIV_019250   ATP-NAD/AcoX kinase, related
Oryza sativa 4349979   Os11g0191400
Oryza sativa 4325416   Os01g0957000
Oryza sativa 9266739   Os05g0388400
Saccharomyces cerevisiae YEL041W   Yef1p
Saccharomyces cerevisiae YJR049C   Utr1p
Schistosoma japonicum Sjp_0065130   ko:K00858 NAD+ kinase [EC2.7.1.23], putative
Schistosoma mansoni Smp_094870   poly(p)/ATP NAD kinase
Schmidtea mediterranea mk4.064899.00  
Schmidtea mediterranea mk4.024641.00  
Schmidtea mediterranea mk4.001685.02  
Treponema pallidum TP0441   hypothetical protein
Theileria parva TP03_0366   hypothetical protein, conserved
Trichomonas vaginalis TVAG_485920   poly(p)/ATP NAD kinase, putative
Trichomonas vaginalis TVAG_056970   poly(p)/ATP NAD kinase, putative

Essentiality

Rv1695 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b2615 Escherichia coli essential goodall
YJR049C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
References
References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Staphylococcus aureus (strain COL) NAD kinase Compounds References
Homo sapiens NAD kinase Compounds References
Listeria monocytogenes serotype 4b (strain F2365) NAD kinase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0315 0.3519 0.8579
0.037 0.9107 1
0.0324 0.3174 1
0.0383 0.4241 0.5
0.0136 0.5414 1
0.0319 0.4023 1
0.0319 0.4023 1
0.0315 0.3519 0.8579
0.0325 0.8909 1
0.0206 0.3769 1
0.037 0.9107 1
0.0325 0.8909 1
0.0322 0.8924 1
0.0304 0.573 1
0.0329 0.5081 1
0.0383 0.4241 0.5
0.0189 0.4179 1
0.0315 0.3519 0.8579
0.0306 0.573 1

Assayability

Assay information

  • Inhibition of Mycobacterium tuberculosis NAD kinase at 1 mM ChEMBL
  • Inhibition of Mycobacterium tuberculosis NAD kinase at 1 mM
  • Inhibition of Mycobacterium tuberculosis NAD kinase at 2 mM ChEMBL
  • Inhibition of Mycobacterium tuberculosis NAD kinase at 2 mM
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis NAD kinase by modified HPLC based assay

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1695 (Mycobacterium tuberculosis), Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase)
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