Detailed view for EHI_200020

Basic information

TDR Targets ID: 801287
Entamoeba histolytica, heat shock transcription factor, putative
Source Database / ID:  AmoebaDB  

pI: 7.3742 | Length (AA): 197 | MW (Da): 23352 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00447   HSF-type DNA-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0006355   regulation of transcription, DNA-dependent  
GO:0005634   nucleus  
GO:0043565   sequence-specific DNA binding  
GO:0003700   transcription factor activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 112 2ldu (A) 9 123 41.00 0 1 1.10355 -1.89
10 109 5hdk (A) 3 110 41.00 0 1 1.17801 -2.09
15 108 5d5w (B) 41 142 44.00 0 1 1.11356 -1.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129203)

Species Accession Gene Product
Arabidopsis thaliana AT4G36990   heat stress transcription factor B-1
Arabidopsis thaliana AT3G63350   heat stress transcription factor A-7b
Arabidopsis thaliana AT4G18880   heat stress transcription factor A-4a
Brugia malayi Bm1_17165   HSF-type DNA-binding domain containing protein
Candida albicans CaO19.4775   similar to S. cerevisiae Heat Shock Factor HSF1 (YGL073W) RNA Pol II transcriptional activator
Candida albicans CaO19.12238   similar to S. cerevisiae Heat Shock Factor HSF1 (YGL073W) RNA Pol II transcriptional activator
Caenorhabditis elegans CELE_Y53C10A.12   Protein HSF-1
Dictyostelium discoideum DDB_G0281165   heat shock factor -type DNA-binding domain-containing protein
Drosophila melanogaster Dmel_CG5748   Heat shock factor
Entamoeba histolytica EHI_200020   heat shock transcription factor, putative
Entamoeba histolytica EHI_049510   heat shock transcription factor, putative
Entamoeba histolytica EHI_087630   heat shock transcription factor, putative
Homo sapiens ENSG00000185122   heat shock transcription factor 1
Loa Loa (eye worm) LOAG_08901   hypothetical protein
Mus musculus ENSMUSG00000022556   heat shock factor 1
Onchocerca volvulus OVOC1424   Heat shock factor protein 2 homolog
Saccharomyces cerevisiae YGL073W   Hsf1p
Schmidtea mediterranea mk4.000226.05   Heat shock factor protein 2
Schmidtea mediterranea mk4.000226.06   Heat shock factor protein 2

