Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | meningioma expressed antigen 5 (hyaluronidase) | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus multilocularis | bifunctional protein NCOAT | 0.0327 | 0.3366 | 0.3366 |
Brugia malayi | Hyaluronidase family protein | 0.0327 | 0.3366 | 1 |
Schistosoma mansoni | hypothetical protein | 0.0186 | 0.1705 | 0.5065 |
Entamoeba histolytica | hypothetical protein | 0.0041 | 0 | 0.5 |
Echinococcus granulosus | geminin | 0.0186 | 0.1705 | 0.5065 |
Entamoeba histolytica | hypothetical protein | 0.0041 | 0 | 0.5 |
Schistosoma mansoni | aminopeptidase P homologue (M24 family) | 0.0327 | 0.3366 | 1 |
Brugia malayi | MH2 domain containing protein | 0.0268 | 0.2671 | 0.7935 |
Entamoeba histolytica | hypothetical protein | 0.0041 | 0 | 0.5 |
Schistosoma mansoni | hypothetical protein | 0.0186 | 0.1705 | 0.5065 |
Echinococcus multilocularis | geminin | 0.0186 | 0.1705 | 0.1705 |
Entamoeba histolytica | hypothetical protein | 0.0041 | 0 | 0.5 |
Loa Loa (eye worm) | MH2 domain-containing protein | 0.0268 | 0.2671 | 0.6863 |
Loa Loa (eye worm) | transcription factor SMAD2 | 0.0268 | 0.2671 | 0.6863 |
Loa Loa (eye worm) | hyaluronidase | 0.0327 | 0.3366 | 1 |
Echinococcus granulosus | bifunctional protein NCOAT | 0.0327 | 0.3366 | 1 |
Schistosoma mansoni | Hyaluronidase | 0.0327 | 0.3366 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
Ki (binding) | = 9.36 nM | BindingDB_Patents: Inhibition Assay. Enzymatic reactions are carried out in a reaction containing 50 mM NaH2PO4, 100 mM NaCl and 0.1% BSA (pH 7.0) using 2 mM 4-Methylumbelliferyl N-acetyl-ß-D-glucosaminide dihydrate (Sigma M2133) dissolved in ddH2O, as a substrate. The amount of purified human O-GlcNAcase enzyme used in the reaction is 0.7 nM. Test compound of varying concentrations is added to the enzyme prior to initiation of the reaction. The reaction is performed at room temperature in a 96-well plate and is initiated with the addition of substrate. The production of fluorescent product is measured every 60 sec for 45 min with a Tecan Infinite M200 plate-reader with excitation at 355 nM and emission detected at 460 nM, with 4-Methylumbelliferone (Sigma M1381) used to produce a standard curve. The slope of product production is determined for each concentration of compound tested and plotted, using standard curve fitting algorithms for sigmoidal dose response curves. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.