Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | Rho-associated, coiled-coil containing protein kinase 2 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Giardia lamblia | Guanylate kinase | 0.0055 | 0 | 0.5 |
Schistosoma mansoni | MAGUK homolog | 0.0066 | 0.0236 | 0.0834 |
Plasmodium falciparum | guanylate kinase | 0.0055 | 0 | 0.5 |
Mycobacterium leprae | Probable guanylate kinase Gmk (GMP kinase). | 0.0055 | 0 | 0.5 |
Brugia malayi | Peripheral plasma membrane protein CASK | 0.0066 | 0.0228 | 0.0228 |
Schistosoma mansoni | membrane associated guanylate kinase inverted related | 0.0139 | 0.1715 | 0.6065 |
Echinococcus granulosus | membrane associated guanylate kinase, WW and PDZ | 0.0139 | 0.1715 | 0.6065 |
Echinococcus multilocularis | 55 kDa erythrocyte membrane protein | 0.0066 | 0.0228 | 0.0807 |
Toxoplasma gondii | guanylate kinase family protein | 0.0055 | 0 | 0.5 |
Brugia malayi | Guanylate kinase family protein | 0.0066 | 0.0228 | 0.0228 |
Echinococcus granulosus | membrane associated guanylate kinase ww and pdz | 0.0139 | 0.1715 | 0.6065 |
Wolbachia endosymbiont of Brugia malayi | guanylate kinase | 0.0055 | 0 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0066 | 0.0228 | 0.0228 |
Echinococcus multilocularis | rho associated protein kinase | 0.0176 | 0.2464 | 0.8714 |
Echinococcus multilocularis | membrane associated guanylate kinase, WW and PDZ | 0.0139 | 0.1715 | 0.6065 |
Trichomonas vaginalis | sap-102, putative | 0.0055 | 0 | 0.5 |
Echinococcus multilocularis | MAGUK p55 subfamily | 0.0066 | 0.0228 | 0.0807 |
Brugia malayi | PDZ domain containing protein | 0.0142 | 0.1782 | 0.1782 |
Trypanosoma cruzi | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Echinococcus granulosus | disks large 3 | 0.0066 | 0.0228 | 0.0807 |
Trypanosoma brucei | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Echinococcus granulosus | 55 kDa erythrocyte membrane protein | 0.0066 | 0.0228 | 0.0807 |
Echinococcus granulosus | MAGUK p55 subfamily | 0.0066 | 0.0228 | 0.0807 |
Echinococcus multilocularis | 0.0194 | 0.2828 | 1 | |
Schistosoma mansoni | ATP-dependent RNA Helicase | 0.0066 | 0.0228 | 0.0807 |
Schistosoma mansoni | maguk P55 subfamily member 35 | 0.0066 | 0.0228 | 0.0807 |
Entamoeba histolytica | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Echinococcus multilocularis | peripheral plasma membrane protein CASK | 0.0066 | 0.0236 | 0.0834 |
Echinococcus multilocularis | disks large 3 | 0.0066 | 0.0228 | 0.0807 |
Trypanosoma cruzi | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Echinococcus multilocularis | MAGUK p55 subfamily | 0.0066 | 0.0228 | 0.0807 |
Schistosoma mansoni | maguk P55 subfamily member 26 | 0.0066 | 0.0228 | 0.0807 |
Echinococcus multilocularis | MAGUK p55 subfamily | 0.0066 | 0.0228 | 0.0807 |
Chlamydia trachomatis | guanylate kinase | 0.0055 | 0 | 0.5 |
Trichomonas vaginalis | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Echinococcus granulosus | MAGUK p55 subfamily | 0.0066 | 0.0228 | 0.0807 |
Brugia malayi | Guanylate kinase family protein | 0.0066 | 0.0228 | 0.0228 |
Loa Loa (eye worm) | AGC/DMPK/ROCK protein kinase | 0.0546 | 1 | 1 |
Mycobacterium tuberculosis | Probable guanylate kinase Gmk | 0.0055 | 0 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0142 | 0.1782 | 0.1782 |
Loa Loa (eye worm) | peripheral plasma membrane protein CASK | 0.0066 | 0.0228 | 0.0228 |
Trypanosoma brucei | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Schistosoma mansoni | cell polarity protein | 0.0066 | 0.0228 | 0.0807 |
Echinococcus granulosus | peripheral plasma membrane protein CASK | 0.0066 | 0.0236 | 0.0834 |
Schistosoma mansoni | MAGUK homolog | 0.0066 | 0.0228 | 0.0807 |
Onchocerca volvulus | 0.0176 | 0.2464 | 1 | |
Leishmania major | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Echinococcus multilocularis | membrane associated guanylate kinase ww and pdz | 0.0139 | 0.1715 | 0.6065 |
Plasmodium vivax | guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Trichomonas vaginalis | discs large, putative | 0.0055 | 0 | 0.5 |
Echinococcus granulosus | MAGUK p55 subfamily | 0.0066 | 0.0228 | 0.0807 |
Loa Loa (eye worm) | guanylate kinase | 0.0066 | 0.0228 | 0.0228 |
Echinococcus granulosus | 3'partial|membrane associated guanylate kinase ww and pdz | 0.0139 | 0.1715 | 0.6065 |
Leishmania major | guanylate kinase-like protein | 0.0055 | 0 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0139 | 0.1715 | 0.1715 |
Onchocerca volvulus | 0.0175 | 0.2457 | 0.9966 | |
Trichomonas vaginalis | calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase, putative | 0.0055 | 0 | 0.5 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0176 | 0.2464 | 0.8714 |
Echinococcus granulosus | membrane associated guanylate kinase ww and pdz | 0.0194 | 0.2828 | 1 |
Schistosoma mansoni | membrane associated guanylate kinase inverted related | 0.0194 | 0.2828 | 1 |
Echinococcus granulosus | rho-associated protein kinase 1 | 0.0176 | 0.2464 | 0.8714 |
Mycobacterium ulcerans | guanylate kinase | 0.0055 | 0 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 22.5 nM | BindingDB_Patents: Luciferin-Luciferase Assay. The activity of ROCKII (1-543) kinase is measured utilizing Cambrex PKLight ATP Detection Reagent, a homogeneous assay technology using luciferin-luciferase to quantify residual ATP. The assay is performed in 384-well low-volume, white, non-binding surface microtiter plates (Corning). The assay buffer is 25 mM HEPES, pH 7.5, 10 mM MgCl2, 50 mM KCl, 0.2% BSA, 0.01% CHAPS, 100 µM Na3VO4 and 0.5 mM DTT. Test compounds, dissolved in neat DMSO at 500 µg/mL, are serially diluted for dose response for a final starting concentration of 3 µg/mL in 1% DMSO of assay buffer. ROCKII (1-543) (62,408 Da) is diluted in assay buffer to a final concentration of 7.5 nM in a total volume of 15 µL. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.