Detailed view for LmjF.06.1240

Basic information

TDR Targets ID: 27145
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1416 | Length (AA): 494 | MW (Da): 52681 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01494   FAD binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0071949   GO:FAD binding  

GO:0016709   oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen  
GO:0016491   oxidoreductase activity  
GO:0004497   monooxygenase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 474 1k0i (A) 1 386 14.00 0 0.98 0.78 0.15
20 470 2qa1 (A) 4 367 15.00 0 1 0.791955 0.71
374 404 2xdo (A) 308 338 48.00 0.95 0.07 0.355353 1.8

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128116)

Species Accession Gene Product
Arabidopsis thaliana AT3G24200   FAD/NAD(P)-binding oxidoreductase family protein
Candida albicans CaO19.3058   COQ6 monooxygenase, coenzyme Q biosynthesis
Candida albicans CaO19.10576   COQ6 monooxygenase, coenzyme Q biosynthesis
Caenorhabditis elegans CELE_K07B1.2   Protein COQ-6
Dictyostelium discoideum DDB_G0291440   hypothetical protein
Drosophila melanogaster Dmel_CG7277   CG7277 gene product from transcript CG7277-RA
Escherichia coli b0662   2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol oxygenase
Escherichia coli b2906   2-octaprenylphenol hydroxylase, FAD-dependent
Echinococcus granulosus EgrG_000422500   ubiquinone biosynthesis monooxygenase COQ6
Echinococcus multilocularis EmuJ_000422500   ubiquinone biosynthesis monooxygenase COQ6
Homo sapiens ENSG00000119723   coenzyme Q6 monooxygenase
Leishmania braziliensis LbrM.06.1230   hypothetical protein, conserved
Leishmania donovani LdBPK_061290.1   FAD binding domain containing protein, putative
Leishmania infantum LinJ.06.1290   hypothetical protein, conserved
Leishmania major LmjF.06.1240   hypothetical protein, conserved
Leishmania mexicana LmxM.06.1240   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_10177   hypothetical protein
Mus musculus ENSMUSG00000021235   coenzyme Q6 homolog (yeast)
Oryza sativa 4334721   Os03g0839100
Saccharomyces cerevisiae YGR255C   putative N,N-dimethylaniline monooxygenase COQ6
Schistosoma japonicum Sjp_0010040   ko:K06126 ubiquinone biosynthesis monooxygenase Coq6, putative
Schistosoma mansoni Smp_153460   monoxygenase
Schmidtea mediterranea mk4.003012.01   Probable ubiquinone biosynthesis monooxygenase coq-6
Schmidtea mediterranea mk4.003012.00   Probable ubiquinone biosynthesis monooxygenase coq-6
Trypanosoma brucei gambiense Tbg972.7.6780   Monooxygenase, putative
Trypanosoma brucei Tb927.7.5820   Monooxygenase, putative
Trypanosoma congolense TcIL3000_7_4820   Monooxygenase, putative
Trypanosoma cruzi TcCLB.508173.100   Monooxygenase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0064   2-polyprenyl-6-methoxyphenol 4-hydroxylase

Essentiality

LmjF.06.1240 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5820 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.5820 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.5820 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.5820 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0662 Escherichia coli essential goodall
b2906 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0353 0.5761 0.5
0.0171 0.5742 1
0.0219 1 0.5
0.0671 1 1
0.0095 0.5 0.5
0.0171 0.5742 1
0.0146 0.6701 0.5891
0.0224 0.3826 0.5
0.0206 1 1
0.0176 0.7425 1
0.0018 0.3365 1
0.0196 1 1
0.0086 1 0.5
0.0171 0.5742 1
0.0063 0.627 0.5
0.0219 1 0.5
0.0113 0.5252 0.5
0.0093 1 1
0.0116 1 0.5
0.0213 1 1
0.0066 1 1
0.0214 0.991 0.991
0.0271 1 1
0.0219 1 1
0.0111 1 0.5
0.0138 0.4448 0.5
0.015 1 1
0.0143 0.3221 0.3667
0.0139 0.3178 1
0.0079 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.06.1240 (Leishmania major), hypothetical protein, conserved
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