Essentiality

EHI_200020 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y53C10A.12 Caenorhabditis elegans adult lethal wormbase
CELE_Y53C10A.12 Caenorhabditis elegans larval arrest wormbase
CELE_Y53C10A.12 Caenorhabditis elegans larval lethal wormbase
CELE_Y53C10A.12 Caenorhabditis elegans slow growth wormbase
CELE_Y53C10A.12 Caenorhabditis elegans sterile wormbase
YGL073W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens heat shock transcription factor 1 Compounds References
Mus musculus heat shock factor 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0097 0.2648 0.4889
0.0111 0.3929 0.5
0.011 0.392 0.5
0.0104 0.2887 0.2582
0.012 0.2593 0.2505
0.012 0.4152 1
0.012 0.2991 1
0.0098 0.3017 1
0.0324 0.3858 1
0.012 0.3044 1
0.0406 0.4174 1
0.0101 0.2689 1
0.0098 0.3456 1
0.0102 0.2705 0.5
0.0099 0.5426 1
0.012 0.3187 1
0.0564 1 0.5
0.0102 0.2543 0.5
0.012 0.2869 0.5
0.0098 0.5426 1
0.03 0.4008 1
0.012 0.3657 1
0.012 0.337 1
0.0097 0.6344 0.999
0.0112 0.3168 1
0.0101 0.2637 1
0.0568 0.5728 1
0.0096 0.4785 1
0.0111 0.4317 0.5
0.0101 0.5211 1
0.012 0.3187 1
0.0321 1 1
0.012 0.3015 0
0.0107 0.26 1
0.012 0.2623 1
0.052 0.2988 1
0.1265 0.3795 1
0.012 0.3061 1
0.0098 0.3456 1
0.012 0.2619 1
0.012 0.3145 1
0.01 0.2903 1
0.0103 0.2704 1
0.1135 0.3983 1
0.0106 0.3236 0.5
0.0441 0.5396 1
0.0096 0.267 1
0.0115 0.2958 1
0.0098 0.2619 0.5
0.032 0.414 1
0.012 0.3187 1
0.0101 0.3885 1
0.0114 1 0.5
0.012 0.3934 0.5486
0.0097 0.2664 1
0.0099 0.3633 0.919
0.0101 0.2637 1
0.012 1 1
0.012 0.4498 1
0.012 0.3657 1
0.0107 0.3061 1
0.0114 0.6046 0.5
0.012 0.2662 1
0.012 0.3182 1
0.012 1 1
0.012 0.599 1
0.0096 0.5426 1
0.0101 0.2689 1
0.1128 0.3814 1
0.012 0.3657 1
0.012 0.5476 0.5
0.103 0.385 1
0.0098 0.3012 1
0.012 0.6554 1
0.0066 0.3989 0.5127
0.103 0.385 1
0.1275 0.3821 1
0.0118 0.3114 1
0.0096 0.2543 0.5036
0.0099 0.2543 1
0.0524 0.6694 1
0.012 0.3657 1
0.0528 0.3558 0.6781
0.0101 0.5267 0.8355
0.0931 0.3837 1
0.012 0.3203 1
0.012 0.2557 1
0.0047741 0.5 0.5
0.0317 0.373 1
0.01 0.3927 1
0.0102 0.2556 1
0.012 0.3657 1
0.0432 0.5585 1
0.0299 0.3682 1
0.0071 0.2773 1
0.0098 0.2683 1
0.012 1 0.5
0.0101 0.3885 1
0.012 0.3096 0.5
0.0061 0.3957 0.5127
0.0102 0.4601 1
0.0317 0.373 1
0.0104 0.2639 1
0.0427 0.5976 1
0.0425 0.6072 1
0.012 0.2664 1
0.0109 0.2664 1
0.0114 0.2728 1
0.0093 0.3096 0.5
0.012 0.349 0.5
0.0108 0.4036 1
0.0317 0.373 1
0.1126 0.4025 1
0.0098 0.3456 1
0.0454 1 0.5
0.0102 0.5426 1
0.0116 0.2588 1
0.012 0.7811 1
0.0194 0.2771 1
0.0101 0.2689 1
0.012 0.3099 1
0.0294 0.3676 1
0.0116 0.5426 1
0.012 0.3189 1
0.0101 0.5588 1
0.012 0.2566 1
0.012 0.3657 1
0.0439 0.5492 1
0.011 0.2856 0.2248
0.012 0.3691 1
0.0115 0.3114 1
0.0099 0.345 1
0.1249 0.3825 1
0.0097 0.2509 1
0.0113 0.5426 1
0.011 1 0.5
0.012 0.2969 1
0.012 0.3675 0.5
0.012 0.3139 1
0.0194 0.2771 1
0.0114 0.5311 1
0.012 0.3657 1
0.012 0.3267 1
0.0114 0.3827 1
0.012 0.2557 1
0.0116 0.2625 0.2596
0.0329 0.8576 1
0.0531 0.2715 0.2865
0.0214 0.263 1
0.012 0.3934 1
0.012 0.2745 1
0.0329 0.8576 1
0.0111 0.3808 0.5
0.1158 0.4036 1
0.0204 0.3926 1
0.1265 0.3906 1
0.0116 0.3657 1
0.0116 0.3093 1
0.0436 1 1
0.012 0.3657 1
0.1128 0.3814 1
0.012 0.3254 1
0.0328 1 0.5
0.0105 0.3808 1
0.012 0.9071 1
0.012 0.3187 1
0.0105 0.2662 1
0.012 0.2543 1
0.0112 0.3554 1
0.0099 0.2788 1
0.012 0.3868 0.5
0.012 0.2971 0.2925
0.0102 0.3637 1
0.012 0.3203 1
0.1158 0.4036 1
0.012 0.5296 1
0.0097 1 0.5
0.012 0.3657 1
0.1139 0.3913 1
0.0102 0.277 1
0.011 0.3048 1
0.0109 0.4036 1
0.012 0.3657 1
0.0096 0.267 1
0.1044 0.3795 1
0.0097 0.5203 1
0.0102 0.3637 1
0.012 0.3099 1
0.0099 0.2761 1
0.012 0.3657 1
0.0103 0.2786 1
0.0098 0.3057 1
0.1135 0.3983 1
0.0542 0.5494 1
0.1123 0.3783 1
0.0112 0.2841 1
0.012 1 0.5
0.0441 1 1
0.0145 0.2648 0.9039
0.0074 0.2841 1
0.012 0.3657 1
0.0113 0.3914 1
0.0547 0.5 0.5
0.0097 0.313 1
0.0103 0.3934 1
0.0109 0.2588 1
0.012 0.6394 0.5
0.01 0.6394 0.5
0.012 0.8245 1
0.012 0.8147 1
0.012 0.3187 1
0.0082 0.282 1
0.012 1 0.5
0.0102 0.3011 1
0.0321 0.4132 1
0.0115 0.3568 0.5
0.0103 0.3809 0.3484
0.0099 0.2619 0.5
0.0113 0.2588 1
0.012 0.7791 1
0.0097 0.2971 1
0.012 0.3778 1
0.012 0.5396 1
0.0436 1 1
0.1396 0.3855 1
0.0203 0.3008 1
0.0551 0.7351 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_200020 (Entamoeba histolytica), heat shock transcription factor, putative
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