Detailed view for Rv2845c

Basic information

TDR Targets ID: 980523
Mycobacterium tuberculosis, Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase)
Source Database / ID:  Tuberculist 

pI: 4.8564 | Length (AA): 582 | MW (Da): 63270 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 570 2j3l (A) 1 556 42.00 0 1 1.49725 -0.34
4 581 5ucm (A) 2 570 41.00 0 1 1.47243 -0.12
234 405 2z0x (A) 2 158 13.00 0 0.66 0.390933 -0.41
259 404 1dbu (A) 19 157 14.00 0 0.56 0.373259 -0.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128299)

Species Accession Gene Product
Babesia bovis BBOV_III011010   tRNA synthetase class II core domain containing protein
Brugia malayi Bm1_53720   Met-10+ like-protein
Brugia malayi Bm1_47665   probable proline-trna synthetase - fission yeast, putative
Caenorhabditis elegans CELE_T27F6.5   Protein PARS-2, isoform D
Chlamydia trachomatis CT_393   proline--tRNA ligase
Dictyostelium discoideum DDB_G0276079   proline-tRNA ligase
Drosophila melanogaster Dmel_CG12186   Prolyl-tRNA synthetase
Escherichia coli b0194   prolyl-tRNA synthetase
Echinococcus granulosus EgrG_000461800   prolyl tRNA synthetase mitochondrial
Echinococcus multilocularis EmuJ_000461800   prolyl tRNA synthetase, mitochondrial
Homo sapiens ENSG00000162396   prolyl-tRNA synthetase 2, mitochondrial (putative)
Loa Loa (eye worm) LOAG_05904   hypothetical protein
Mus musculus ENSMUSG00000043572   prolyl-tRNA synthetase (mitochondrial)(putative)
Mycobacterium tuberculosis Rv2845c   Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase)
Mycobacterium ulcerans MUL_2116   prolyl-tRNA synthetase
Plasmodium berghei PBANKA_0826100   proline--tRNA ligase, putative
Plasmodium falciparum PF3D7_0925300   proline--tRNA ligase, putative
Plasmodium knowlesi PKNH_0723200   proline--tRNA ligase, putative
Plasmodium vivax PVX_099680   proline--tRNA ligase, putative
Plasmodium yoelii PY00927   prolyl-tRNA synthetase-related
Saccharomyces cerevisiae YER087W   putative proline--tRNA ligase AIM10
Schistosoma japonicum Sjp_0306460   Probable prolyl-tRNA synthetase, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0117790   ko:K01881 prolyl-tRNA synthetase [EC6.1.1.15], putative
Schistosoma mansoni Smp_025260   prolyl-tRNA synthetase
Schmidtea mediterranea mk4.000424.07  
Schmidtea mediterranea mk4.000424.06  
Schmidtea mediterranea mk4.000424.05  
Treponema pallidum TP0160   prolyl-tRNA synthetase
Theileria parva TP02_0040   prolyl-tRNA synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0077   prolyl-tRNA synthetase

Essentiality

Rv2845c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0194 Escherichia coli essential goodall
CELE_T27F6.5 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Candida albicans Prolyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0225 0.7229 1
0.0259 0.7305 1
0.0143 0.4202 0.3315
0.0087 0.3535 1
0.0195 0.7121 1
0.0532 0.2739 1
0.0264 0.3262 0.808
0.0536 0.7318 1
0.0055 0.5232 0.5
0.026 0.7273 1
0.0263 0.7304 0.7538
0.0532 0.2739 1
0.0163 0.3928 1
0.0108 0.6125 1
0.0174 0.7064 1
0.0251 0.7187 0.6612
0.023 0.2764 1
0.0034 0.5695 1
0.0176 0.6941 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2845c (Mycobacterium tuberculosis), Probable prolyl-tRNA synthetase ProS (proline--tRNA ligase) (PRORS) (global RNA synthesis factor) (proline translase)
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