Detailed view for TGME49_215390

Basic information

TDR Targets ID: 260653
Toxoplasma gondii, TIM10 family protein, putative
Source Database / ID:  ToxoDB 

pI: 4.5237 | Length (AA): 77 | MW (Da): 8580 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02953   Tim10/DDP family zinc finger

Gene Ontology

Mouse over links to read term descriptions.
GO:0042721   mitochondrial inner membrane protein insertion complex  
GO:0042719   mitochondrial intermembrane space protein transporter complex  
GO:0008565   protein transporter activity  
GO:0045039   protein import into mitochondrial inner membrane  
GO:0006626   protein targeting to mitochondrion  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 77 2bsk (D) 1 78 42.00 0 0.86 1.5237 -0.38
20 66 3cjh (A) 48 90 30.00 0.026 0.69 0.96749 -0.84
22 57 2bsk (A) 21 56 31.00 0.0058 0.58 0.919632 -0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128839)

Species Accession Gene Product
Arabidopsis thaliana AT2G29530   mitochondrial import inner membrane translocase subunit Tim10
Babesia bovis BBOV_IV008900   mitochondrial transport complex Tim10, putative
Brugia malayi Bm1_18555   Mitochondrial import inner membrane translocase subunit TIM10
Candida albicans CaO19.12090   protein trans. across mito. Intermembrane space
Candida albicans CaO19.4620   protein trans. across mito. Intermembrane space
Caenorhabditis elegans CELE_Y66D12A.22   Protein TIN-10
Dictyostelium discoideum DDB_G0284911   mitochondrial import inner membrane translocase subunit 10
Drosophila melanogaster Dmel_CG9878   Translocase of inner membrane 10
Echinococcus granulosus EgrG_000524500   expressed protein
Echinococcus multilocularis EmuJ_000524500   expressed protein
Homo sapiens ENSG00000134809   translocase of inner mitochondrial membrane 10 homolog (yeast)
Loa Loa (eye worm) LOAG_11210   transport to inner mitochondrial membrane family member
Mus musculus ENSMUSG00000027076   translocase of inner mitochondrial membrane 10
Neospora caninum NCLIV_026310   mitochondrial import inner membrane translocase subunit tim10, putative
Neospora caninum NCLIV_052310   Tim10/DDP family zinc finger containing protein, putative
Oryza sativa 4334631   Os03g0825400
Oryza sativa 4342818   Os07g0243100
Plasmodium berghei PBANKA_0607100   mitochondrial import inner membrane translocase subunit TIM10, putative
Plasmodium falciparum PF3D7_1208600   mitochondrial import inner membrane translocase subunit TIM10, putative
Plasmodium knowlesi PKNH_1308500   mitochondrial import inner membrane translocase subunit TIM10, putative
Plasmodium vivax PVX_084462   mitochondrial import inner membrane translocase subunit TIM10, putative
Plasmodium yoelii PY06161   small zinc finger-like protein-related
Saccharomyces cerevisiae YBR091C   Tim12p
Saccharomyces cerevisiae YHR005C-A   protein transporter TIM10
Schistosoma japonicum Sjp_0036650   Mitochondrial import inner membrane translocase subunit Tim10, putative
Schistosoma mansoni Smp_016130   mitochondrial import inner membrane translocase subunit tim10
Toxoplasma gondii TGME49_260850   mitochondrial import inner membrane translocase subunit TIM10, putative
Toxoplasma gondii TGME49_215390   TIM10 family protein, putative
Theileria parva TP01_0885   mitochondrial import inner membrane translocase subunit tim10, putative

Essentiality

TGME49_215390 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_Y66D12A.22 Caenorhabditis elegans embryonic lethal wormbase
YHR005C-A Saccharomyces cerevisiae inviable yeastgenome
YBR091C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0607100 Plasmodium berghei Essential plasmo
TGME49_260850 Toxoplasma gondii Essentiality uncertain sidik
TGME49_215390 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_260850 Toxoplasma gondii Probably non-essential sidik
TGME49_215390 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Saccharomyces cerevisiae protein transporter TIM10 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.005 0.3584 0.3405
0.0058 1 1
0.0086 0.3133 0.1827
0.0058 0.3109 1
0.0051 0.2712 0.7039
0.0058 0.7706 0.7677
0.0046 0.2803 1
0.0097 0.2783 1
0.0036 1 1
0.0049 0.3551 0.3818
0.0051 0.2712 0.7039
0.0058 0.4538 1
0.0107 0.4273 0.781
0.0058 0.4366 1
0.0058 0.2554 1
0.0058 0.2803 1
0.005 1 1
0.0058 0.2776 0.7073
0.0047 0.6477 1
0.0058 0.3682 1
0.0058 0.3444 1
0.0058 0.3299 1
0.0102 0.6634 0.785
0.0102 0.6634 0.785
0.0103 0.2968 1
0.0049 0.9382 0.5
0.0097 0.3255 1
0.0048 0.3012 1
0.0057 1 1
0.0058 0.2584 0.5
0.0058 0.8664 0.5
0.0051 0.2712 0.7039
0.0058 0.3764 0.3572
0.0058 0.93 0.9714
0.0086 0.3133 0.1827
0.0058 0.9473 1
0.0058 0.6881 1
0.0058 1 1
0.0058 0.3764 1
0.0048 0.2803 1
0.0058 0.4538 1
0.0058 0.9382 0.5
0.0105 0.4812 0.7855
0.0058 0.2803 1
0.0058 0.2584 0
0.0058 0.3718 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_215390 (Toxoplasma gondii), TIM10 family protein, putative
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