Detailed view for EHI_199120

Basic information

TDR Targets ID: 802826
Entamoeba histolytica, Ser/Thr protein phosphatase, putative
Source Database / ID:  AmoebaDB  

pI: 6.8402 | Length (AA): 525 | MW (Da): 61292 | Paralog Number: 5

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 434 4il1 (A) 271 797 38.00 0 1 1.23879 -0.33
52 466 4or9 (A) 19 491 35.00 0 1 1.16328 -0.2
57 427 5b8i (A) 31 388 40.00 0 1 1.22627 -0.7
85 404 3fga (C) 10 308 37.00 0 1 1.05712 -0.88
97 228 4iyp (C) 22 153 43.00 0.000000076 1 0.447129 0.5
414 516 4tql (A) 33 137 23.00 0.38 0.2 0.55389 -1.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127131)

Species Accession Gene Product
Brugia malayi Bm1_31320   Serine/threonine protein phosphatase 2B catalytic subunit 2
Candida albicans CaO19.6033   similar to calcineurin-like protein serine/threonine protein phosphatase
Candida albicans CaO19.13454   potential calcineurin-like protein serine/threonine protein phosphatase
Caenorhabditis elegans CELE_C02F4.2   Protein TAX-6, isoform C
Cryptosporidium hominis Chro.60481   protein phosphatase 2b
Cryptosporidium parvum cgd6_4200   protein phosphatase 2B catalytic subunit, calcineurin like phosphatse superfamily
Dictyostelium discoideum DDB_G0276883   CaM-dependent protein phosphatase, catalytic subunit
Drosophila melanogaster Dmel_CG9819   Calcineurin A at 14F
Drosophila melanogaster Dmel_CG9842   Protein phosphatase 2B at 14D
Drosophila melanogaster Dmel_CG1455   Calcineurin A1
Echinococcus granulosus EgrG_000601200   serine:threonine protein phosphatase 2B
Echinococcus granulosus EgrG_001157700   serine:threonine protein phosphatase 2B
Entamoeba histolytica EHI_199120   Ser/Thr protein phosphatase, putative
Entamoeba histolytica EHI_136190   Ser/Thr protein phosphatase, putative
Entamoeba histolytica EHI_051020   calcineurin catalytic subunit A, putative
Entamoeba histolytica EHI_167510   calcineurin catalytic subunit A, putative
Entamoeba histolytica EHI_146350   calcineurin catalytic subunit A, putative
Entamoeba histolytica EHI_151180   calcineurin catalytic subunit A, putative
Echinococcus multilocularis EmuJ_000601200   serine:threonine protein phosphatase 2B
Echinococcus multilocularis EmuJ_001157700   serine:threonine protein phosphatase 2B
Homo sapiens ENSG00000107758   protein phosphatase 3, catalytic subunit, beta isozyme
Homo sapiens ENSG00000138814   protein phosphatase 3, catalytic subunit, alpha isozyme
Homo sapiens ENSG00000120910   protein phosphatase 3, catalytic subunit, gamma isozyme
Leishmania braziliensis LbrM.35.2200   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Leishmania braziliensis LbrM.26.2470   serine/threonine protein phosphatase, putative
Leishmania donovani LdBPK_262560.1   serine/threonine protein phosphatase, putative
Leishmania donovani LdBPK_362090.1   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Leishmania infantum LinJ.26.2560   serine/threonine protein phosphatase, putative
Leishmania infantum LinJ.36.2090   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Leishmania major LmjF.26.2530   serine/threonine protein phosphatase, putative
Leishmania major LmjF.36.1980   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Leishmania mexicana LmxM.26.2530   serine/threonine protein phosphatase, putative
Leishmania mexicana LmxM.36.1980   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Loa Loa (eye worm) LOAG_06644   hypothetical protein
Loa Loa (eye worm) LOAG_13776   hypothetical protein
Mus musculus ENSMUSG00000021816   protein phosphatase 3, catalytic subunit, beta isoform
Mus musculus ENSMUSG00000022092   protein phosphatase 3, catalytic subunit, gamma isoform
Mus musculus ENSMUSG00000028161   protein phosphatase 3, catalytic subunit, alpha isoform
Neospora caninum NCLIV_055230   serine/threonine protein phosphatase, putative
Plasmodium berghei PBANKA_1227400   serine/threonine protein phosphatase 2B catalytic subunit A
Plasmodium falciparum PF3D7_0802800   serine/threonine protein phosphatase 2B catalytic subunit A
Plasmodium knowlesi PKNH_0116100   serine/threonine protein phosphatase 2B catalytic subunit A, putative
Plasmodium vivax PVX_093605   serine/threonine protein phosphatase 2B catalytic subunit A, putative
Plasmodium yoelii PY02315   protein phosphatase 2B-related
Saccharomyces cerevisiae YML057W   calcineurin catalytic subunit A
Saccharomyces cerevisiae YLR433C   calcineurin catalytic subunit A
Schistosoma japonicum Sjp_0053500   Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, putative
Schistosoma japonicum Sjp_0063220   Serine/threonine-protein phosphatase 2B catalytic subunit 3, putative
Schistosoma japonicum Sjp_0114900   expressed protein
Schistosoma japonicum Sjp_0098580   Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, putative
Schistosoma japonicum Sjp_0053510   Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, putative
Schistosoma japonicum Sjp_0300900   ko:K04348 protein phosphatase 3, catalytic subunit, putative
Schistosoma mansoni Smp_141950   protein phosphatase-2b
Schistosoma mansoni Smp_126260   protein phosphatase-2b
Schmidtea mediterranea mk4.017776.00  
Schmidtea mediterranea mk4.001455.00  
Schmidtea mediterranea mk4.012070.00  
Schmidtea mediterranea mk4.001142.14  
Schmidtea mediterranea mk4.003344.00  
Schmidtea mediterranea mk4.001142.15  
Schmidtea mediterranea mk4.005570.01  
Schmidtea mediterranea mk4.019522.00  
Schmidtea mediterranea mk4.001142.16  
Trypanosoma brucei gambiense Tbg972.10.7910   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Trypanosoma brucei Tb927.10.6460   protein phosphatase 2B, putative
Trypanosoma congolense TcIL3000_10_5520   protein phosphatase 2B, putative
Trypanosoma cruzi TcCLB.510187.500   serine/threonine protein phosphatase 2B catalytic subunit A2, putative
Trypanosoma cruzi TcCLB.508413.40   serine/threonine protein phosphatase, putative
Trypanosoma cruzi TcCLB.510755.138   serine/threonine protein phosphatase, putative
Toxoplasma gondii TGME49_311310   protein phosphatase 2B catalytic subunit, calcineurin family phosphatse superfamily protein

