Detailed view for TGME49_273500

Basic information

TDR Targets ID: 265274
Toxoplasma gondii, O-linked N-acetylglucosamine transferase
Source Database / ID:  ToxoDB 

pI: 7.3851 | Length (AA): 978 | MW (Da): 108158 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00515   Tetratricopeptide repeat
PF13374   Tetratricopeptide repeat
PF13431   Tetratricopeptide repeat
PF13844   Glycosyl transferase family 41

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 16 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
49 219 1fch (A) 346 532 23.00 0.000036 0.89 0.41 0.31
66 229 2ho1 (A) 32 198 17.00 0.000019 0.38 0.34 -0.04
101 277 1xnf (A) 27 201 18.00 0.000016 0.52 0.4 -0.06
142 541 1w3b (A) 15 400 25.00 0 1 0.78 -0.43
424 528 1na0 (A) 9 113 41.00 0 1 0.79 -1.74
729 914 2bfw (A) 234 413 12.00 0 0.6 0.34 -0.89
13 181 5cwm (A) 3 174 16.00 0.59 0.13 0.311502 -0.37
17 247 3cvp (A) 352 622 21.00 0.003 0.97 0.442896 0.19
136 205 1elw (A) 2 71 17.00 0.12 0.54 0.358275 -0.74
156 539 4j0u (A) 33 480 16.00 0 1 0.332338 0.74
191 265 1p5q (A) 338 412 31.00 0.31 1 0.620387 -1.46
247 347 4cgv (A) 133 233 34.00 0.000074 0.95 0.411972 -0.26
278 976 4gyw (A) 314 1019 18.00 0 1 0.936424 0.13
278 342 2if4 (A) 227 291 17.00 0 0.46 0.339162 -0.83
391 421 2kc7 (A) 36 66 52.00 0.31 0.93 0.616397 0.16
437 524 3sz7 (A) 127 214 30.00 0.00055 1 0.62168 -1.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127266)

Species Accession Gene Product
Arabidopsis thaliana AT3G04240   putative UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC
Arabidopsis thaliana AT3G11540   putative UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY
Brugia malayi Bm1_01735   UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase
Caenorhabditis elegans CELE_K04G7.3   Protein OGT-1, isoform B
Cryptosporidium hominis Chro.10151   SPY protein
Cryptosporidium parvum cgd1_1300   spindly like TPR repeats, predicted plant origin
Chlamydia trachomatis CT_683   hypothetical protein
Dictyostelium discoideum DDB_G0285263   O-linked GlcNAc transferase
Drosophila melanogaster Dmel_CG10392   super sex combs
Echinococcus granulosus EgrG_000741100   O glycosyltransferase
Echinococcus multilocularis EmuJ_000741100   O glycosyltransferase
Giardia lamblia GL50803_12081   O-linked GlcNAc transferase
Homo sapiens ENSG00000147162   O-linked N-acetylglucosamine (GlcNAc) transferase
Loa Loa (eye worm) LOAG_03076   UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase
Mus musculus ENSMUSG00000034160   O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Mycobacterium ulcerans MUL_2398   hypothetical protein
Neospora caninum NCLIV_034150   hypothetical protein
Oryza sativa 4346315   Os08g0559300
Schistosoma japonicum Sjp_0041880   ko:K09667 polypeptide N-acetylglucosaminyltransferase, putative
Schistosoma mansoni Smp_046930   o-linked n-acetylglucosamine transferase ogt
Schmidtea mediterranea mk4.009717.03   UDP-N-acetylglucosamineALQ-peptide N-acetylglucosaminyltransferase
Toxoplasma gondii TGME49_273500   O-linked N-acetylglucosamine transferase
Treponema pallidum TP0468   hypothetical protein
Treponema pallidum TP0067   hypothetical protein
Treponema pallidum TP0648   hypothetical protein
Treponema pallidum TP0954   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0526   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0525   hypothetical protein

Essentiality

TGME49_273500 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_273500 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens O-linked N-acetylglucosamine (GlcNAc) transferase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0331 0.3855 1
0.0206 0.2577 1
0.0387 0.4072 1
0.0382 0.4087 1
0.0389 0.3649 1
0.0338 0.28 1
0.035 0.3677 1
0.0205 0.5993 1
0.0124 0.2621 1
0.0439 0.3556 1
0.0378 0.5162 1
0.0442 0.4042 1
0.0122 0.2629 1
0.0336 0.4484 1
0.0039 0.2643 0.8562
0.0294 0.3131 1
0.0072 0.5303 1
0.0338 0.3243 1
0.0334 0.4053 1
0.004 0.8128 1
0.0428 0.3007 1
0.0382 0.4073 1
0.0386 0.3964 1
0.0039 0.2636 0.8562
0.00540778 0.672982 1
0.0351 0.9887 1
0.0392 0.3927 1
0.0387 0.4067 1
0.0039 0.2551 0.8562
0.0331 0.345 1
0.0406 0.3165 1
0.0043 0.8128 1
0.0337 0.461 1
0.0206 0.2922 1
0.0386 0.5174 1
0.0341 0.3643 1
0.0401 0.3282 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

9 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_273500 (Toxoplasma gondii), O-linked N-acetylglucosamine transferase
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