Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | mitogen-activated protein kinase kinase kinase 5 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | thioredoxin glutathione reductase | 0.0446 | 1 | 1 |
Schistosoma mansoni | protein kinase | 0.0249 | 0.4691 | 0.3249 |
Brugia malayi | Thioredoxin reductase | 0.0446 | 1 | 1 |
Plasmodium falciparum | glutathione reductase | 0.0446 | 1 | 1 |
Echinococcus multilocularis | thioredoxin glutathione reductase | 0.0446 | 1 | 1 |
Wolbachia endosymbiont of Brugia malayi | dihydrolipoamide dehydrogenase E3 component | 0.0155 | 0.2135 | 0.5 |
Plasmodium vivax | glutathione reductase, putative | 0.0446 | 1 | 1 |
Trichomonas vaginalis | mercuric reductase, putative | 0.0155 | 0.2135 | 0.5 |
Trypanosoma cruzi | dihydrolipoyl dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Trypanosoma cruzi | dihydrolipoamide dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Brugia malayi | dihydrolipoyl dehydrogenase, mitochondrial precursor, putative | 0.0155 | 0.2135 | 0.1234 |
Trypanosoma brucei | trypanothione reductase | 0.0446 | 1 | 1 |
Trypanosoma cruzi | trypanothione reductase, putative | 0.0155 | 0.2135 | 0.2135 |
Loa Loa (eye worm) | hypothetical protein | 0.0249 | 0.4681 | 0.4681 |
Mycobacterium tuberculosis | NADPH-dependent mycothiol reductase Mtr | 0.0446 | 1 | 1 |
Trypanosoma cruzi | dihydrolipoamide dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Plasmodium falciparum | thioredoxin reductase | 0.0446 | 1 | 1 |
Brugia malayi | Neuronal symmetry protein 1 | 0.0249 | 0.4691 | 0.4082 |
Echinococcus multilocularis | mitogen activated protein kinase kinase kinase | 0.0249 | 0.4691 | 0.3249 |
Loa Loa (eye worm) | glutathione reductase | 0.0446 | 1 | 1 |
Trypanosoma cruzi | dihydrolipoamide dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Echinococcus granulosus | mitogen activated protein kinase kinase kinase | 0.0249 | 0.4691 | 0.3249 |
Giardia lamblia | NADH oxidase lateral transfer candidate | 0.0155 | 0.2135 | 0.5 |
Toxoplasma gondii | thioredoxin reductase | 0.0446 | 1 | 1 |
Chlamydia trachomatis | dihydrolipoyl dehydrogenase | 0.0155 | 0.2135 | 0.5 |
Leishmania major | trypanothione reductase | 0.0446 | 1 | 1 |
Trypanosoma cruzi | dihydrolipoamide dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Treponema pallidum | NADH oxidase | 0.0155 | 0.2135 | 0.5 |
Mycobacterium ulcerans | dihydrolipoamide dehydrogenase | 0.0155 | 0.2135 | 0.5 |
Schistosoma mansoni | protein kinase | 0.0249 | 0.4691 | 0.3249 |
Plasmodium vivax | thioredoxin reductase, putative | 0.0446 | 1 | 1 |
Mycobacterium ulcerans | flavoprotein disulfide reductase | 0.0155 | 0.2135 | 0.5 |
Trypanosoma cruzi | dihydrolipoyl dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Trypanosoma cruzi | dihydrolipoamide dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Mycobacterium leprae | DIHYDROLIPOAMIDE DEHYDROGENASE LPD (LIPOAMIDE REDUCTASE (NADH)) (LIPOYL DEHYDROGENASE) (DIHYDROLIPOYL DEHYDROGENASE) (DIAPHORASE | 0.0155 | 0.2135 | 0.5 |
Wolbachia endosymbiont of Brugia malayi | dihydrolipoamide dehydrogenase E3 component | 0.0155 | 0.2135 | 0.5 |
Loa Loa (eye worm) | thioredoxin reductase | 0.0446 | 1 | 1 |
Trichomonas vaginalis | glutathione reductase, putative | 0.0155 | 0.2135 | 0.5 |
Trypanosoma cruzi | dihydrolipoamide dehydrogenase, putative | 0.0155 | 0.2135 | 0.2135 |
Mycobacterium ulcerans | dihydrolipoamide dehydrogenase, LpdB | 0.0155 | 0.2135 | 0.5 |
Trypanosoma cruzi | trypanothione reductase, putative | 0.0446 | 1 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 2 nM | BindingDB_Patents: Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay. The assay measures the phosphorylation level of a biotinylated peptide substrate by the ASK1 kinase using HTRF detection (6.1). This is a competitive, time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay, based on HTRF KinEASE-STK manual from Cisbio (6.1). Test compound, 1 uM STK3 peptide substrate, 4 nM of ASK1 kinase are incubated with 10 mM MOP buffer, pH. 7.0 containing 10 mM Mg-acetate, 0.025% NP-40, 1 mM DTT, 0.05% BSA and 1.5% glycerol for 30 minutes then 100 uM ATP is added to start the kinase reaction and incubated for 3 hr. Peptide antibody labeled with 1x Eu3+ Cryptate buffer containing 10 mM EDTA and 125 nM Streptavidin XL665 are added to stop the reaction and phosphorylated peptide substrate is detected using Envision 2103 Multilabeled reader from PerkinElmer. The fluorescence is measured at 615 nm (Cryptate) and 665 nm (XL665) and a ratio of 665 nm/615 nm is calculated for each well. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.