Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | cytochrome P450, family 3, subfamily A, polypeptide 4 | Starlite/ChEMBL | References |
Rattus norvegicus | Metabotropic glutamate receptor 5 | Starlite/ChEMBL | References |
Homo sapiens | glutamate receptor, metabotropic 5 | Starlite/ChEMBL | No references |
Species | Potential target | Known druggable target | Length | Alignment span | Identity |
---|---|---|---|---|---|
Brugia malayi | cytochrome P450 | cytochrome P450, family 3, subfamily A, polypeptide 4 | 502 aa | 492 aa | 24.2 % |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Schistosoma mansoni | metabotropic glutamate receptor | 0.0048 | 0.3042 | 0.3335 |
Onchocerca volvulus | Metabotropic glutamate receptor homolog | 0.0016 | 0 | 0.5 |
Loa Loa (eye worm) | metabotropic GABA-B receptor subtype 2 | 0.0025 | 0.088 | 0.088 |
Echinococcus granulosus | metabotropic glutamate receptor 2 | 0.0082 | 0.6319 | 0.6319 |
Brugia malayi | Receptor family ligand binding region containing protein | 0.0025 | 0.088 | 0.1122 |
Brugia malayi | Metabotropic glutamate receptor precursor. | 0.0098 | 0.7838 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.012 | 1 | 1 |
Brugia malayi | metabotropic glutamate receptor subtype 5a (mGluR5a), putative | 0.0089 | 0.6958 | 0.8878 |
Loa Loa (eye worm) | glutamate receptor | 0.0038 | 0.2162 | 0.2162 |
Brugia malayi | metabotropic glutamate receptor type 2 | 0.0048 | 0.3042 | 0.3881 |
Echinococcus multilocularis | metabotropic glutamate receptor 5 | 0.012 | 1 | 1 |
Schistosoma mansoni | metabotropic glutamate receptor 2 3 (mglur group 2) | 0.0111 | 0.912 | 1 |
Loa Loa (eye worm) | glutamate receptor | 0.0098 | 0.7838 | 0.7838 |
Loa Loa (eye worm) | receptor family ligand binding region containing protein | 0.0025 | 0.088 | 0.088 |
Schistosoma mansoni | metabotropic glutamate receptor | 0.0082 | 0.6319 | 0.6929 |
Echinococcus multilocularis | metabotropic glutamate receptor 2 | 0.0082 | 0.6319 | 0.6319 |
Loa Loa (eye worm) | hypothetical protein | 0.0025 | 0.088 | 0.088 |
Onchocerca volvulus | Poor gastrulation protein homolog | 0.0016 | 0 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
%max (binding) | = 1.4 % | Negative allosteric modulation of rat mGlu5 receptor expressed in HEK293A cells assessed as inhibition of glutamate-induced calcium mobilization at 30 uM preincubated for 140 seconds prior to glutamate-stimulation measured after 60 seconds relative to glutamate | ChEMBL. | 24074843 |
IC50 (binding) | = 7.94 | Negative allosteric modulation of rat mGlu5 receptor expressed in HEK293A cells assessed as inhibition of glutamate-induced calcium mobilization preincubated for 140 seconds prior to glutamate-stimulation measured after 60 seconds | ChEMBL. | 24074843 |
IC50 (binding) | = 11 nM | Negative allosteric modulation of rat mGlu5 receptor expressed in HEK293A cells assessed as inhibition of glutamate-induced calcium mobilization preincubated for 140 seconds prior to glutamate-stimulation measured after 60 seconds | ChEMBL. | 24074843 |
IC50 (binding) | = 11 nM | BindingDB_Patents: Metabotropic Glutamate Receptor Activity Assay. The utility of the compounds in accordance with the present invention as negative allosteric modulators of metabotropic glutamate receptor activity, in particular mGluR5 activity, can be demonstrated by methodology known in the art. Human embryonic kidney (HEK) cells transfected with rat or human mGluR5 were plated in clear bottom assay plates for assay in a Functional Drug Screening System (FDSS). The cells were loaded with a Ca2+-sensitive fluorescent dye (e.g., Fluo-4), and the plates were washed and placed in the FDSS instrument. Test compound was applied to cells 3 seconds after baseline readings were taken. Cells were incubated with the test compounds for 140 seconds and then stimulated with an EC20 concentration of an mGluR5 agonist (e.g., glutamate, 3,5-dihydroxyphenylglycine, or quisqualate); 60-80 seconds later an EC80 concentration of agonist was added and readings taken for an additional 40 seconds. Data were collected at 1 Hz. Negative allosteric modulation of the agonist. | ChEMBL. | No reference |
IC50 (binding) | = 16 nM | Metabotropic Glutamate Receptor Activity Assay | BINDINGDB. | No reference |
IC50 (ADMET) | = 2.2 uM | Inhibition of CYP3A4 in human liver microsomes in presence of NADPH | ChEMBL. | 24074843 |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.
1 literature reference was collected for this gene.