Detailed view for TcCLB.509011.100

Basic information

TDR Targets ID: 33758
Trypanosoma cruzi, myosin heavy chain kinase A, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0607 | Length (AA): 329 | MW (Da): 37422 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02816   Alpha-kinase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 276 1ia9 (A) 1585 1824 24.00 0 1 0.66 0.59
160 274 1ia9 (A) 1715 1822 36.00 0.0000000000047 0.99 0.63 0.12
47 285 4kuj (A) 59 280 27.00 0 1 0.934644 0.13
52 271 4kuj (A) 64 264 32.00 0 1 0.818693 0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_130980)

Species Accession Gene Product
Brugia malayi Bm1_35010   MHCK/EF2 kinase domain family protein
Caenorhabditis elegans CELE_F42A10.4   Protein EFK-1, isoform B
Dictyostelium discoideum DDB_G0292150   Alpha kinase family protein
Echinococcus granulosus EgrG_000237000   eukaryotic elongation factor 2 kinase
Echinococcus multilocularis EmuJ_000237000   eukaryotic elongation factor 2 kinase
Homo sapiens 101930123   eukaryotic elongation factor 2 kinase-like
Homo sapiens ENSG00000103319   eukaryotic elongation factor 2 kinase
Leishmania braziliensis LbrM.35.4800   myosin heavy chain kinase a-like protein
Leishmania donovani LdBPK_364780.1   myosin heavy chain kinase a-like protein
Leishmania infantum LinJ.36.4780   myosin heavy chain kinase a-like protein
Leishmania major LmjF.36.4700   myosin heavy chain kinase a-like protein
Leishmania mexicana LmxM.36.4551   myosin heavy chain kinase a-like protein
Loa Loa (eye worm) LOAG_04841   hypothetical protein
Mus musculus ENSMUSG00000035064   eukaryotic elongation factor-2 kinase
Schistosoma japonicum Sjp_0128290   Elongation factor 2 kinase, putative
Schistosoma japonicum Sjp_0087030   ko:K08292 elongation factor 2 kinase [EC2.7.11.20], putative
Schistosoma japonicum Sjp_0309020   expressed protein
Schistosoma mansoni Smp_072980   eukaryotic elongation factor 2 kinase (eef-2 kinase)
Schmidtea mediterranea mk4.006866.00   Eukaryotic elongation factor 2 kinase
Schmidtea mediterranea mk4.001647.04  
Schmidtea mediterranea mk4.011939.00   Mucolipin, putative
Schmidtea mediterranea mk4.000429.02  
Schmidtea mediterranea mk4.001647.02   Mucolipin, putative
Schmidtea mediterranea mk4.000429.04  
Schmidtea mediterranea mk4.006866.01   Eukaryotic elongation factor 2 kinase
Schmidtea mediterranea mk4.001647.05  
Trypanosoma brucei gambiense Tbg972.8.7690   myosin heavy chain kinase A, putative
Trypanosoma brucei gambiense Tbg972.4.5150   myosin heavy chain kinase A, putative
Trypanosoma brucei Tb927.8.7450   myosin heavy chain kinase A, putative
Trypanosoma brucei Tb927.4.4970   myosin heavy chain kinase A, putative
Trypanosoma congolense TcIL3000_8_7840   myosin heavy chain kinase A, putative
Trypanosoma congolense TcIL3000_8_7690   myosin heavy chain kinase A, putative
Trypanosoma congolense TcIL3000_4_4190   myosin heavy chain kinase A, putative
Trypanosoma cruzi TcCLB.510681.20   myosin heavy chain kinase A, putative
Trypanosoma cruzi TcCLB.509011.100   myosin heavy chain kinase A, putative

Essentiality

TcCLB.509011.100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.7450 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.7450 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.7450 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.7450 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.4.4970 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.4970 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.4970 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.4970 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens eukaryotic elongation factor 2 kinase-like Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0073 0.5 0.5
0.0109 0.5503 0.9894
0.0139 0.7305 1
0.0112 0.5407 0.9713
0.0039 0.819 0.5
0.0028 0.819 0.5
0.0112 0.6467 0.5
0.0112 0.5407 0.9713
0.0134 0.7545 1
0.0071 0.3321 0.543
0.0093 0.5454 0.9841
0.0213843 0.280656 0.296443
0.0129 0.7305 1
0.0062 0.3542 0.5588
0.0213843 0.280656 0.296443
0.00423136 1 0.5
0.0213843 0.280656 0.296443
0.0111 0.5406 0.9676
0.0213843 0.280656 0.296443
0.0147 0.7466 1
0.00440155 1 0.5
0.0067 0.3631 1
0.0031 0.819 0.5
0.0112 0.5407 0.9713
0.0128 0.7298 1

Assayability

Assay information

  • Assay for Gluconate Kinase (2.7.1.12 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TcCLB.509011.100 (Trypanosoma cruzi), myosin heavy chain kinase A, putative
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