Detailed view for EHI_178020

Basic information

TDR Targets ID: 804842
Entamoeba histolytica, leucine rich repeat / protein phosphatase 2C domain containing protein
Source Database / ID:  AmoebaDB  

pI: 7.9645 | Length (AA): 819 | MW (Da): 91831 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00481   Protein phosphatase 2C
PF13855   Leucine rich repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 611 3vq1 (A) 37 583 28.00 0.00000000019 1 0.787564 0.87
57 136 1xwd (C) 177 257 29.00 0 0.78 0.31518 0.2
576 819 4n0g (A) 104 390 28.00 0 1 0.685424 -1.31
682 815 2isn (A) 179 329 41.00 0.00000058 0.85 0.463914 0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129710)

Species Accession Gene Product
Candida albicans CaO19.5150   similar to S. cerevisiae CYR1 (YJL005W) adenylate cyclase
Candida albicans CaO19.5151   similar to S. cerevisiae CYR1 (YJL005W) adenylate cyclase
Candida albicans CaO19_5151   hypothetical protein
Candida albicans CaO19.12617   similar to S. cerevisiae CYR1 (YJL005W) adenylate cyclase
Caenorhabditis elegans CELE_F43C1.1   Protein F43C1.1
Drosophila melanogaster Dmel_CG10493   PH domain leucine-rich repeat protein phosphatase
Entamoeba histolytica EHI_178020   leucine rich repeat / protein phosphatase 2C domain containing protein
Homo sapiens ENSG00000081913   PH domain and leucine rich repeat protein phosphatase 1
Homo sapiens ENSG00000040199   PH domain and leucine rich repeat protein phosphatase 2
Loa Loa (eye worm) LOAG_04501   hypothetical protein
Mus musculus ENSMUSG00000044340   PH domain and leucine rich repeat protein phosphatase 1
Mus musculus ENSMUSG00000031732   PH domain and leucine rich repeat protein phosphatase 2
Saccharomyces cerevisiae YJL005W   adenylate cyclase
Trichomonas vaginalis TVAG_217790   protein phosphatase 2C, putative
Trichomonas vaginalis TVAG_410620   protein phosphatase type 2C, putative
Trichomonas vaginalis TVAG_339760   protein phosphatase 2C delta isoform, putative

Essentiality

EHI_178020 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
YJL005W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens PH domain and leucine rich repeat protein phosphatase 2 Compounds References
Homo sapiens PH domain and leucine rich repeat protein phosphatase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.014 0.394 0.5
0.0084 0.8373 1
0.0114 0.3811 1
0.014 0.2959 1
0.014 0.4497 0.5836
0.0084 0.8385 1
0.0121 0.4189 0.5
0.0088 0.6778 0.5
0.0113 0.6733 0.5
0.0117 0.7743 0.5
0.0115 0.4575 1
0.0113 0.6733 0.5
0.0124 0.6733 0.5
0.014 0.482 1
0.0117 0.3136 0.2779
0.014 0.4497 0.5836
0.0113 0.6683 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_178020 (Entamoeba histolytica), leucine rich repeat / protein phosphatase 2C domain containing protein
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