Essentiality

EHI_199120 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.6460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.6460 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.6460 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.6460 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C02F4.2 Caenorhabditis elegans slow growth wormbase
PBANKA_1227400 Plasmodium berghei Essential plasmo
TGME49_311310 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein phosphatase 3, catalytic subunit, alpha isozyme Compounds References
Candida dubliniensis (strain CD36 / CBS 7987 / NCPF 3949 / NRRLY-17841) (Yeast) Serine/threonine-protein phosphatase Compounds References
Schistosoma mansoni protein phosphatase-2b Compounds References
Homo sapiens protein phosphatase 3, catalytic subunit, beta isozyme Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform 309 aa 37.2% 277 aa Compounds References
Oryctolagus cuniculus Serine/threonine protein phosphatase PP1-alpha catalytic subunit 330 aa 38.2% 283 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0042 0.5 0.5
0.0583 0.2972 1
0.0041 1 1
0.0041 0.2965 0.2952
0.0309 0.3429 1
0.0756 0.3955 1
0.0046 0.326 1
0.0312 0.2512 0.2502
0.054 0.2709 1
0.0577 0.2963 1
0.0583 0.2968 1
0.0171 0.5083 0.5025
0.0577 0.2963 1
0.049 0.2898 1
0.0728 0.3956 1
0.0198 0.6459 0.5
0.0187 0.4379 1
0.0591 0.2969 1
0.0051 0.6475 0.571
0.0692 0.3956 1
0.0584 0.297 1
0.0051 0.7066 0
0.0756 0.3956 1
0.0046 0.326 1
0.0738 0.3953 1
0.0046 0.326 1
0.0042 1 0.5
0.0577 0.2972 1
0.0042 1 1
0.054 0.2709 1
0.0092 0.402 0.4022
0.0584 0.2973 1
0.054 0.2974 1
0.0046 0.326 1
0.0577 0.2963 1
0.0577 0.2972 1
0.0051 0.2978 0.5
0.0584 0.2973 1
0.0148 1 0.5
0.054 0.2974 1
0.0046 1 1
0.0192 0.9291 1
0.0584 0.2973 1
0.0397099 0.653295 0.653295
0.013 0.3141 0.5
0.0584 0.2972 1
0.0746 0.3953 1
0.0051 1 0.5
0.0692 0.3956 1
0.0728 0.3956 1
0.0043 0.5 0.5
0.049 0.2898 1
0.0127 0.4475 0
0.0089 0.3172 1
0.057 0.2974 1
0.0193 0.7322 0
0.0583 0.2968 1
0.0089 0.7409 0.7409
0.0563 0.2972 1
0.0536 0.2974 1
0.0316 0.3557 1
0.0584 0.2973 1
0.0584 0.2973 1
0.0577 0.2963 1
0.0584 0.2973 1
0.0085 0.7315 0.7307
0.0577 0.2972 1
0.0051 0.7066 0
0.0583 0.2968 1
0.0756 0.3955 1
0.0738 0.3953 1
0.0591 0.2969 1
0.0756 0.3955 1
0.0293 1 1
0.0756 0.3955 1
0.0047 1 1
0.0534 0.32 1
0.0045 0.5 0.5
0.0756 0.3956 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_199120 (Entamoeba histolytica), Ser/Thr protein phosphatase, putative
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