Detailed view for PF3D7_0919600

Basic information

TDR Targets ID: 4871
Plasmodium falciparum, dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 10.0507 | Length (AA): 477 | MW (Da): 57008 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03345   Oligosaccharyltransferase 48 kDa subunit beta

Gene Ontology

Mouse over links to read term descriptions.
GO:0005789   endoplasmic reticulum membrane  
GO:0004579   dolichyl-diphosphooligosaccharide-protein glycotransferase activity  
GO:0018279   protein amino acid N-linked glycosylation via asparagine  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
45 219 4u5a (C) 47 239 16.00 0.29 0.16 0.500676 -0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile early trophozoite, late trophozoite. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, gametocyte, early schizont, late ring, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 48 hs, late schizont, Sporozoite, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, Oocyst. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Ring. Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_128922)

Species Accession Gene Product
Arabidopsis thaliana AT5G66680   dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit
Brugia malayi Bm1_31915   Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDasubunit precursor
Candida albicans CaO19.9834   oligosaccharyl transferase, beta subunit
Candida albicans CaO19.2298   oligosaccharyl transferase, beta subunit
Caenorhabditis elegans CELE_T09A5.11   Protein OSTB-1
Cryptosporidium hominis Chro.20179   dolichyl-di-phosphooligosaccharide-protein glycotransferase (oligosaccharyltransferase)-related
Cryptosporidium parvum cgd2_1650   dolichyl-diphosphooligosaccharide-protein glycosyltransferase beta subunit (Wbp1p)
Dictyostelium discoideum DDB_G0291780   dolichyl-diphosphooligosaccharide-protein glycotransferase
Drosophila melanogaster Dmel_CG9022   Oligosaccharyltransferase 48kD subunit
Echinococcus granulosus EgrG_000525600   dolichyl diphosphooligosaccharide protein
Entamoeba histolytica EHI_069430   dolichyl-di-phosphooligosaccharide-protein glycotransferase, putative
Entamoeba histolytica EHI_028900   dolichyl-diphosphooligosaccharide-protein glycotransferase, putative
Echinococcus multilocularis EmuJ_000525600   dolichyl diphosphooligosaccharide protein
Homo sapiens ENSG00000244038   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Loa Loa (eye worm) LOAG_05287   dolichyl-di-phosphooligosaccharide-protein glycotransferase
Mus musculus ENSMUSG00000028757   dolichyl-di-phosphooligosaccharide-protein glycotransferase
Neospora caninum NCLIV_021120   dolichyl-di-phosphooligosaccharide-protein glycotransferase, putative
Oryza sativa 4342702   Os07g0209000
Plasmodium berghei PBANKA_0820500   dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative
Plasmodium falciparum PF3D7_0919600   dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative
Plasmodium knowlesi PKNH_0717600   dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative
Plasmodium vivax PVX_099395   dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative
Plasmodium yoelii PY05331   hypothetical protein
Saccharomyces cerevisiae YEL002C   Wbp1p
Schistosoma japonicum Sjp_0207160   ko:K00730 dolichyl-diphosphooligosaccharide--protein glycosyltransferase [EC2.4.1.119], putative
Schistosoma mansoni Smp_035470   dolichyl-diphosphooligosaccharide-protein glycosyltransferase
Toxoplasma gondii TGME49_203970   dolichyl-diphosphooligosaccharide--protein glycosyltransferase
Trichomonas vaginalis TVAG_059250   dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative

Essentiality

PF3D7_0919600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_T09A5.11 Caenorhabditis elegans embryonic lethal wormbase
CELE_T09A5.11 Caenorhabditis elegans larval arrest wormbase
CELE_T09A5.11 Caenorhabditis elegans larval lethal wormbase
CELE_T09A5.11 Caenorhabditis elegans slow growth wormbase
CELE_T09A5.11 Caenorhabditis elegans sterile wormbase
YEL002C Saccharomyces cerevisiae inviable yeastgenome
TGME49_203970 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0089 1 0.5
0.0108 1 0.5
0.0488 0.5 0.5
0.0176 1 0.5
0.0489 0.2944 1
0.0088 0.4411 0.5
0.0375 0.5 0.5
0.0549 1 0.5
0.0096 1 0.5
0.0088 0.4411 0.5
0.0489 0.2944 1
0.0094 0.729 1
0.0579 0.9567 1
0.0375 0.5 0.5
0.0089 0.5 0.5
0.0278 1 0.5
0.0489 0.2944 1
0.0089 1 0.5
0.0087 0.9495 1
0.0108 1 0.5
0.0094 0.5 0.5
0.049 1 1

Assayability

Assay information

  • Assay for Glycogen Synthase (2.4.1.11 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_0919600 (Plasmodium falciparum), dolichyl-diphosphooligosaccharide--protein glycosyltransferase, putative
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