Detailed view for Rv2994

Basic information

TDR Targets ID: 8879
Mycobacterium tuberculosis, Probable conserved integral membrane protein
Source Database / ID:  Tuberculist 

pI: 11.7704 | Length (AA): 445 | MW (Da): 47046 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 8

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07690   Major Facilitator Superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0055085   transmembrane transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 405 1pw4 (A) 20 447 20.00 0 1 1.06437 0.58
10 393 3wdo (A) 13 392 18.00 0 1 1.06492 0.28
59 360 3wdo (A) 57 359 24.00 0.079 0.57 0.610952 1.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127815)

Species Accession Gene Product
Brugia malayi Bm1_07820   hypothetical protein
Caenorhabditis elegans CELE_F47B8.10   Protein F47B8.10
Chlamydia trachomatis CT_544   hexose phosphate transporter
Escherichia coli b2240   sn-glycerol-3-phosphate transporter
Escherichia coli b3667   membrane protein regulates uhpT expression
Giardia lamblia GL50803_9046   Sugar transport family protein
Homo sapiens ENSG00000137700   solute carrier family 37 (glucose-6-phosphate transporter), member 4
Mus musculus ENSMUSG00000032114   solute carrier family 37 (glucose-6-phosphate transporter), member 4
Mycobacterium tuberculosis Rv2994   Probable conserved integral membrane protein
Trichomonas vaginalis TVAG_042080   conserved hypothetical protein
Trichomonas vaginalis TVAG_388090   Hexuronate transporter, putative
Trichomonas vaginalis TVAG_044570   conserved hypothetical protein
Trichomonas vaginalis TVAG_097060   conserved hypothetical protein
Trichomonas vaginalis TVAG_329390   glucarate, hexuronate transporter, putative
Trichomonas vaginalis TVAG_147030   conserved hypothetical protein
Trichomonas vaginalis TVAG_253110   glucose-6-phosphate translocase, putative
Trichomonas vaginalis TVAG_240690   Regulatory protein uhpC, putative
Trichomonas vaginalis TVAG_458560   conserved hypothetical protein
Trichomonas vaginalis TVAG_241870   glucarate, hexuronate transporter, putative
Trichomonas vaginalis TVAG_250860   Regulatory protein uhpC, putative
Trichomonas vaginalis TVAG_283750   conserved hypothetical protein
Trichomonas vaginalis TVAG_308970   transport proetin, putative
Trichomonas vaginalis TVAG_229820   conserved hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0004   major facilitator superfamily permease

Essentiality

Rv2994 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3044 this record Mycobacterium tuberculosis non-essential nmpdr
b2240 Escherichia coli non-essential goodall
b3667 Escherichia coli non-essential goodall
CELE_F47B8.10 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens solute carrier family 37 (glucose-6-phosphate transporter), member 4 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Staphylococcus aureus Quinolone resistance protein norA 388 aa 22.0% 381 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0072 1 1
0.004 1 0.5
0.0106 1 0.5
0.0061 0.8617 1
0.0141 0.5229 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2994 (Mycobacterium tuberculosis), Probable conserved integral membrane protein
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