Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | Rho-associated, coiled-coil containing protein kinase 2 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | 55 kDa erythrocyte membrane protein | 0.0065 | 0.0226 | 0.0807 |
Trichomonas vaginalis | calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Mycobacterium tuberculosis | Probable guanylate kinase Gmk | 0.0054 | 0 | 0.5 |
Entamoeba histolytica | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Brugia malayi | Peripheral plasma membrane protein CASK | 0.0065 | 0.0226 | 0.0226 |
Echinococcus granulosus | membrane associated guanylate kinase ww and pdz | 0.0138 | 0.1697 | 0.6065 |
Brugia malayi | Guanylate kinase family protein | 0.0065 | 0.0226 | 0.0226 |
Echinococcus multilocularis | membrane associated guanylate kinase, WW and PDZ | 0.0138 | 0.1697 | 0.6065 |
Plasmodium vivax | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Echinococcus multilocularis | MAGUK p55 subfamily | 0.0065 | 0.0226 | 0.0807 |
Echinococcus multilocularis | membrane associated guanylate kinase ww and pdz | 0.0138 | 0.1697 | 0.6065 |
Trypanosoma cruzi | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0176 | 0.2472 | 0.8838 |
Echinococcus multilocularis | MAGUK p55 subfamily | 0.0065 | 0.0226 | 0.0807 |
Echinococcus granulosus | 3'partial|membrane associated guanylate kinase ww and pdz | 0.0138 | 0.1697 | 0.6065 |
Trichomonas vaginalis | sap-102, putative | 0.0054 | 0 | 0.5 |
Mycobacterium ulcerans | guanylate kinase | 0.0054 | 0 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0141 | 0.1763 | 0.1763 |
Loa Loa (eye worm) | peripheral plasma membrane protein CASK | 0.0065 | 0.0226 | 0.0226 |
Echinococcus multilocularis | disks large 3 | 0.0065 | 0.0226 | 0.0807 |
Mycobacterium leprae | Probable guanylate kinase Gmk (GMP kinase). | 0.0054 | 0 | 0.5 |
Echinococcus multilocularis | peripheral plasma membrane protein CASK | 0.0066 | 0.0233 | 0.0834 |
Schistosoma mansoni | maguk P55 subfamily member 26 | 0.0065 | 0.0226 | 0.0807 |
Echinococcus granulosus | MAGUK p55 subfamily | 0.0065 | 0.0226 | 0.0807 |
Loa Loa (eye worm) | hypothetical protein | 0.0065 | 0.0226 | 0.0226 |
Toxoplasma gondii | guanylate kinase family protein | 0.0054 | 0 | 0.5 |
Echinococcus granulosus | membrane associated guanylate kinase ww and pdz | 0.0192 | 0.2798 | 1 |
Schistosoma mansoni | maguk P55 subfamily member 35 | 0.0065 | 0.0226 | 0.0807 |
Schistosoma mansoni | cell polarity protein | 0.0065 | 0.0226 | 0.0807 |
Brugia malayi | Guanylate kinase family protein | 0.0065 | 0.0226 | 0.0226 |
Leishmania major | guanylate kinase-like protein | 0.0054 | 0 | 0.5 |
Schistosoma mansoni | MAGUK homolog | 0.0065 | 0.0226 | 0.0807 |
Schistosoma mansoni | MAGUK homolog | 0.0066 | 0.0233 | 0.0834 |
Trichomonas vaginalis | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Chlamydia trachomatis | guanylate kinase | 0.0054 | 0 | 0.5 |
Echinococcus granulosus | MAGUK p55 subfamily | 0.0065 | 0.0226 | 0.0807 |
Loa Loa (eye worm) | AGC/DMPK/ROCK protein kinase | 0.0546 | 1 | 1 |
Echinococcus multilocularis | 55 kDa erythrocyte membrane protein | 0.0065 | 0.0226 | 0.0807 |
Echinococcus multilocularis | MAGUK p55 subfamily | 0.0065 | 0.0226 | 0.0807 |
Brugia malayi | PDZ domain containing protein | 0.0141 | 0.1763 | 0.1763 |
Leishmania major | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Echinococcus granulosus | peripheral plasma membrane protein CASK | 0.0066 | 0.0233 | 0.0834 |
Echinococcus granulosus | MAGUK p55 subfamily | 0.0065 | 0.0226 | 0.0807 |
Trypanosoma brucei | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Wolbachia endosymbiont of Brugia malayi | guanylate kinase | 0.0054 | 0 | 0.5 |
Onchocerca volvulus | 0.0176 | 0.2472 | 1 | |
Echinococcus granulosus | disks large 3 | 0.0065 | 0.0226 | 0.0807 |
Trypanosoma cruzi | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Echinococcus granulosus | membrane associated guanylate kinase, WW and PDZ | 0.0138 | 0.1697 | 0.6065 |
Loa Loa (eye worm) | guanylate kinase | 0.0065 | 0.0226 | 0.0226 |
Schistosoma mansoni | ATP-dependent RNA Helicase | 0.0065 | 0.0226 | 0.0807 |
Trichomonas vaginalis | discs large, putative | 0.0054 | 0 | 0.5 |
Onchocerca volvulus | 0.0175 | 0.2465 | 0.9966 | |
Loa Loa (eye worm) | hypothetical protein | 0.0138 | 0.1697 | 0.1697 |
Trypanosoma brucei | guanylate kinase, putative | 0.0054 | 0 | 0.5 |
Schistosoma mansoni | membrane associated guanylate kinase inverted related | 0.0138 | 0.1697 | 0.6065 |
Schistosoma mansoni | membrane associated guanylate kinase inverted related | 0.0192 | 0.2798 | 1 |
Echinococcus multilocularis | rho associated protein kinase | 0.0176 | 0.2472 | 0.8838 |
Giardia lamblia | Guanylate kinase | 0.0054 | 0 | 0.5 |
Echinococcus multilocularis | 0.0192 | 0.2798 | 1 | |
Plasmodium falciparum | guanylate kinase | 0.0054 | 0 | 0.5 |
Echinococcus granulosus | rho-associated protein kinase 1 | 0.0176 | 0.2472 | 0.8838 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 0.35 nM | BindingDB_Patents: IMAP Assay. This assay is performed using FAM S6 substrate peptide (Catalogue #R7184) and IMAP FP Screening Express Kit detection reagents from Molecular Devices (Sunnyvale, Calif.) in IMAP kinase reaction buffer (Tris-HCl, pH 7.2, 10 mM MgCl2, 0.05% NaN3, 0.1% phosphate-free BSA) containing 1 mM DTT. Test compounds dissolved in neat DMSO at 0.3 mg/mL are serially diluted 1 to 3 for concentration response in 100% DMSO. The DMSO serial dilutions are further diluted 33.33-fold in kinase reaction buffer, and 10 µL of this buffer dilution is transferred to Corning black 96-well half area NBS plates for a final top concentration of 3 µg/mL in 1% DMSO. 10 µL aliquot of 3 nM ROCKII (1-543) diluted in kinase reaction buffer is added to each assay well for a final concentration of 1 nM kinase. 10 µL of a mixture of 600 nM FAM S6 peptide and 300 µM ATP diluted in kinase reaction buffer is added to each well for a final concentration of 200 nM peptide and 100 µM ATP. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.