Detailed view for MUL_3266

Basic information

TDR Targets ID: 951600
Mycobacterium ulcerans, methyltransferase
Source Database / ID:  KEGG  

pI: 10.3615 | Length (AA): 274 | MW (Da): 29243 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08241   Methyltransferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
23 269 4ine (A) 194 434 16.00 0 1 0.97186 0.41
24 269 3bus (A) 31 273 23.00 0 0.96 1.10301 0.35
41 155 1ve3 (A) 25 141 24.00 0 0.68 0.743108 -0.54
57 166 4hg2 (A) 39 141 35.00 0.38 0.7 0.70336 0.3
58 130 1g6q (1) 62 138 16.00 0.17 0.11 0.289823 0.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_132295)

Species Accession Gene Product
Arabidopsis thaliana AT3G18000   phosphoethanolamine N-methyltransferase 1
Arabidopsis thaliana AT1G73600   putative phosphoethanolamine N-methyltransferase 3
Arabidopsis thaliana AT1G48600   phosphoethanolamine N-methyltransferase 2
Caenorhabditis elegans CELE_F54D11.1   Protein PMT-2
Dictyostelium discoideum DDB_G0275359   hypothetical protein
Mycobacterium tuberculosis Rv0469   Possible mycolic acid synthase UmaA
Mycobacterium tuberculosis Rv2622   Possible methyltransferase (methylase)
Mycobacterium ulcerans MUL_3266   methyltransferase
Mycobacterium ulcerans MUL_4538   mycolic acid synthase UmaA
Oryza sativa 4339516   Os05g0548900
Plasmodium falciparum PF3D7_1343000   phosphoethanolamine N-methyltransferase
Plasmodium knowlesi PKNH_1258400   phosphoethanolamine N-methyltransferase
Plasmodium vivax PVX_083045   phosphoethanolamine N-methyltransferase

Essentiality

MUL_3266 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu2668 Mycobacterium tuberculosis non-essential nmpdr
mtu473 Mycobacterium tuberculosis non-essential nmpdr
CELE_F54D11.1 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum phosphoethanolamine N-methyltransferase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0159 0.2533 1
0.0163 0.877 1
0.0134 0.3769 1
0.0152 0.8767 1
0.015 0.9384 1
0.0144 0.9384 1
0.0139 0.5369 1
0.0137 0.9384 1
0.0139 0.9381 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier MUL_3266 (Mycobacterium ulcerans), methyltransferase
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