Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | purine nucleoside phosphorylase | Starlite/ChEMBL | No references |
Plasmodium falciparum | purine nucleoside phosphorylase | Starlite/ChEMBL | No references |
Species | Potential target | Known druggable target | Length | Alignment span | Identity |
---|---|---|---|---|---|
Trypanosoma brucei | methylthioadenosine phosphorylase, putative | purine nucleoside phosphorylase | 289 aa | 255 aa | 23.1 % |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Trichomonas vaginalis | purine nucleoside phosphorylase, putative | 0.0211 | 1 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Schistosoma mansoni | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 0.5 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Brugia malayi | purine nucleoside phosphorylase I, inosine and guanosine-specific family protein | 0.0186 | 0.7117 | 0.5 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Schistosoma mansoni | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 0.5 |
Mycobacterium leprae | Probable purine nucleoside phosphorylase DeoD (INOSINE PHOSPHORYLASE) (PNP) | 0.0186 | 0.7117 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0122 | 0 | 0.5 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Entamoeba histolytica | purine nucleoside phosphorylase, putative | 0.0211 | 1 | 0.5 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Giardia lamblia | Purine nucleoside phosphorylase lateral transfer candidate | 0.0186 | 0.7117 | 0.5 |
Trichomonas vaginalis | purine nucleoside phosphorylase, putative | 0.0211 | 1 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Treponema pallidum | uridine phosphorylase (udp) | 0.0211 | 1 | 0.5 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Plasmodium falciparum | purine nucleoside phosphorylase | 0.0211 | 1 | 0.5 |
Plasmodium vivax | purine nucleoside phosphorylase, putative | 0.0211 | 1 | 0.5 |
Treponema pallidum | purine nucleoside phosphorylase (deoD) | 0.0211 | 1 | 0.5 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 1 |
Mycobacterium tuberculosis | Probable purine nucleoside phosphorylase DeoD (inosine phosphorylase) (PNP) | 0.0186 | 0.7117 | 0.5 |
Mycobacterium ulcerans | purine nucleoside phosphorylase | 0.0186 | 0.7117 | 0.5 |
Toxoplasma gondii | Purine nucleoside phosphorylase | 0.0211 | 1 | 0.5 |
Onchocerca volvulus | Purine nucleoside phosphorylase homolog | 0.0186 | 0.7117 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
Kd (binding) | = 71 nM | Inhibition Assay | BINDINGDB. | No reference |
Kd (binding) | = 10000 nM | BindingDB_Patents: Inhibition Assay. The inhibitor dissociation constants reported in Table 1 below are for phosphorolysis of inosine by PNP and were based on reaction rates measurements with different inhibitor concentrations. Reactions were started by addition of 0.05 ug of human or Plasmodium falciparum purine nucleoside phosphaorylase (HsPNP and PfPNP, respectively; final concentration 1.4 nM) to 1 mM inosine in 50 mM KPO4, pH=7.5 buffer with xanthine oxidase added to final concentration 60 mU/mL at 25 C. In the coupled assay, hypoxanthine formed by phosphorolysis of inosine was oxidized to uric acid and followed spectrophotometrically at 293 nm (extinction coefficient for uric acid epsilon(293)=12.9 mM-1). The dissociation constant for slow-onset tight-binding inhibitors was determined from reaction rates after slow onset inhibition had occurred according to the equation u =(kcat x S)/(Km(1+l/Kd)+S), where u is the steady state reaction rate after the slow-onset inhibition period has reached equilibrium. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.