Detailed view for Tb927.7.5820

Basic information

TDR Targets ID: 15125
Trypanosoma brucei, Monooxygenase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9697 | Length (AA): 515 | MW (Da): 55087 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01494   FAD binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0071949   GO:FAD binding  

GO:0004497   monooxygenase activity  
GO:0016491   oxidoreductase activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128116)

Species Accession Gene Product
Arabidopsis thaliana AT3G24200   FAD/NAD(P)-binding oxidoreductase family protein
Candida albicans CaO19.3058   COQ6 monooxygenase, coenzyme Q biosynthesis
Candida albicans CaO19.10576   COQ6 monooxygenase, coenzyme Q biosynthesis
Caenorhabditis elegans CELE_K07B1.2   Protein COQ-6
Dictyostelium discoideum DDB_G0291440   hypothetical protein
Drosophila melanogaster Dmel_CG7277   CG7277 gene product from transcript CG7277-RA
Escherichia coli b0662   2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol oxygenase
Escherichia coli b2906   2-octaprenylphenol hydroxylase, FAD-dependent
Echinococcus granulosus EgrG_000422500   ubiquinone biosynthesis monooxygenase COQ6
Echinococcus multilocularis EmuJ_000422500   ubiquinone biosynthesis monooxygenase COQ6
Homo sapiens ENSG00000119723   coenzyme Q6 monooxygenase
Leishmania braziliensis LbrM.06.1230   hypothetical protein, conserved
Leishmania donovani LdBPK_061290.1   FAD binding domain containing protein, putative
Leishmania infantum LinJ.06.1290   hypothetical protein, conserved
Leishmania major LmjF.06.1240   hypothetical protein, conserved
Leishmania mexicana LmxM.06.1240   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_10177   hypothetical protein
Mus musculus ENSMUSG00000021235   coenzyme Q6 homolog (yeast)
Oryza sativa 4334721   Os03g0839100
Saccharomyces cerevisiae YGR255C   putative N,N-dimethylaniline monooxygenase COQ6
Schistosoma japonicum Sjp_0010040   ko:K06126 ubiquinone biosynthesis monooxygenase Coq6, putative
Schistosoma mansoni Smp_153460   monoxygenase
Schmidtea mediterranea mk4.003012.01   Probable ubiquinone biosynthesis monooxygenase coq-6
Schmidtea mediterranea mk4.003012.00   Probable ubiquinone biosynthesis monooxygenase coq-6
Trypanosoma brucei gambiense Tbg972.7.6780   Monooxygenase, putative
Trypanosoma brucei Tb927.7.5820   Monooxygenase, putative
Trypanosoma congolense TcIL3000_7_4820   Monooxygenase, putative
Trypanosoma cruzi TcCLB.508173.100   Monooxygenase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0064   2-polyprenyl-6-methoxyphenol 4-hydroxylase

Essentiality

Tb927.7.5820 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5820 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.5820 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.5820 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.5820 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0662 Escherichia coli essential goodall
b2906 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0219 1 0.5
0.0063 0.627 0.5
0.0171 0.5742 1
0.0086 1 1
0.0196 1 1
0.0093 1 1
0.0113 0.5252 0.5
0.0213 1 1
0.0214 0.991 0.991
0.0066 1 1
0.0116 1 0.5
0.0271 1 1
0.0219 1 1
0.0111 1 0.5
0.0143 0.3221 0.3787
0.015 1 1
0.0138 0.4448 0.5
0.0079 0.5 0.5
0.0139 0.3178 1
0.0353 0.5761 0.5
0.0219 1 0.5
0.0171 0.5742 1
0.0671 1 1
0.0095 0.5 0.5
0.0146 0.6701 0.6701
0.0171 0.5742 1
0.0176 0.7425 1
0.0224 0.3826 0.5
0.0206 1 1
0.0018 0.3365 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.7.5820 (Trypanosoma brucei), Monooxygenase, putative
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